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Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells
Gold nanoparticles (AuNPs) are well known to interact with cells, leading to different cell behaviors such as cell proliferation and differentiation capacity. Biocompatibility and biological functions enhanced by nanomedicine are the most concerning factors in clinical approaches. In the present res...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9819939/ https://www.ncbi.nlm.nih.gov/pubmed/36613448 http://dx.doi.org/10.3390/ijms24010005 |
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author | Lin, Ruei-Hong Lee, Hsu-Tung Yeh, Chun-An Yang, Yi-Chin Shen, Chiung-Chyi Chang, Kai-Bo Liu, Bai-Shuan Hsieh, Hsien-Hsu Wang, Hui-Min David Hung, Huey-Shan |
author_facet | Lin, Ruei-Hong Lee, Hsu-Tung Yeh, Chun-An Yang, Yi-Chin Shen, Chiung-Chyi Chang, Kai-Bo Liu, Bai-Shuan Hsieh, Hsien-Hsu Wang, Hui-Min David Hung, Huey-Shan |
author_sort | Lin, Ruei-Hong |
collection | PubMed |
description | Gold nanoparticles (AuNPs) are well known to interact with cells, leading to different cell behaviors such as cell proliferation and differentiation capacity. Biocompatibility and biological functions enhanced by nanomedicine are the most concerning factors in clinical approaches. In the present research, AuNP solutions were prepared at concentrations of 1.25, 2.5, 5 and 10 ppm for biocompatibility investigations. Ultraviolet–visible spectroscopy was applied to identify the presence of AuNPs under the various concentrations. Dynamic Light Scattering assay was used for the characterization of the size of the AuNPs. The shape of the AuNPs was observed through a Scanning Electron Microscope. Afterward, the mesenchymal stem cells (MSCs) were treated with a differentiation concentration of AuNP solutions in order to measure the biocompatibility of the nanoparticles. Our results demonstrate that AuNPs at 1.25 and 2.5 ppm could significantly enhance MSC proliferation, decrease reactive oxygen species (ROS) generation and attenuate platelet/monocyte activation. Furthermore, the MSC morphology was observed in the presence of filopodia and lamellipodia while being incubated with 1.25 and 2.5 ppm AuNPs, indicating that the adhesion ability was enhanced by the nanoparticles. The expression of matrix metalloproteinase (MMP-2/9) in MSCs was found to be more highly expressed under 1.25 and 2.5 ppm AuNP treatment, relating to better cell migrating ability. Additionally, the cell apoptosis of MSCs investigated with Annexin-V/PI double staining assay and the Fluorescence Activated Cell Sorting (FACS) method demonstrated the lower population of apoptotic cells in 1.25 and 2.5 ppm AuNP treatments, as compared to high concentrations of AuNPs. Additionally, results from a Western blotting assay explored the possibility that the anti-apoptotic proteins Cyclin-D1 and Bcl-2 were remarkably expressed. Meanwhile, real-time PCR analysis demonstrated that the 1.25 and 2.5 ppm AuNP solutions induced a lower expression of inflammatory cytokines (TNF-α, IL-1β, IFN-γ, IL-6 and IL-8). According to the tests performed on an animal model, AuNP 1.25 and 2.5 ppm treatments exhibited the better biocompatibility performance, including anti-inflammation and endothelialization. In brief, 1.25 and 2.5 ppm of AuNP solution was verified to strengthen the biological functions of MSCs, and thus suggests that AuNPs become the biocompatibility nanomedicine for regeneration research. |
format | Online Article Text |
id | pubmed-9819939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98199392023-01-07 Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells Lin, Ruei-Hong Lee, Hsu-Tung Yeh, Chun-An Yang, Yi-Chin Shen, Chiung-Chyi Chang, Kai-Bo Liu, Bai-Shuan Hsieh, Hsien-Hsu Wang, Hui-Min David Hung, Huey-Shan Int J Mol Sci Article Gold nanoparticles (AuNPs) are well known to interact with cells, leading to different cell behaviors such as cell proliferation and differentiation capacity. Biocompatibility and biological functions enhanced by nanomedicine are the most concerning factors in clinical approaches. In the present research, AuNP solutions were prepared at concentrations of 1.25, 2.5, 5 and 10 ppm for biocompatibility investigations. Ultraviolet–visible spectroscopy was applied to identify the presence of AuNPs under the various concentrations. Dynamic Light Scattering assay was used for the characterization of the size of the AuNPs. The shape of the AuNPs was observed through a Scanning Electron Microscope. Afterward, the mesenchymal stem cells (MSCs) were treated with a differentiation concentration of AuNP solutions in order to measure the biocompatibility of the nanoparticles. Our results demonstrate that AuNPs at 1.25 and 2.5 ppm could significantly enhance MSC proliferation, decrease reactive oxygen species (ROS) generation and attenuate platelet/monocyte activation. Furthermore, the MSC morphology was observed in the presence of filopodia and lamellipodia while being incubated with 1.25 and 2.5 ppm AuNPs, indicating that the adhesion ability was enhanced by the nanoparticles. The expression of matrix metalloproteinase (MMP-2/9) in MSCs was found to be more highly expressed under 1.25 and 2.5 ppm AuNP treatment, relating to better cell migrating ability. Additionally, the cell apoptosis of MSCs investigated with Annexin-V/PI double staining assay and the Fluorescence Activated Cell Sorting (FACS) method demonstrated the lower population of apoptotic cells in 1.25 and 2.5 ppm AuNP treatments, as compared to high concentrations of AuNPs. Additionally, results from a Western blotting assay explored the possibility that the anti-apoptotic proteins Cyclin-D1 and Bcl-2 were remarkably expressed. Meanwhile, real-time PCR analysis demonstrated that the 1.25 and 2.5 ppm AuNP solutions induced a lower expression of inflammatory cytokines (TNF-α, IL-1β, IFN-γ, IL-6 and IL-8). According to the tests performed on an animal model, AuNP 1.25 and 2.5 ppm treatments exhibited the better biocompatibility performance, including anti-inflammation and endothelialization. In brief, 1.25 and 2.5 ppm of AuNP solution was verified to strengthen the biological functions of MSCs, and thus suggests that AuNPs become the biocompatibility nanomedicine for regeneration research. MDPI 2022-12-20 /pmc/articles/PMC9819939/ /pubmed/36613448 http://dx.doi.org/10.3390/ijms24010005 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lin, Ruei-Hong Lee, Hsu-Tung Yeh, Chun-An Yang, Yi-Chin Shen, Chiung-Chyi Chang, Kai-Bo Liu, Bai-Shuan Hsieh, Hsien-Hsu Wang, Hui-Min David Hung, Huey-Shan Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells |
title | Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells |
title_full | Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells |
title_fullStr | Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells |
title_full_unstemmed | Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells |
title_short | Favorable Biological Performance Regarding the Interaction between Gold Nanoparticles and Mesenchymal Stem Cells |
title_sort | favorable biological performance regarding the interaction between gold nanoparticles and mesenchymal stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9819939/ https://www.ncbi.nlm.nih.gov/pubmed/36613448 http://dx.doi.org/10.3390/ijms24010005 |
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