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Impact on the Clinical Evolution of Patients with COVID-19 Pneumonia and the Participation of the NFE2L2/KEAP1 Polymorphisms in Regulating SARS-CoV-2 Infection
In patients with severe pneumonia due to COVID-19, the deregulation of oxidative stress is present. Nuclear erythroid factor 2 (NRF2) is regulated by KEAP1, and NRF2 regulates the expression of genes such as NFE2L2-KEAP1, which are involved in cellular defense against oxidative stress. In this study...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820269/ https://www.ncbi.nlm.nih.gov/pubmed/36613859 http://dx.doi.org/10.3390/ijms24010415 |
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author | Soto, María Elena Fuentevilla-Álvarez, Giovanny Palacios-Chavarría, Adrián Vázquez, Rafael Ricardo Valdez Herrera-Bello, Héctor Moreno-Castañeda, Lidia Torres-Paz, Yazmín Estela González-Moyotl, Nadia Janet Pérez-Torres, Idalia Aisa-Alvarez, Alfredo Manzano-Pech, Linaloe Pérez-Torres, Israel Huesca-Gómez, Claudia Gamboa, Ricardo |
author_facet | Soto, María Elena Fuentevilla-Álvarez, Giovanny Palacios-Chavarría, Adrián Vázquez, Rafael Ricardo Valdez Herrera-Bello, Héctor Moreno-Castañeda, Lidia Torres-Paz, Yazmín Estela González-Moyotl, Nadia Janet Pérez-Torres, Idalia Aisa-Alvarez, Alfredo Manzano-Pech, Linaloe Pérez-Torres, Israel Huesca-Gómez, Claudia Gamboa, Ricardo |
author_sort | Soto, María Elena |
collection | PubMed |
description | In patients with severe pneumonia due to COVID-19, the deregulation of oxidative stress is present. Nuclear erythroid factor 2 (NRF2) is regulated by KEAP1, and NRF2 regulates the expression of genes such as NFE2L2-KEAP1, which are involved in cellular defense against oxidative stress. In this study, we analyzed the participation of the polymorphisms of NFE2L2 and KEAP1 genes in the mechanisms of damage in lung disease patients with SARS-CoV-2 infection. Patients with COVID-19 and a control group were included. Organ dysfunction was evaluated using SOFA. SARS-CoV-2 infection was confirmed and classified as moderate or severe by ventilatory status and by the Berlin criteria for acute respiratory distress syndrome. SNPs in the gene locus for NFE2L2, rs2364723C>G, and KEAP1, rs9676881A>G, and rs34197572C>T were determined by qPCR. We analyzed 110 individuals with SARS-CoV-2 infection: 51 with severe evolution and 59 with moderate evolution. We also analyzed 111 controls. Significant differences were found for rs2364723 allele G in severe cases vs. controls (p = 0.02); for the rs9676881 allele G in moderate cases vs. controls (p = 0.04); for the rs34197572 allele T in severe cases vs. controls (p = 0.001); and in severe vs. moderate cases (p = 0.004). Our results showed that NFE2L2 rs2364723C>G allele G had a protective effect against severe COVID-19, while KEAP1 rs9676881A>G allele G and rs34197572C>T minor allele T were associated with more aggressive stages of COVID-19. |
format | Online Article Text |
