Cargando…
RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle
Meat quality has a close relationship with fat and connective tissue; therefore, screening and identifying functional genes related to lipid metabolism is essential for the production of high-grade beef. The transcriptomes of the Longissimus dorsi muscle in Wagyu and Chinese Red Steppe cattle, breed...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820533/ https://www.ncbi.nlm.nih.gov/pubmed/36613828 http://dx.doi.org/10.3390/ijms24010387 |
_version_ | 1784865487079866368 |
---|---|
author | Li, Guanghui Yang, Runjun Lu, Xin Liu, Yue He, Wei Li, Yue Yu, Haibin Qin, Lihong Cao, Yang Zhao, Zhihui Fang, Xibi |
author_facet | Li, Guanghui Yang, Runjun Lu, Xin Liu, Yue He, Wei Li, Yue Yu, Haibin Qin, Lihong Cao, Yang Zhao, Zhihui Fang, Xibi |
author_sort | Li, Guanghui |
collection | PubMed |
description | Meat quality has a close relationship with fat and connective tissue; therefore, screening and identifying functional genes related to lipid metabolism is essential for the production of high-grade beef. The transcriptomes of the Longissimus dorsi muscle in Wagyu and Chinese Red Steppe cattle, breeds with significant differences in meat quality and intramuscular fat deposition, were analyzed using RNA-seq to screen for candidate genes associated with beef quality traits. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the 388 differentially expressed genes (DEGs) were involved in biological processes such as short-chain fatty acid metabolism, regulation of fatty acid transport and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. In addition, crystallin alpha B (CRYAB), ankyrin repeat domain 2 (ANKRD2), aldehyde dehydrogenase 9 family member A1 (ALDH9A1) and enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase (EHHADH) were investigated for their effects on intracellular triglyceride and fatty acid content and their regulatory effects on genes in lipogenesis and fatty acid metabolism pathways. This study generated a dataset from transcriptome profiling of two cattle breeds, with differing capacities for fat-deposition in the muscle, and revealed molecular evidence that CRYAB, ANKRD2, ALDH9A1 and EHHADH are related to fat metabolism in bovine fetal fibroblasts (BFFs). The results provide potential functional genes for maker-assisted selection and molecular breeding to improve meat quality traits in beef cattle. |
format | Online Article Text |
id | pubmed-9820533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98205332023-01-07 RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle Li, Guanghui Yang, Runjun Lu, Xin Liu, Yue He, Wei Li, Yue Yu, Haibin Qin, Lihong Cao, Yang Zhao, Zhihui Fang, Xibi Int J Mol Sci Article Meat quality has a close relationship with fat and connective tissue; therefore, screening and identifying functional genes related to lipid metabolism is essential for the production of high-grade beef. The transcriptomes of the Longissimus dorsi muscle in Wagyu and Chinese Red Steppe cattle, breeds with significant differences in meat quality and intramuscular fat deposition, were analyzed using RNA-seq to screen for candidate genes associated with beef quality traits. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the 388 differentially expressed genes (DEGs) were involved in biological processes such as short-chain fatty acid metabolism, regulation of fatty acid transport and the peroxisome proliferator-activated receptor (PPAR) signaling pathway. In addition, crystallin alpha B (CRYAB), ankyrin repeat domain 2 (ANKRD2), aldehyde dehydrogenase 9 family member A1 (ALDH9A1) and enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase (EHHADH) were investigated for their effects on intracellular triglyceride and fatty acid content and their regulatory effects on genes in lipogenesis and fatty acid metabolism pathways. This study generated a dataset from transcriptome profiling of two cattle breeds, with differing capacities for fat-deposition in the muscle, and revealed molecular evidence that CRYAB, ANKRD2, ALDH9A1 and EHHADH are related to fat metabolism in bovine fetal fibroblasts (BFFs). The results provide potential functional genes for maker-assisted selection and molecular breeding to improve meat quality traits in beef cattle. MDPI 2022-12-26 /pmc/articles/PMC9820533/ /pubmed/36613828 http://dx.doi.org/10.3390/ijms24010387 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Guanghui Yang, Runjun Lu, Xin Liu, Yue He, Wei Li, Yue Yu, Haibin Qin, Lihong Cao, Yang Zhao, Zhihui Fang, Xibi RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle |
title | RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle |
title_full | RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle |
title_fullStr | RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle |
title_full_unstemmed | RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle |
title_short | RNA-Seq Analysis Identifies Differentially Expressed Genes in the Longissimus dorsi of Wagyu and Chinese Red Steppe Cattle |
title_sort | rna-seq analysis identifies differentially expressed genes in the longissimus dorsi of wagyu and chinese red steppe cattle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820533/ https://www.ncbi.nlm.nih.gov/pubmed/36613828 http://dx.doi.org/10.3390/ijms24010387 |
work_keys_str_mv | AT liguanghui rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT yangrunjun rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT luxin rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT liuyue rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT hewei rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT liyue rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT yuhaibin rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT qinlihong rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT caoyang rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT zhaozhihui rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle AT fangxibi rnaseqanalysisidentifiesdifferentiallyexpressedgenesinthelongissimusdorsiofwagyuandchineseredsteppecattle |