Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid

The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs)...

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Autores principales: Ruiz-García, Ana Belén, Olmos, Antonio, Marais, Armelle, Faure, Chantal, Candresse, Thierry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818501/
https://www.ncbi.nlm.nih.gov/pubmed/36611843
http://dx.doi.org/10.3390/cells12010049
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author Ruiz-García, Ana Belén
Olmos, Antonio
Marais, Armelle
Faure, Chantal
Candresse, Thierry
author_facet Ruiz-García, Ana Belén
Olmos, Antonio
Marais, Armelle
Faure, Chantal
Candresse, Thierry
author_sort Ruiz-García, Ana Belén
collection PubMed
description The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs) from Israel provided evidence for the presence of similar ADFVd isolates in pomegranate trees in this country. In addition, reads or contigs of plum viroid I (PVd-I) isolates were also identified in two of the analyzed SRA datasets from Israel, suggesting the presence of this second viroid in pomegranate. Full length ADFVd genomic sequences have been recovered, increasing knowledge on the diversity of this viroid and on the pomegranate virome in which only four viruses and one viroid had previously been reported.
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spelling pubmed-98185012023-01-07 Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid Ruiz-García, Ana Belén Olmos, Antonio Marais, Armelle Faure, Chantal Candresse, Thierry Cells Communication The analysis by high throughput sequencing (HTS) and RT-PCR of Spanish pomegranate fruits showing yellow rings revealed the presence of viroid isolates closely related to fig isolates of apple dimple fruit viroid (ADFVd). The analysis of pomegranate public RNASeq data (Sequence Reads Archives, SRAs) from Israel provided evidence for the presence of similar ADFVd isolates in pomegranate trees in this country. In addition, reads or contigs of plum viroid I (PVd-I) isolates were also identified in two of the analyzed SRA datasets from Israel, suggesting the presence of this second viroid in pomegranate. Full length ADFVd genomic sequences have been recovered, increasing knowledge on the diversity of this viroid and on the pomegranate virome in which only four viruses and one viroid had previously been reported. MDPI 2022-12-22 /pmc/articles/PMC9818501/ /pubmed/36611843 http://dx.doi.org/10.3390/cells12010049 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ruiz-García, Ana Belén
Olmos, Antonio
Marais, Armelle
Faure, Chantal
Candresse, Thierry
Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid
title Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid
title_full Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid
title_fullStr Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid
title_full_unstemmed Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid
title_short Natural Infection of Pomegranate (Punica Granatum) by Apple Dimple Fruit Viroid
title_sort natural infection of pomegranate (punica granatum) by apple dimple fruit viroid
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818501/
https://www.ncbi.nlm.nih.gov/pubmed/36611843
http://dx.doi.org/10.3390/cells12010049
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