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Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration
In baker’s yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K(+) uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel α subuni...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820094/ https://www.ncbi.nlm.nih.gov/pubmed/36613841 http://dx.doi.org/10.3390/ijms24010398 |
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author | Kulik, Natalia Kale, Deepika Spurna, Karin Shamayeva, Katsiaryna Hauser, Fabian Milic, Sandra Janout, Hannah Zayats, Vasilina Jacak, Jaroslaw Ludwig, Jost |
author_facet | Kulik, Natalia Kale, Deepika Spurna, Karin Shamayeva, Katsiaryna Hauser, Fabian Milic, Sandra Janout, Hannah Zayats, Vasilina Jacak, Jaroslaw Ludwig, Jost |
author_sort | Kulik, Natalia |
collection | PubMed |
description | In baker’s yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K(+) uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel α subunit. Because most K-channels are proteins containing four channel-building α subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling; we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K(+) concentration. |
format | Online Article Text |
id | pubmed-9820094 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98200942023-01-07 Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration Kulik, Natalia Kale, Deepika Spurna, Karin Shamayeva, Katsiaryna Hauser, Fabian Milic, Sandra Janout, Hannah Zayats, Vasilina Jacak, Jaroslaw Ludwig, Jost Int J Mol Sci Article In baker’s yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K(+) uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel α subunit. Because most K-channels are proteins containing four channel-building α subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling; we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K(+) concentration. MDPI 2022-12-26 /pmc/articles/PMC9820094/ /pubmed/36613841 http://dx.doi.org/10.3390/ijms24010398 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kulik, Natalia Kale, Deepika Spurna, Karin Shamayeva, Katsiaryna Hauser, Fabian Milic, Sandra Janout, Hannah Zayats, Vasilina Jacak, Jaroslaw Ludwig, Jost Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration |
title | Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration |
title_full | Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration |
title_fullStr | Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration |
title_full_unstemmed | Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration |
title_short | Dimerisation of the Yeast K(+) Translocation Protein Trk1 Depends on the K(+) Concentration |
title_sort | dimerisation of the yeast k(+) translocation protein trk1 depends on the k(+) concentration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820094/ https://www.ncbi.nlm.nih.gov/pubmed/36613841 http://dx.doi.org/10.3390/ijms24010398 |
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