Cargando…

Prognostic Utility of CD47 in Cancer of the Uterine Cervix and the Sensitivity of Immunohistochemical Scores

Introduction: Cancer of the uterine cervix (CUC) is still one of the most frequent oncological diagnoses in women. The specific interactions between the tumor cells of CUC and the cells and tissues in the tumor microenvironment can affect cancer cells’ invasive and metastatic potential and can modul...

Descripción completa

Detalles Bibliográficos
Autores principales: Yordanov, Angel, Shivarov, Velizar, Kostov, Stoyan, Ivanova, Yonka, Dimitrova, Polina, Popovska, Savelina, Tsoneva, Eva, Vasileva-Slaveva, Mariela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818840/
https://www.ncbi.nlm.nih.gov/pubmed/36611344
http://dx.doi.org/10.3390/diagnostics13010052
_version_ 1784865084536782848
author Yordanov, Angel
Shivarov, Velizar
Kostov, Stoyan
Ivanova, Yonka
Dimitrova, Polina
Popovska, Savelina
Tsoneva, Eva
Vasileva-Slaveva, Mariela
author_facet Yordanov, Angel
Shivarov, Velizar
Kostov, Stoyan
Ivanova, Yonka
Dimitrova, Polina
Popovska, Savelina
Tsoneva, Eva
Vasileva-Slaveva, Mariela
author_sort Yordanov, Angel
collection PubMed
description Introduction: Cancer of the uterine cervix (CUC) is still one of the most frequent oncological diagnoses in women. The specific interactions between the tumor cells of CUC and the cells and tissues in the tumor microenvironment can affect cancer cells’ invasive and metastatic potential and can modulate tumor’s progression and death. CD47 is a trans-membranous immunoglobulin, expressed in many cells. It protects the cells from being destroyed by the circulating macrophages. Aim: We aimed to evaluate the prognostic role of CD47 expressed in the tumor tissues of patients with CUC for tumor progression and to find the most sensitive immunohistochemical score for defining the cut-off significantly associated with tumor biology and progression. Materials and methods: Paraffin-embedded tumor tissues from 86 patients with CUC were included in the study. Clinico-morphological data for patients, such as age and stage at diagnosis according to FIGO and TNM classification, were obtained from the hospital electronic medical records. Immunohistochemical staining was performed with rabbit recombinant monoclonal CD47 antibody (Clone SP279). The final result was interpreted based on three reporting models in immunohistochemistry: H-score, Allred score and combined score. Results: The expression of CD47 was higher in tumors limited in the cervix compared with those invading other structures, and it did not depend on the nodal status. The results of immunohistochemical staining were similar regardless of which immunohistochemical method was used. The most significant correlation with TNM stage was observed with the H-score (p = 0.00018). The association with the Allred and combined score was less significant, with p values of 0.0013 and 0.0002, respectively. Conclusion: The expression of CD47 in the cancer cells is prognostic for tumor invasion in the surrounding structures, independent of lymph node engagement. The H-score is the most sensitive immunohistochemical score to describe tumor stage. To the best of our knowledge, this is the first study evaluating the significance of CD47 expression in CUC.
format Online
Article
Text
id pubmed-9818840
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98188402023-01-07 Prognostic Utility of CD47 in Cancer of the Uterine Cervix and the Sensitivity of Immunohistochemical Scores Yordanov, Angel Shivarov, Velizar Kostov, Stoyan Ivanova, Yonka Dimitrova, Polina Popovska, Savelina Tsoneva, Eva Vasileva-Slaveva, Mariela Diagnostics (Basel) Article Introduction: Cancer of the uterine cervix (CUC) is still one of the most frequent oncological diagnoses in women. The specific interactions between the tumor cells of CUC and the cells and tissues in the tumor microenvironment can affect cancer cells’ invasive and metastatic potential and can modulate tumor’s