Cargando…

Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity

The functionality of circulating leukocytes in dairy cows is suppressed after calving, with negative energy balance as a risk factor. Leukocyte transcriptomic profiles were compared separately in 44 multiparous (MP) and 18 primiparous (PP) Holstein–Friesian cows receiving diets differing in concentr...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheng, Zhangrui, McLaughlin, Danielle L., Little, Mark W., Ferris, Conrad, Salavati, Mazdak, Ingvartsen, Klaus L., Crowe, Mark A., Wathes, D. Claire
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820068/
https://www.ncbi.nlm.nih.gov/pubmed/36613482
http://dx.doi.org/10.3390/ijms24010039
_version_ 1784865380255137792
author Cheng, Zhangrui
McLaughlin, Danielle L.
Little, Mark W.
Ferris, Conrad
Salavati, Mazdak
Ingvartsen, Klaus L.
Crowe, Mark A.
Wathes, D. Claire
author_facet Cheng, Zhangrui
McLaughlin, Danielle L.
Little, Mark W.
Ferris, Conrad
Salavati, Mazdak
Ingvartsen, Klaus L.
Crowe, Mark A.
Wathes, D. Claire
author_sort Cheng, Zhangrui
collection PubMed
description The functionality of circulating leukocytes in dairy cows is suppressed after calving, with negative energy balance as a risk factor. Leukocyte transcriptomic profiles were compared separately in 44 multiparous (MP) and 18 primiparous (PP) Holstein–Friesian cows receiving diets differing in concentrate proportion to test whether immune dysfunction could be mitigated by appropriate nutrition. After calving, cows were offered either (1) low concentrate (LC); (2) medium concentrate (MC) or (3) high concentrate (HC) diets with proportions of concentrate to grass silage of 30%:70%, 50%:50% and 70%:30%, respectively. Cow phenotype data collected included circulating metabolites, milk yield and health and fertility records. RNA sequencing of circulating leukocytes at 14 days in milk was performed. The HC diet improved energy balance in both age groups. There were more differentially expressed genes in PP than MP cows (460 vs. 173, HC vs. LC comparison) with few overlaps. The MP cows on the LC diet showed upregulation of the complement and coagulation cascade and innate immune defence mechanisms against pathogens and had a trend of more cases of mastitis and poorer fertility. In contrast, the PP cows on the HC diet showed greater immune responses based on both gene expression and phenotypic data and longer interval of calving to conception. The leukocytes of MP and PP cows therefore responded differentially to the diets between age, nutrient supply and immunity affecting their health and subsequent fertility.
format Online
Article
Text
id pubmed-9820068
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98200682023-01-07 Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity Cheng, Zhangrui McLaughlin, Danielle L. Little, Mark W. Ferris, Conrad Salavati, Mazdak Ingvartsen, Klaus L. Crowe, Mark A. Wathes, D. Claire Int J Mol Sci Article The functionality of circulating leukocytes in dairy cows is suppressed after calving, with negative energy balance as a risk factor. Leukocyte transcriptomic profiles were compared separately in 44 multiparous (MP) and 18 primiparous (PP) Holstein–Friesian cows receiving diets differing in concentrate proportion to test whether immune dysfunction could be mitigated by appropriate nutrition. After calving, cows were offered either (1) low concentrate (LC); (2) medium concentrate (MC) or (3) high concentrate (HC) diets with proportions of concentrate to grass silage of 30%:70%, 50%:50% and 70%:30%, respectively. Cow phenotype data collected included circulating metabolites, milk yield and health and fertility records. RNA sequencing of circulating leukocytes at 14 days in milk was performed. The HC diet improved energy balance in both age groups. There were more differentially expressed genes in PP than MP cows (460 vs. 173, HC vs. LC comparison) with few overlaps. The MP cows on the LC diet showed upregulation of the complement and coagulation cascade and innate immune defence mechanisms against pathogens and had a trend of more cases of mastitis and poorer fertility. In contrast, the PP cows on the HC diet showed greater immune responses based on both gene expression and phenotypic data and longer interval of calving to conception. The leukocytes of MP and PP cows therefore responded differentially to the diets between age, nutrient supply and immunity affecting their health and subsequent fertility. MDPI 2022-12-20 /pmc/articles/PMC9820068/ /pubmed/36613482 http://dx.doi.org/10.3390/ijms24010039 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cheng, Zhangrui
McLaughlin, Danielle L.
Little, Mark W.
Ferris, Conrad
Salavati, Mazdak
Ingvartsen, Klaus L.
Crowe, Mark A.
Wathes, D. Claire
Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity
title Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity
title_full Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity
title_fullStr Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity
title_full_unstemmed Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity
title_short Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity
title_sort proportion of concentrate in the diet of early lactation dairy cows has contrasting effects on circulating leukocyte global transcriptomic profiles, health and fertility according to parity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820068/
https://www.ncbi.nlm.nih.gov/pubmed/36613482
http://dx.doi.org/10.3390/ijms24010039
work_keys_str_mv AT chengzhangrui proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity
AT mclaughlindaniellel proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity
AT littlemarkw proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity
AT ferrisconrad proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity
AT salavatimazdak proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity
AT ingvartsenklausl proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity
AT crowemarka proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity
AT wathesdclaire proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity
AT proportionofconcentrateinthedietofearlylactationdairycowshascontrastingeffectsoncirculatingleukocyteglobaltranscriptomicprofileshealthandfertilityaccordingtoparity