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Application of Antimicrobial Photodynamic Therapy for Inactivation of Acinetobacter baumannii Biofilms

Acinetobacter baumannii is a dangerous hospital pathogen primarily due to its ability to form biofilms on different abiotic and biotic surfaces. The present study investigated the effect of riboflavin- and chlorophyllin-based antimicrobial photodynamic therapy, performed with near-ultraviolet or blu...

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Autores principales: Buchovec, Irina, Vyčaitė, Enrika, Badokas, Kazimieras, Sužiedelienė, Edita, Bagdonas, Saulius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820809/
https://www.ncbi.nlm.nih.gov/pubmed/36614160
http://dx.doi.org/10.3390/ijms24010722
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author Buchovec, Irina
Vyčaitė, Enrika
Badokas, Kazimieras
Sužiedelienė, Edita
Bagdonas, Saulius
author_facet Buchovec, Irina
Vyčaitė, Enrika
Badokas, Kazimieras
Sužiedelienė, Edita
Bagdonas, Saulius
author_sort Buchovec, Irina
collection PubMed
description Acinetobacter baumannii is a dangerous hospital pathogen primarily due to its ability to form biofilms on different abiotic and biotic surfaces. The present study investigated the effect of riboflavin- and chlorophyllin-based antimicrobial photodynamic therapy, performed with near-ultraviolet or blue light on the viability of bacterial cells in biofilms and their structural stability, also determining the extent of photoinduced generation of intracellular reactive oxygen species as well as the ability of A. baumannii to form biofilms after the treatment. The efficacy of antimicrobial photodynamic therapy was compared with that of light alone and the role of the photosensitizer type on the photosensitization mechanism was demonstrated. We found that the antibacterial effect of riboflavin-based antimicrobial photodynamic therapy depends on the ability of photoactivated riboflavin to generate intracellular reactive oxygen species but does not depend on the concentration of riboflavin and pre-incubation time before irradiation. Moreover, our results suggest a clear interconnection between the inactivation efficiency of chlorophyllin-based antimicrobial photodynamic therapy and the sensitivity of A. baumannii biofilms to used light. In summary, all the analyzed results suggest that riboflavin-based antimicrobial photodynamic therapy and chlorophyllin-based antimicrobial photodynamic therapy have the potential to be applied as an antibacterial treatment against A. baumannii biofilms or as a preventive measure against biofilm formation.
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spelling pubmed-98208092023-01-07 Application of Antimicrobial Photodynamic Therapy for Inactivation of Acinetobacter baumannii Biofilms Buchovec, Irina Vyčaitė, Enrika Badokas, Kazimieras Sužiedelienė, Edita Bagdonas, Saulius Int J Mol Sci Article Acinetobacter baumannii is a dangerous hospital pathogen primarily due to its ability to form biofilms on different abiotic and biotic surfaces. The present study investigated the effect of riboflavin- and chlorophyllin-based antimicrobial photodynamic therapy, performed with near-ultraviolet or blue light on the viability of bacterial cells in biofilms and their structural stability, also determining the extent of photoinduced generation of intracellular reactive oxygen species as well as the ability of A. baumannii to form biofilms after the treatment. The efficacy of antimicrobial photodynamic therapy was compared with that of light alone and the role of the photosensitizer type on the photosensitization mechanism was demonstrated. We found that the antibacterial effect of riboflavin-based antimicrobial photodynamic therapy depends on the ability of photoactivated riboflavin to generate intracellular reactive oxygen species but does not depend on the concentration of riboflavin and pre-incubation time before irradiation. Moreover, our results suggest a clear interconnection between the inactivation efficiency of chlorophyllin-based antimicrobial photodynamic therapy and the sensitivity of A. baumannii biofilms to used light. In summary, all the analyzed results suggest that riboflavin-based antimicrobial photodynamic therapy and chlorophyllin-based antimicrobial photodynamic therapy have the potential to be applied as an antibacterial treatment against A. baumannii biofilms or as a preventive measure against biofilm formation. MDPI 2022-12-31 /pmc/articles/PMC9820809/ /pubmed/36614160 http://dx.doi.org/10.3390/ijms24010722 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Buchovec, Irina
Vyčaitė, Enrika
Badokas, Kazimieras
Sužiedelienė, Edita
Bagdonas, Saulius
Application of Antimicrobial Photodynamic Therapy for Inactivation of Acinetobacter baumannii Biofilms
title Application of Antimicrobial Photodynamic Therapy for Inactivation of Acinetobacter baumannii Biofilms
title_full Application of Antimicrobial Photodynamic Therapy for Inactivation of Acinetobacter baumannii Biofilms
title_fullStr Application of Antimicrobial Photodynamic Therapy for Inactivation of Acinetobacter baumannii Biofilms
title_full_unstemmed Application of Antimicrobial Photodynamic Therapy for Inactivation of Acinetobacter baumannii Biofilms
title_short Application of Antimicrobial Photodynamic Therapy for Inactivation of Acinetobacter baumannii Biofilms
title_sort application of antimicrobial photodynamic therapy for inactivation of acinetobacter baumannii biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820809/
https://www.ncbi.nlm.nih.gov/pubmed/36614160
http://dx.doi.org/10.3390/ijms24010722
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