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Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal

Birds are potential carriers of pathogens affecting humans and agriculture. Aiming to evaluate the occurrence of the top three most important foodborne pathogens in free-living birds in Portugal, we investigated 108 individual fecal samples from free-living birds and one pooled sample of gull feces...

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Autores principales: Batista, Rita, Saraiva, Margarida, Lopes, Teresa, Silveira, Leonor, Coelho, Anabela, Furtado, Rosália, Castro, Rita, Correia, Cristina Belo, Rodrigues, David, Henriques, Pedro, Lóio, Sara, Soeiro, Vanessa, da Costa, Paulo Martins, Oleastro, Mónica, Pista, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9819048/
https://www.ncbi.nlm.nih.gov/pubmed/36612545
http://dx.doi.org/10.3390/ijerph20010223
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author Batista, Rita
Saraiva, Margarida
Lopes, Teresa
Silveira, Leonor
Coelho, Anabela
Furtado, Rosália
Castro, Rita
Correia, Cristina Belo
Rodrigues, David
Henriques, Pedro
Lóio, Sara
Soeiro, Vanessa
da Costa, Paulo Martins
Oleastro, Mónica
Pista, Angela
author_facet Batista, Rita
Saraiva, Margarida
Lopes, Teresa
Silveira, Leonor
Coelho, Anabela
Furtado, Rosália
Castro, Rita
Correia, Cristina Belo
Rodrigues, David
Henriques, Pedro
Lóio, Sara
Soeiro, Vanessa
da Costa, Paulo Martins
Oleastro, Mónica
Pista, Angela
author_sort Batista, Rita
collection PubMed
description Birds are potential carriers of pathogens affecting humans and agriculture. Aiming to evaluate the occurrence of the top three most important foodborne pathogens in free-living birds in Portugal, we investigated 108 individual fecal samples from free-living birds and one pooled sample of gull feces (n = 50) for the presence of Escherichia coli (pathogenic and non-pathogenic), Salmonella spp. and Campylobacter spp. Virulence- and antimicrobial resistance- (AMR) associated genes were detected by PCR and Whole-Genome Sequencing (WGS), and phenotypic (serotyping and AMR profiles) characterization was performed. Overall, 8.9% of samples tested positive for pathogenic E. coli, 2.8% for Salmonella spp., and 9.9% for Campylobacter spp. AMR was performed on all pathogenic isolates and in a fraction of non-pathogenic E. coli, being detected in 25.9% of them. Ten of the tested E. coli isolates were multidrug-resistant (MDR), and seven of them were Extended-spectrum β-lactamase (ESBL) producers. Among Salmonella (n = 3) and Campylobacter (n = 9), only one strain of C. jejuni was identified as MDR. Most of the identified serotypes/sequence types had already been found to be associated with human disease. These results show that free-living birds in Portugal may act as carriers of foodborne pathogens linked to human disease, some of them resistant to critically important antimicrobials.
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spelling pubmed-98190482023-01-07 Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal Batista, Rita Saraiva, Margarida Lopes, Teresa Silveira, Leonor Coelho, Anabela Furtado, Rosália Castro, Rita Correia, Cristina Belo Rodrigues, David Henriques, Pedro Lóio, Sara Soeiro, Vanessa da Costa, Paulo Martins Oleastro, Mónica Pista, Angela Int J Environ Res Public Health Article Birds are potential carriers of pathogens affecting humans and agriculture. Aiming to evaluate the occurrence of the top three most important foodborne pathogens in free-living birds in Portugal, we investigated 108 individual fecal samples from free-living birds and one pooled sample of gull feces (n = 50) for the presence of Escherichia coli (pathogenic and non-pathogenic), Salmonella spp. and Campylobacter spp. Virulence- and antimicrobial resistance- (AMR) associated genes were detected by PCR and Whole-Genome Sequencing (WGS), and phenotypic (serotyping and AMR profiles) characterization was performed. Overall, 8.9% of samples tested positive for pathogenic E. coli, 2.8% for Salmonella spp., and 9.9% for Campylobacter spp. AMR was performed on all pathogenic isolates and in a fraction of non-pathogenic E. coli, being detected in 25.9% of them. Ten of the tested E. coli isolates were multidrug-resistant (MDR), and seven of them were Extended-spectrum β-lactamase (ESBL) producers. Among Salmonella (n = 3) and Campylobacter (n = 9), only one strain of C. jejuni was identified as MDR. Most of the identified serotypes/sequence types had already been found to be associated with human disease. These results show that free-living birds in Portugal may act as carriers of foodborne pathogens linked to human disease, some of them resistant to critically important antimicrobials. MDPI 2022-12-23 /pmc/articles/PMC9819048/ /pubmed/36612545 http://dx.doi.org/10.3390/ijerph20010223 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Batista, Rita
Saraiva, Margarida
Lopes, Teresa
Silveira, Leonor
Coelho, Anabela
Furtado, Rosália
Castro, Rita
Correia, Cristina Belo
Rodrigues, David
Henriques, Pedro
Lóio, Sara
Soeiro, Vanessa
da Costa, Paulo Martins
Oleastro, Mónica
Pista, Angela
Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal
title Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal
title_full Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal
title_fullStr Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal
title_full_unstemmed Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal
title_short Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli, Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal
title_sort genotypic and phenotypic characterization of pathogenic escherichia coli, salmonella spp., and campylobacter spp., in free-living birds in mainland portugal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9819048/
https://www.ncbi.nlm.nih.gov/pubmed/36612545
http://dx.doi.org/10.3390/ijerph20010223
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