Cargando…

Cell Dome as an Evaluation Platform for Organized HepG2 Cells

Human-hepatoblastoma-derived cell line, HepG2, has been widely used in liver and liver cancer studies. HepG2 spheroids produced in a three-dimensional (3D) culture system provide a better biological model than cells cultured in a two-dimensional (2D) culture system. Since cells at the center of sphe...

Descripción completa

Detalles Bibliográficos
Autores principales: Kazama, Ryotaro, Fujita, Satoshi, Sakai, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818560/
https://www.ncbi.nlm.nih.gov/pubmed/36611862
http://dx.doi.org/10.3390/cells12010069
_version_ 1784865016058478592
author Kazama, Ryotaro
Fujita, Satoshi
Sakai, Shinji
author_facet Kazama, Ryotaro
Fujita, Satoshi
Sakai, Shinji
author_sort Kazama, Ryotaro
collection PubMed
description Human-hepatoblastoma-derived cell line, HepG2, has been widely used in liver and liver cancer studies. HepG2 spheroids produced in a three-dimensional (3D) culture system provide a better biological model than cells cultured in a two-dimensional (2D) culture system. Since cells at the center of spheroids exhibit specific behaviors attributed to hypoxic conditions, a 3D cell culture system that allows the observation of such cells using conventional optical or fluorescence microscopes would be useful. In this study, HepG2 cells were cultured in “Cell Dome”, a micro-dome in which cells are enclosed in a cavity consisting of a hemispherical hydrogel shell. HepG2 cells formed hemispherical cell aggregates which filled the cavity of Cell Domes on 18 days of culture and the cells could continue to be cultured for 29 days. The cells at the center of hemispherical cell aggregates were observed using a fluorescence microscope. The cells grew in Cell Domes for 18 days exhibited higher Pi-class Glutathione S-Transferase enzymatic activity, hypoxia inducible factor-1α gene expression, and higher tolerance to mitomycin C than those cultured in 2D on tissue culture dishes (* p < 0.05). These results indicate that the center of the glass adhesive surface of hemispherical cell aggregates which is expected to have the similar environment as the center of the spheroids can be directly observed through glass plates. In conclusion, Cell Dome would be useful as an evaluation platform for organized HepG2 cells.
format Online
Article
Text
id pubmed-9818560
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98185602023-01-07 Cell Dome as an Evaluation Platform for Organized HepG2 Cells Kazama, Ryotaro Fujita, Satoshi Sakai, Shinji Cells Article Human-hepatoblastoma-derived cell line, HepG2, has been widely used in liver and liver cancer studies. HepG2 spheroids produced in a three-dimensional (3D) culture system provide a better biological model than cells cultured in a two-dimensional (2D) culture system. Since cells at the center of spheroids exhibit specific behaviors attributed to hypoxic conditions, a 3D cell culture system that allows the observation of such cells using conventional optical or fluorescence microscopes would be useful. In this study, HepG2 cells were cultured in “Cell Dome”, a micro-dome in which cells are enclosed in a cavity consisting of a hemispherical hydrogel shell. HepG2 cells formed hemispherical cell aggregates which filled the cavity of Cell Domes on 18 days of culture and the cells could continue to be cultured for 29 days. The cells at the center of hemispherical cell aggregates were observed using a fluorescence microscope. The cells grew in Cell Domes for 18 days exhibited higher Pi-class Glutathione S-Transferase enzymatic activity, hypoxia inducible factor-1α gene expression, and higher tolerance to mitomycin C than those cultured in 2D on tissue culture dishes (* p < 0.05). These results indicate that the center of the glass adhesive surface of hemispherical cell aggregates which is expected to have the similar environment as the center of the spheroids can be directly observed through glass plates. In conclusion, Cell Dome would be useful as an evaluation platform for organized HepG2 cells. MDPI 2022-12-23 /pmc/articles/PMC9818560/ /pubmed/36611862 http://dx.doi.org/10.3390/cells12010069 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kazama, Ryotaro
Fujita, Satoshi
Sakai, Shinji
Cell Dome as an Evaluation Platform for Organized HepG2 Cells
title Cell Dome as an Evaluation Platform for Organized HepG2 Cells
title_full Cell Dome as an Evaluation Platform for Organized HepG2 Cells
title_fullStr Cell Dome as an Evaluation Platform for Organized HepG2 Cells
title_full_unstemmed Cell Dome as an Evaluation Platform for Organized HepG2 Cells
title_short Cell Dome as an Evaluation Platform for Organized HepG2 Cells
title_sort cell dome as an evaluation platform for organized hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818560/
https://www.ncbi.nlm.nih.gov/pubmed/36611862
http://dx.doi.org/10.3390/cells12010069
work_keys_str_mv AT kazamaryotaro celldomeasanevaluationplatformfororganizedhepg2cells
AT fujitasatoshi celldomeasanevaluationplatformfororganizedhepg2cells
AT sakaishinji celldomeasanevaluationplatformfororganizedhepg2cells