Cargando…
Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus
The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genot...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820743/ https://www.ncbi.nlm.nih.gov/pubmed/36613733 http://dx.doi.org/10.3390/ijms24010290 |
_version_ | 1784865534984060928 |
---|---|
author | Flasz, Barbara Dziewięcka, Marta Ajay, Amrendra K. Tarnawska, Monika Babczyńska, Agnieszka Kędziorski, Andrzej Napora-Rutkowski, Łukasz Ziętara, Patrycja Świerczek, Ewa Augustyniak, Maria |
author_facet | Flasz, Barbara Dziewięcka, Marta Ajay, Amrendra K. Tarnawska, Monika Babczyńska, Agnieszka Kędziorski, Andrzej Napora-Rutkowski, Łukasz Ziętara, Patrycja Świerczek, Ewa Augustyniak, Maria |
author_sort | Flasz, Barbara |
collection | PubMed |
description | The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks—DSB, 8-hydroxy-2′-deoxyguanosine-8-OHdG, abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms. |
format | Online Article Text |
id | pubmed-9820743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98207432023-01-07 Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus Flasz, Barbara Dziewięcka, Marta Ajay, Amrendra K. Tarnawska, Monika Babczyńska, Agnieszka Kędziorski, Andrzej Napora-Rutkowski, Łukasz Ziętara, Patrycja Świerczek, Ewa Augustyniak, Maria Int J Mol Sci Article The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and lack of toxicity. Sensitivity to GO of immature, or with different survival strategy, individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (total DNA damage, double-strand breaks—DSB, 8-hydroxy-2′-deoxyguanosine-8-OHdG, abasic site—AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H), and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms. MDPI 2022-12-24 /pmc/articles/PMC9820743/ /pubmed/36613733 http://dx.doi.org/10.3390/ijms24010290 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Flasz, Barbara Dziewięcka, Marta Ajay, Amrendra K. Tarnawska, Monika Babczyńska, Agnieszka Kędziorski, Andrzej Napora-Rutkowski, Łukasz Ziętara, Patrycja Świerczek, Ewa Augustyniak, Maria Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus |
title | Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus |
title_full | Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus |
title_fullStr | Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus |
title_full_unstemmed | Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus |
title_short | Age- and Lifespan-Dependent Differences in GO Caused DNA Damage in Acheta domesticus |
title_sort | age- and lifespan-dependent differences in go caused dna damage in acheta domesticus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820743/ https://www.ncbi.nlm.nih.gov/pubmed/36613733 http://dx.doi.org/10.3390/ijms24010290 |
work_keys_str_mv | AT flaszbarbara ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT dziewieckamarta ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT ajayamrendrak ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT tarnawskamonika ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT babczynskaagnieszka ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT kedziorskiandrzej ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT naporarutkowskiłukasz ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT zietarapatrycja ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT swierczekewa ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus AT augustyniakmaria ageandlifespandependentdifferencesingocauseddnadamageinachetadomesticus |