id | pubmed-9820269 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98202692023-01-07 Impact on the Clinical Evolution of Patients with COVID-19 Pneumonia and the Participation of the NFE2L2/KEAP1 Polymorphisms in Regulating SARS-CoV-2 Infection Soto, María Elena Fuentevilla-Álvarez, Giovanny Palacios-Chavarría, Adrián Vázquez, Rafael Ricardo Valdez Herrera-Bello, Héctor Moreno-Castañeda, Lidia Torres-Paz, Yazmín Estela González-Moyotl, Nadia Janet Pérez-Torres, Idalia Aisa-Alvarez, Alfredo Manzano-Pech, Linaloe Pérez-Torres, Israel Huesca-Gómez, Claudia Gamboa, Ricardo Int J Mol Sci Article In patients with severe pneumonia due to COVID-19, the deregulation of oxidative stress is present. Nuclear erythroid factor 2 (NRF2) is regulated by KEAP1, and NRF2 regulates the expression of genes such as NFE2L2-KEAP1, which are involved in cellular defense against oxidative stress. In this study, we analyzed the participation of the polymorphisms of NFE2L2 and KEAP1 genes in the mechanisms of damage in lung disease patients with SARS-CoV-2 infection. Patients with COVID-19 and a control group were included. Organ dysfunction was evaluated using SOFA. SARS-CoV-2 infection was confirmed and classified as moderate or severe by ventilatory status and by the Berlin criteria for acute respiratory distress syndrome. SNPs in the gene locus for NFE2L2, rs2364723C>G, and KEAP1, rs9676881A>G, and rs34197572C>T were determined by qPCR. We analyzed 110 individuals with SARS-CoV-2 infection: 51 with severe evolution and 59 with moderate evolution. We also analyzed 111 controls. Significant differences were found for rs2364723 allele G in severe cases vs. controls (p = 0.02); for the rs9676881 allele G in moderate cases vs. controls (p = 0.04); for the rs34197572 allele T in severe cases vs. controls (p = 0.001); and in severe vs. moderate cases (p = 0.004). Our results showed that NFE2L2 rs2364723C>G allele G had a protective effect against severe COVID-19, while KEAP1 rs9676881A>G allele G and rs34197572C>T minor allele T were associated with more aggressive stages of COVID-19. MDPI 2022-12-27 /pmc/articles/PMC9820269/ /pubmed/36613859 http://dx.doi.org/10.3390/ijms24010415 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Soto, María Elena Fuentevilla-Álvarez, Giovanny Palacios-Chavarría, Adrián Vázquez, Rafael Ricardo Valdez Herrera-Bello, Héctor Moreno-Castañeda, Lidia Torres-Paz, Yazmín Estela González-Moyotl, Nadia Janet Pérez-Torres, Idalia Aisa-Alvarez, Alfredo Manzano-Pech, Linaloe Pérez-Torres, Israel Huesca-Gómez, Claudia Gamboa, Ricardo Impact on the Clinical Evolution of Patients with COVID-19 Pneumonia and the Participation of the NFE2L2/KEAP1 Polymorphisms in Regulating SARS-CoV-2 Infection |
title | Impact on the Clinical Evolution of Patients with COVID-19 Pneumonia and the Participation of the NFE2L2/KEAP1 Polymorphisms in Regulating SARS-CoV-2 Infection |
title_full | Impact on the Clinical Evolution of Patients with COVID-19 Pneumonia and the Participation of the NFE2L2/KEAP1 Polymorphisms in Regulating SARS-CoV-2 Infection |
title_fullStr | Impact on the Clinical Evolution of Patients with COVID-19 Pneumonia and the Participation of the NFE2L2/KEAP1 Polymorphisms in Regulating SARS-CoV-2 Infection |
title_full_unstemmed | Impact on the Clinical Evolution of Patients with COVID-19 Pneumonia and the Participation of the NFE2L2/KEAP1 Polymorphisms in Regulating SARS-CoV-2 Infection |
title_short | Impact on the Clinical Evolution of Patients with COVID-19 Pneumonia and the Participation of the NFE2L2/KEAP1 Polymorphisms in Regulating SARS-CoV-2 Infection |
title_sort | impact on the clinical evolution of patients with covid-19 pneumonia and the participation of the nfe2l2/keap1 polymorphisms in regulating sars-cov-2 infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820269/ https://www.ncbi.nlm.nih.gov/pubmed/36613859 http://dx.doi.org/10.3390/ijms24010415 |
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