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A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface

Preparing biological specimens for scanning electron microscopy (SEM) can be difficult to implement, as it requires specialized equipment and materials as well as the training of dedicated personnel. Moreover, the procedure often results in damage to the samples to be analyzed. This work presents a...

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Detalles Bibliográficos
Autores principales: Minuti, Anca Emanuela, Labusca, Luminita, Herea, Dumitru-Daniel, Stoian, George, Chiriac, Horia, Lupu, Nicoleta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820490/
https://www.ncbi.nlm.nih.gov/pubmed/36613905
http://dx.doi.org/10.3390/ijms24010430
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author Minuti, Anca Emanuela
Labusca, Luminita
Herea, Dumitru-Daniel
Stoian, George
Chiriac, Horia
Lupu, Nicoleta
author_facet Minuti, Anca Emanuela
Labusca, Luminita
Herea, Dumitru-Daniel
Stoian, George
Chiriac, Horia
Lupu, Nicoleta
author_sort Minuti, Anca Emanuela
collection PubMed
description Preparing biological specimens for scanning electron microscopy (SEM) can be difficult to implement, as it requires specialized equipment and materials as well as the training of dedicated personnel. Moreover, the procedure often results in damage to the samples to be analyzed. This work presents a protocol for the preparation of biological samples to evaluate the adherence of nanomaterials on the cell surface using SEM. To this end, we used silicon wafers as a substrate to grow cells and replaced difficult steps such as the critical point drying of the samples in order to make the method quicker and easier to perform. The new protocol was tested using two different types of cells, i.e., human osteosarcoma cells and adipose-derived mesenchymal stem cells, and it proved that it can grossly preserve cell integrity in order to be used to estimate nanomaterials’ interaction with cell surfaces.
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spelling pubmed-98204902023-01-07 A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface Minuti, Anca Emanuela Labusca, Luminita Herea, Dumitru-Daniel Stoian, George Chiriac, Horia Lupu, Nicoleta Int J Mol Sci Article Preparing biological specimens for scanning electron microscopy (SEM) can be difficult to implement, as it requires specialized equipment and materials as well as the training of dedicated personnel. Moreover, the procedure often results in damage to the samples to be analyzed. This work presents a protocol for the preparation of biological samples to evaluate the adherence of nanomaterials on the cell surface using SEM. To this end, we used silicon wafers as a substrate to grow cells and replaced difficult steps such as the critical point drying of the samples in order to make the method quicker and easier to perform. The new protocol was tested using two different types of cells, i.e., human osteosarcoma cells and adipose-derived mesenchymal stem cells, and it proved that it can grossly preserve cell integrity in order to be used to estimate nanomaterials’ interaction with cell surfaces. MDPI 2022-12-27 /pmc/articles/PMC9820490/ /pubmed/36613905 http://dx.doi.org/10.3390/ijms24010430 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Minuti, Anca Emanuela
Labusca, Luminita
Herea, Dumitru-Daniel
Stoian, George
Chiriac, Horia
Lupu, Nicoleta
A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface
title A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface
title_full A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface
title_fullStr A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface
title_full_unstemmed A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface
title_short A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface
title_sort simple protocol for sample preparation for scanning electron microscopic imaging allows quick screening of nanomaterials adhering to cell surface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820490/
https://www.ncbi.nlm.nih.gov/pubmed/36613905
http://dx.doi.org/10.3390/ijms24010430
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