progression and death. CD47 is a trans-membranous immunoglobulin, expressed in many cells. It protects the cells from being destroyed by the circulating macrophages. Aim: We aimed to evaluate the prognostic role of CD47 expressed in the tumor tissues of patients with CUC for tumor progression and to find the most sensitive immunohistochemical score for defining the cut-off significantly associated with tumor biology and progression. Materials and methods: Paraffin-embedded tumor tissues from 86 patients with CUC were included in the study. Clinico-morphological data for patients, such as age and stage at diagnosis according to FIGO and TNM classification, were obtained from the hospital electronic medical records. Immunohistochemical staining was performed with rabbit recombinant monoclonal CD47 antibody (Clone SP279). The final result was interpreted based on three reporting models in immunohistochemistry: H-score, Allred score and combined score. Results: The expression of CD47 was higher in tumors limited in the cervix compared with those invading other structures, and it did not depend on the nodal status. The results of immunohistochemical staining were similar regardless of which immunohistochemical method was used. The most significant correlation with TNM stage was observed with the H-score (p = 0.00018). The association with the Allred and combined score was less significant, with p values of 0.0013 and 0.0002, respectively. Conclusion: The expression of CD47 in the cancer cells is prognostic for tumor invasion in the surrounding structures, independent of lymph node engagement. The H-score is the most sensitive immunohistochemical score to describe tumor stage. To the best of our knowledge, this is the first study evaluating the significance of CD47 expression in CUC. MDPI 2022-12-24 /pmc/articles/PMC9818840/ /pubmed/36611344 http://dx.doi.org/10.3390/diagnostics13010052 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yordanov, Angel
Shivarov, Velizar
Kostov, Stoyan
Ivanova, Yonka
Dimitrova, Polina
Popovska, Savelina
Tsoneva, Eva
Vasileva-Slaveva, Mariela
Prognostic Utility of CD47 in Cancer of the Uterine Cervix and the Sensitivity of Immunohistochemical Scores
title Prognostic Utility of CD47 in Cancer of the Uterine Cervix and the Sensitivity of Immunohistochemical Scores
title_full Prognostic Utility of CD47 in Cancer of the Uterine Cervix and the Sensitivity of Immunohistochemical Scores
title_fullStr Prognostic Utility of CD47 in Cancer of the Uterine Cervix and the Sensitivity of Immunohistochemical Scores
title_full_unstemmed Prognostic Utility of CD47 in Cancer of the Uterine Cervix and the Sensitivity of Immunohistochemical Scores
title_short Prognostic Utility of CD47 in Cancer of the Uterine Cervix and the Sensitivity of Immunohistochemical Scores
title_sort prognostic utility of cd47 in cancer of the uterine cervix and the sensitivity of immunohistochemical scores
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818840/
https://www.ncbi.nlm.nih.gov/pubmed/36611344
http://dx.doi.org/10.3390/diagnostics13010052
work_keys_str_mv AT yordanovangel prognosticutilityofcd47incanceroftheuterinecervixandthesensitivityofimmunohistochemicalscores
AT shivarovvelizar prognosticutilityofcd47incanceroftheuterinecervixandthesensitivityofimmunohistochemicalscores
AT kostovstoyan prognosticutilityofcd47incanceroftheuterinecervixandthesensitivityofimmunohistochemicalscores
AT ivanovayonka prognosticutilityofcd47incanceroftheuterinecervixandthesensitivityofimmunohistochemicalscores
AT dimitrovapolina prognosticutilityofcd47incanceroftheuterinecervixandthesensitivityofimmunohistochemicalscores
AT popovskasavelina prognosticutilityofcd47incanceroftheuterinecervixandthesensitivityofimmunohistochemicalscores
AT tsonevaeva prognosticutilityofcd47incanceroftheuterinecervixandthesensitivityofimmunohistochemicalscores
AT vasilevaslavevamariela prognosticutilityofcd47incanceroftheuterinecervixandthesensitivityofimmunohistochemicalscores