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New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins

Olive oil phenols (OOPs) are associated with the prevention of many human cancers. Some of these have been shown to inhibit cell proliferation and induce apoptosis. However, no systematic comparative study exists for all the investigated compounds under the same conditions, due to difficulties in th...

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Autores principales: Papakonstantinou, Aikaterini, Koumarianou, Petrina, Rigakou, Aimilia, Diamantakos, Panagiotis, Frakolaki, Efseveia, Vassilaki, Niki, Chavdoula, Evangelia, Melliou, Eleni, Magiatis, Prokopios, Boleti, Haralabia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820430/
https://www.ncbi.nlm.nih.gov/pubmed/36613449
http://dx.doi.org/10.3390/ijms24010003
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author Papakonstantinou, Aikaterini
Koumarianou, Petrina
Rigakou, Aimilia
Diamantakos, Panagiotis
Frakolaki, Efseveia
Vassilaki, Niki
Chavdoula, Evangelia
Melliou, Eleni
Magiatis, Prokopios
Boleti, Haralabia
author_facet Papakonstantinou, Aikaterini
Koumarianou, Petrina
Rigakou, Aimilia
Diamantakos, Panagiotis
Frakolaki, Efseveia
Vassilaki, Niki
Chavdoula, Evangelia
Melliou, Eleni
Magiatis, Prokopios
Boleti, Haralabia
author_sort Papakonstantinou, Aikaterini
collection PubMed
description Olive oil phenols (OOPs) are associated with the prevention of many human cancers. Some of these have been shown to inhibit cell proliferation and induce apoptosis. However, no systematic comparative study exists for all the investigated compounds under the same conditions, due to difficulties in their isolation or synthesis. Herein are presented innovative methods for large-scale selective extraction of six major secoiridoids from olive oil or leaves enabling their detailed investigation. The cytotoxic/antiproliferative bioactivity of these six compounds was evaluated on sixteen human cancer cell lines originating from eight different tissues. Cell viability with half-maximal effective concentrations (EC(50)) was evaluated after 72 h treatments. Antiproliferative and pro-apoptotic effects were also assessed for the most bioactive compounds (EC(50) ≤ 50 μM). Oleocanthal (1) showed the strongest antiproliferative/cytotoxic activity in most cancer cell lines (EC(50): 9–20 μM). The relative effectiveness of the six OOPs was: oleocanthal (1) > oleuropein aglycone (3a,b) > ligstroside aglycone (4a,b) > oleacein (2) > oleomissional (6a,b,c) > oleocanthalic acid (7). This is the first detailed study comparing the bioactivity of six OOPs in such a wide array of cancer cell lines, providing a reference for their relative antiproliferative/cytotoxic effect in the investigated cancers.
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spelling pubmed-98204302023-01-07 New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins Papakonstantinou, Aikaterini Koumarianou, Petrina Rigakou, Aimilia Diamantakos, Panagiotis Frakolaki, Efseveia Vassilaki, Niki Chavdoula, Evangelia Melliou, Eleni Magiatis, Prokopios Boleti, Haralabia Int J Mol Sci Article Olive oil phenols (OOPs) are associated with the prevention of many human cancers. Some of these have been shown to inhibit cell proliferation and induce apoptosis. However, no systematic comparative study exists for all the investigated compounds under the same conditions, due to difficulties in their isolation or synthesis. Herein are presented innovative methods for large-scale selective extraction of six major secoiridoids from olive oil or leaves enabling their detailed investigation. The cytotoxic/antiproliferative bioactivity of these six compounds was evaluated on sixteen human cancer cell lines originating from eight different tissues. Cell viability with half-maximal effective concentrations (EC(50)) was evaluated after 72 h treatments. Antiproliferative and pro-apoptotic effects were also assessed for the most bioactive compounds (EC(50) ≤ 50 μM). Oleocanthal (1) showed the strongest antiproliferative/cytotoxic activity in most cancer cell lines (EC(50): 9–20 μM). The relative effectiveness of the six OOPs was: oleocanthal (1) > oleuropein aglycone (3a,b) > ligstroside aglycone (4a,b) > oleacein (2) > oleomissional (6a,b,c) > oleocanthalic acid (7). This is the first detailed study comparing the bioactivity of six OOPs in such a wide array of cancer cell lines, providing a reference for their relative antiproliferative/cytotoxic effect in the investigated cancers. MDPI 2022-12-20 /pmc/articles/PMC9820430/ /pubmed/36613449 http://dx.doi.org/10.3390/ijms24010003 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Papakonstantinou, Aikaterini
Koumarianou, Petrina
Rigakou, Aimilia
Diamantakos, Panagiotis
Frakolaki, Efseveia
Vassilaki, Niki
Chavdoula, Evangelia
Melliou, Eleni
Magiatis, Prokopios
Boleti, Haralabia
New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins
title New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins
title_full New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins
title_fullStr New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins
title_full_unstemmed New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins
title_short New Affordable Methods for Large-Scale Isolation of Major Olive Secoiridoids and Systematic Comparative Study of Their Antiproliferative/Cytotoxic Effect on Multiple Cancer Cell Lines of Different Cancer Origins
title_sort new affordable methods for large-scale isolation of major olive secoiridoids and systematic comparative study of their antiproliferative/cytotoxic effect on multiple cancer cell lines of different cancer origins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820430/
https://www.ncbi.nlm.nih.gov/pubmed/36613449
http://dx.doi.org/10.3390/ijms24010003
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