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Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus

The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genot...

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Autores principales: Flasz, Barbara, Dziewięcka, Marta, Ajay, Amrendra K., Tarnawska, Monika, Babczyńska, Agnieszka, Kędziorski, Andrzej, Napora-Rutkowski, Łukasz, Ziętara, Patrycja, Świerczek, Ewa, Augustyniak, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820743/
https://www.ncbi.nlm.nih.gov/pubmed/36613733
http://dx.doi.org/10.3390/ijms24010290
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author Flasz, Barbara
Dziewięcka, Marta
Ajay, Amrendra K.
Tarnawska, Monika
Babczyńska, Agnieszka
Kędziorski, Andrzej
Napora-Rutkowski, Łukasz
Ziętara, Patrycja
Świerczek, Ewa
Augustyniak, Maria
author_facet Flasz, Barbara
Dziewięcka, Marta
Ajay, Amrendra K.
Tarnawska, Monika
Babczyńska, Agnieszka
Kędziorski, Andrzej
Napora-Rutkowski, Łukasz
Ziętara, Patrycja
Świerczek, Ewa
Augustyniak, Maria
author_sort Flasz, Barbara
collection PubMed
description The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks—DSB, 8-hydroxy-2′-deoxyguanosine-8-OHdG, abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms.
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spelling pubmed-98207432023-01-07 Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus Flasz, Barbara Dziewięcka, Marta Ajay, Amrendra K. Tarnawska, Monika Babczyńska, Agnieszka Kędziorski, Andrzej Napora-Rutkowski, Łukasz Ziętara, Patrycja Świerczek, Ewa Augustyniak, Maria Int J Mol Sci Article The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks—DSB, 8-hydroxy-2′-deoxyguanosine-8-OHdG, abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms. MDPI 2022-12-24 /pmc/articles/PMC9820743/ /pubmed/36613733 http://dx.doi.org/10.3390/ijms24010290 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Flasz, Barbara
Dziewięcka, Marta
Ajay, Amrendra K.
Tarnawska, Monika
Babczyńska, Agnieszka
Kędziorski, Andrzej
Napora-Rutkowski, Łukasz
Ziętara, Patrycja
Świerczek, Ewa
Augustyniak, Maria
Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus
title Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus
title_full Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus
title_fullStr Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus
title_full_unstemmed Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus
title_short Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus
title_sort age- and lifespan-dependent differences in go caused dna damage in acheta domesticus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820743/
https://www.ncbi.nlm.nih.gov/pubmed/36613733
http://dx.doi.org/10.3390/ijms24010290
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