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Rhinovirus Suppresses TGF-β-GARP Presentation by Peripheral NK Cells

Asthma is a chronic airway disease whose exacerbations are often triggered by rhinovirus infection. TGF-β1 induces rhinovirus replication in infected cells. Moreover, TGF-β1 is a pleiotropic mediator that is produced by many immune cells in the latent, inactive form bound to the latency-associated p...

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Autores principales: Krammer, Susanne, Yang, Zuqin, Mitländer, Hannah, Grund, Janina C., Trump, Sonja, Mittler, Susanne, Zirlik, Sabine, Finotto, Susetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818541/
https://www.ncbi.nlm.nih.gov/pubmed/36611921
http://dx.doi.org/10.3390/cells12010129
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author Krammer, Susanne
Yang, Zuqin
Mitländer, Hannah
Grund, Janina C.
Trump, Sonja
Mittler, Susanne
Zirlik, Sabine
Finotto, Susetta
author_facet Krammer, Susanne
Yang, Zuqin
Mitländer, Hannah
Grund, Janina C.
Trump, Sonja
Mittler, Susanne
Zirlik, Sabine
Finotto, Susetta
author_sort Krammer, Susanne
collection PubMed
description Asthma is a chronic airway disease whose exacerbations are often triggered by rhinovirus infection. TGF-β1 induces rhinovirus replication in infected cells. Moreover, TGF-β1 is a pleiotropic mediator that is produced by many immune cells in the latent, inactive form bound to the latency-associated peptide (LAP) and to the transmembrane protein glycoprotein A repetitions predominant (GARP). In this study we wanted to investigate the effect of rhinovirus infection on the TGF-β secretion and the downstream signaling via TGF-βRI/RII in peripheral blood mononuclear cells from control and asthmatic patients after rhinovirus infection ex vivo. Here, we found a significant upregulation of TGF-βRII in untouched PBMCs of asthmatics as well as a suppression of TGF-β release in the rhinovirus-infected PBMC condition. Moreover, consistent with an effect of TGF-β on Tregs, PBMCs infected with RV induced Tregs, and TGF-βRII directly correlated with RV1b mRNA. Finally, we found via flow cytometry that NK cells expressed less GARP surface-bound TGF-β, while cytokine-producing NK(bright) cells were induced. In summary, we show that rhinovirus infection inhibits TGF-β release in PBMCs, which results in the activation of both Treg and NK cells.
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spelling pubmed-98185412023-01-07 Rhinovirus Suppresses TGF-β-GARP Presentation by Peripheral NK Cells Krammer, Susanne Yang, Zuqin Mitländer, Hannah Grund, Janina C. Trump, Sonja Mittler, Susanne Zirlik, Sabine Finotto, Susetta Cells Article Asthma is a chronic airway disease whose exacerbations are often triggered by rhinovirus infection. TGF-β1 induces rhinovirus replication in infected cells. Moreover, TGF-β1 is a pleiotropic mediator that is produced by many immune cells in the latent, inactive form bound to the latency-associated peptide (LAP) and to the transmembrane protein glycoprotein A repetitions predominant (GARP). In this study we wanted to investigate the effect of rhinovirus infection on the TGF-β secretion and the downstream signaling via TGF-βRI/RII in peripheral blood mononuclear cells from control and asthmatic patients after rhinovirus infection ex vivo. Here, we found a significant upregulation of TGF-βRII in untouched PBMCs of asthmatics as well as a suppression of TGF-β release in the rhinovirus-infected PBMC condition. Moreover, consistent with an effect of TGF-β on Tregs, PBMCs infected with RV induced Tregs, and TGF-βRII directly correlated with RV1b mRNA. Finally, we found via flow cytometry that NK cells expressed less GARP surface-bound TGF-β, while cytokine-producing NK(bright) cells were induced. In summary, we show that rhinovirus infection inhibits TGF-β release in PBMCs, which results in the activation of both Treg and NK cells. MDPI 2022-12-28 /pmc/articles/PMC9818541/ /pubmed/36611921 http://dx.doi.org/10.3390/cells12010129 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krammer, Susanne
Yang, Zuqin
Mitländer, Hannah
Grund, Janina C.
Trump, Sonja
Mittler, Susanne
Zirlik, Sabine
Finotto, Susetta
Rhinovirus Suppresses TGF-β-GARP Presentation by Peripheral NK Cells
title Rhinovirus Suppresses TGF-β-GARP Presentation by Peripheral NK Cells
title_full Rhinovirus Suppresses TGF-β-GARP Presentation by Peripheral NK Cells
title_fullStr Rhinovirus Suppresses TGF-β-GARP Presentation by Peripheral NK Cells
title_full_unstemmed Rhinovirus Suppresses TGF-β-GARP Presentation by Peripheral NK Cells
title_short Rhinovirus Suppresses TGF-β-GARP Presentation by Peripheral NK Cells
title_sort rhinovirus suppresses tgf-β-garp presentation by peripheral nk cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818541/
https://www.ncbi.nlm.nih.gov/pubmed/36611921
http://dx.doi.org/10.3390/cells12010129
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