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Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy
MicroRNAs (miRNAs) are small noncoding RNAs which mediate some of the pathological mechanisms of diabetic retinopathy. The aim of this study was to identify differentially expressed miRNAs in the vitreal exosomes of proliferative diabetic retinopathy (PDR) patients and non-diabetic controls. Exosome...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818905/ https://www.ncbi.nlm.nih.gov/pubmed/36611916 http://dx.doi.org/10.3390/cells12010123 |
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author | Kot, Agnieszka Kaczmarek, Radoslaw |
author_facet | Kot, Agnieszka Kaczmarek, Radoslaw |
author_sort | Kot, Agnieszka |
collection | PubMed |
description | MicroRNAs (miRNAs) are small noncoding RNAs which mediate some of the pathological mechanisms of diabetic retinopathy. The aim of this study was to identify differentially expressed miRNAs in the vitreal exosomes of proliferative diabetic retinopathy (PDR) patients and non-diabetic controls. Exosomes were extracted from the vitreous samples of 10 PDR patients and 10 controls. The expression of 372 miRNAs was determined using a quantitative polymerase chain reaction (qPCR) panel. We have demonstrated a significant dysregulation in 26 miRNAs. The most remarkable findings include a profound attenuation of the miR-125 family, as well as enhanced miR-21-5p expression in the diabetic samples. We also showed the downregulation of miR-204-5p and the upregulation of let-7g in PDR compared to the controls. This study identified miR-125 and miR-21 as potential targets for further functional analysis regarding their putative role in the pathogenesis of PDR. |
format | Online Article Text |
id | pubmed-9818905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98189052023-01-07 Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy Kot, Agnieszka Kaczmarek, Radoslaw Cells Article MicroRNAs (miRNAs) are small noncoding RNAs which mediate some of the pathological mechanisms of diabetic retinopathy. The aim of this study was to identify differentially expressed miRNAs in the vitreal exosomes of proliferative diabetic retinopathy (PDR) patients and non-diabetic controls. Exosomes were extracted from the vitreous samples of 10 PDR patients and 10 controls. The expression of 372 miRNAs was determined using a quantitative polymerase chain reaction (qPCR) panel. We have demonstrated a significant dysregulation in 26 miRNAs. The most remarkable findings include a profound attenuation of the miR-125 family, as well as enhanced miR-21-5p expression in the diabetic samples. We also showed the downregulation of miR-204-5p and the upregulation of let-7g in PDR compared to the controls. This study identified miR-125 and miR-21 as potential targets for further functional analysis regarding their putative role in the pathogenesis of PDR. MDPI 2022-12-28 /pmc/articles/PMC9818905/ /pubmed/36611916 http://dx.doi.org/10.3390/cells12010123 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kot, Agnieszka Kaczmarek, Radoslaw Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy |
title | Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy |
title_full | Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy |
title_fullStr | Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy |
title_full_unstemmed | Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy |
title_short | Exosomal miRNA Profiling in Vitreous Humor in Proliferative Diabetic Retinopathy |
title_sort | exosomal mirna profiling in vitreous humor in proliferative diabetic retinopathy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818905/ https://www.ncbi.nlm.nih.gov/pubmed/36611916 http://dx.doi.org/10.3390/cells12010123 |
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