Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid
The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs)...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818501/ https://www.ncbi.nlm.nih.gov/pubmed/36611843 http://dx.doi.org/10.3390/cells12010049 |
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author | Ruiz-García, Ana Belén Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry |
author_facet | Ruiz-García, Ana Belén Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry |
author_sort | Ruiz-García, Ana Belén |
collection | PubMed |
description | The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs) from Israel provided evidence for the presence of similar ADFVd isolates in pomegranate trees in this country. In addition, reads or contigs of plum viroid I (PVd-I) isolates were also identified in two of the analyzed SRA datasets from Israel, suggesting the presence of this second viroid in pomegranate. Full length ADFVd genomic sequences have been recovered, increasing knowledge on the diversity of this viroid and on the pomegranate virome in which only four viruses and one viroid had previously been reported. |
format | Online Article Text |
id | pubmed-9818501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98185012023-01-07 Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid Ruiz-García, Ana Belén Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry Cells Communication The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs) from Israel provided evidence for the presence of similar ADFVd isolates in pomegranate trees in this country. In addition, reads or contigs of plum viroid I (PVd-I) isolates were also identified in two of the analyzed SRA datasets from Israel, suggesting the presence of this second viroid in pomegranate. Full length ADFVd genomic sequences have been recovered, increasing knowledge on the diversity of this viroid and on the pomegranate virome in which only four viruses and one viroid had previously been reported. MDPI 2022-12-22 /pmc/articles/PMC9818501/ /pubmed/36611843 http://dx.doi.org/10.3390/cells12010049 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Ruiz-García, Ana Belén Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
title | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
title_full | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
title_fullStr | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
title_full_unstemmed | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
title_short | Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid |
title_sort | natural infection of pomegranate (punica granatum) by apple dimple fruit viroid |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818501/ https://www.ncbi.nlm.nih.gov/pubmed/36611843 http://dx.doi.org/10.3390/cells12010049 |
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