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Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate

Background: C-X-C motif chemokine ligand 13 (CXCL13), a member of the CXC subtype in chemokine superfamily, affects numerous biological processes of various types of cells and the progress of a great number of clinical diseases. The purpose of the current study was to reveal the internal mechanism b...

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Autores principales: Liu, Daoquan, Li, Mingzhou, Fu, Xun, Yang, Shu, Wang, Zhen, Liu, Jianmin, Li, Yan, Zhou, Yongying, Ren, Pengfei, Guo, Yuhang, Wang, Xinghuan, DiSanto, Michael E., Chen, Ping, Zhang, Xinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820459/
https://www.ncbi.nlm.nih.gov/pubmed/36613500
http://dx.doi.org/10.3390/ijms24010056
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author Liu, Daoquan
Li, Mingzhou
Fu, Xun
Yang, Shu
Wang, Zhen
Liu, Jianmin
Li, Yan
Zhou, Yongying
Ren, Pengfei
Guo, Yuhang
Wang, Xinghuan
DiSanto, Michael E.
Chen, Ping
Zhang, Xinhua
author_facet Liu, Daoquan
Li, Mingzhou
Fu, Xun
Yang, Shu
Wang, Zhen
Liu, Jianmin
Li, Yan
Zhou, Yongying
Ren, Pengfei
Guo, Yuhang
Wang, Xinghuan
DiSanto, Michael E.
Chen, Ping
Zhang, Xinhua
author_sort Liu, Daoquan
collection PubMed
description Background: C-X-C motif chemokine ligand 13 (CXCL13), a member of the CXC subtype in chemokine superfamily, affects numerous biological processes of various types of cells and the progress of a great number of clinical diseases. The purpose of the current study was to reveal the internal mechanism between CXCL13 and benign prostatic hyperplasia (BPH). Methods: Human serum, prostate tissues and human prostate cell lines (BPH-1, WPMY-1) were utilized. The effect of recombinant human CXCL13 (rHuCXCL13) protein and the influences of the knockdown/overexpression of CXCL13 on two cell lines were studied. Rescue experiments by anti-CXCR5 were also conducted. In vivo, rHuCXCL13 was injected into the ventral prostate of rats. Additionally, a tissue microarray of hyperplastic prostate tissues was constructed to analyze the correlations between CXCL13 and clinical parameters. Results: CXCL13 was highly expressed in the prostate tissues and upregulated in the BPH group. It was observed that CXCL13 modulated cell proliferation, apoptosis, and the epithelial–mesenchymal transition (EMT) through CXCR5 via AKT and the ERK1/2 pathway in BPH-1, while it contributed to inflammation and fibrosis through CXCR5 via the STAT3 pathway in WPMY-1. In vivo, rHuCXCL13 induced the development of rat BPH. Additionally, CXCL13 was positively correlated with the prostate volume and total prostate specific antigen. Conclusions: Our novel data demonstrated that CXCL13 modulated cell proliferation, cell cycle, the EMT of epithelial cells, and induced the fibrosis of prostatic stromal cells via a variety of inflammatory factors, suggesting that CXCL13 might be rediscovered as a potential therapeutic target for the treatment of BPH.
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spelling pubmed-98204592023-01-07 Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate Liu, Daoquan Li, Mingzhou Fu, Xun Yang, Shu Wang, Zhen Liu, Jianmin Li, Yan Zhou, Yongying Ren, Pengfei Guo, Yuhang Wang, Xinghuan DiSanto, Michael E. Chen, Ping Zhang, Xinhua Int J Mol Sci Article Background: C-X-C motif chemokine ligand 13 (CXCL13), a member of the CXC subtype in chemokine superfamily, affects numerous biological processes of various types of cells and the progress of a great number of clinical diseases. The purpose of the current study was to reveal the internal mechanism between CXCL13 and benign prostatic hyperplasia (BPH). Methods: Human serum, prostate tissues and human prostate cell lines (BPH-1, WPMY-1) were utilized. The effect of recombinant human CXCL13 (rHuCXCL13) protein and the influences of the knockdown/overexpression of CXCL13 on two cell lines were studied. Rescue experiments by anti-CXCR5 were also conducted. In vivo, rHuCXCL13 was injected into the ventral prostate of rats. Additionally, a tissue microarray of hyperplastic prostate tissues was constructed to analyze the correlations between CXCL13 and clinical parameters. Results: CXCL13 was highly expressed in the prostate tissues and upregulated in the BPH group. It was observed that CXCL13 modulated cell proliferation, apoptosis, and the epithelial–mesenchymal transition (EMT) through CXCR5 via AKT and the ERK1/2 pathway in BPH-1, while it contributed to inflammation and fibrosis through CXCR5 via the STAT3 pathway in WPMY-1. In vivo, rHuCXCL13 induced the development of rat BPH. Additionally, CXCL13 was positively correlated with the prostate volume and total prostate specific antigen. Conclusions: Our novel data demonstrated that CXCL13 modulated cell proliferation, cell cycle, the EMT of epithelial cells, and induced the fibrosis of prostatic stromal cells via a variety of inflammatory factors, suggesting that CXCL13 might be rediscovered as a potential therapeutic target for the treatment of BPH. MDPI 2022-12-21 /pmc/articles/PMC9820459/ /pubmed/36613500 http://dx.doi.org/10.3390/ijms24010056 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Daoquan
Li, Mingzhou
Fu, Xun
Yang, Shu
Wang, Zhen
Liu, Jianmin
Li, Yan
Zhou, Yongying
Ren, Pengfei
Guo, Yuhang
Wang, Xinghuan
DiSanto, Michael E.
Chen, Ping
Zhang, Xinhua
Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate
title Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate
title_full Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate
title_fullStr Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate
title_full_unstemmed Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate
title_short Changes in the Expression and Functional Activities of C-X-C Motif Chemokine Ligand 13 (CXCL13) in Hyperplastic Prostate
title_sort changes in the expression and functional activities of c-x-c motif chemokine ligand 13 (cxcl13) in hyperplastic prostate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820459/
https://www.ncbi.nlm.nih.gov/pubmed/36613500
http://dx.doi.org/10.3390/ijms24010056
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