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G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells

To elucidate the currently unknown molecular mechanisms responsible for the aberrant expression of recoverin (Rec) within cancerous cells, we examined two-dimensional (2D) and three-dimensional (3D) cultures of Rec-negative lung adenocarcinoma A549 cells which had been transfected with a plasmid con...

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Autores principales: Ichioka, Hanae, Hirohashi, Yoshihiko, Sato, Tatsuya, Furuhashi, Masato, Watanabe, Megumi, Ida, Yosuke, Hikage, Fumihito, Torigoe, Toshihiko, Ohguro, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820968/
https://www.ncbi.nlm.nih.gov/pubmed/36614215
http://dx.doi.org/10.3390/ijms24010771
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author Ichioka, Hanae
Hirohashi, Yoshihiko
Sato, Tatsuya
Furuhashi, Masato
Watanabe, Megumi
Ida, Yosuke
Hikage, Fumihito
Torigoe, Toshihiko
Ohguro, Hiroshi
author_facet Ichioka, Hanae
Hirohashi, Yoshihiko
Sato, Tatsuya
Furuhashi, Masato
Watanabe, Megumi
Ida, Yosuke
Hikage, Fumihito
Torigoe, Toshihiko
Ohguro, Hiroshi
author_sort Ichioka, Hanae
collection PubMed
description To elucidate the currently unknown molecular mechanisms responsible for the aberrant expression of recoverin (Rec) within cancerous cells, we examined two-dimensional (2D) and three-dimensional (3D) cultures of Rec-negative lung adenocarcinoma A549 cells which had been transfected with a plasmid containing human recoverin cDNA (A549 Rec) or an empty plasmid as a mock control (A549 MOCK). Using these cells, we measured cytotoxicity by several anti-tumor agents (2D), cellular metabolism including mitochondrial and glycolytic functions by a Seahorse bio-analyzer (2D), the physical properties, size and stiffness of the 3D spheroids, trypsin sensitivities (2D and 3D), and RNA sequencing analysis (2D). Compared with the A549 MOCK, the A549 Rec cells showed (1) more sensitivity toward anti-tumor agents (2D) and a 0.25% solution of trypsin (3D); (2) a metabolic shift from glycolysis to oxidative phosphorylation; and (3) the formation of larger and stiffer 3D spheroids. RNA sequencing analysis and bioinformatic analyses of the differentially expressed genes (DEGs) using Gene Ontology (GO) enrichment analysis suggested that aberrantly expressed Rec is most likely associated with several canonical pathways including G-protein-coupled receptor (GPCR)-mediated signaling and signaling by the cAMP response element binding protein (CREB). The findings reported here indicate that the aberrantly expressed Rec-induced modulation of the cell viability and drug sensitivity may be GPCR mediated.
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spelling pubmed-98209682023-01-07 G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells Ichioka, Hanae Hirohashi, Yoshihiko Sato, Tatsuya Furuhashi, Masato Watanabe, Megumi Ida, Yosuke Hikage, Fumihito Torigoe, Toshihiko Ohguro, Hiroshi Int J Mol Sci Article To elucidate the currently unknown molecular mechanisms responsible for the aberrant expression of recoverin (Rec) within cancerous cells, we examined two-dimensional (2D) and three-dimensional (3D) cultures of Rec-negative lung adenocarcinoma A549 cells which had been transfected with a plasmid containing human recoverin cDNA (A549 Rec) or an empty plasmid as a mock control (A549 MOCK). Using these cells, we measured cytotoxicity by several anti-tumor agents (2D), cellular metabolism including mitochondrial and glycolytic functions by a Seahorse bio-analyzer (2D), the physical properties, size and stiffness of the 3D spheroids, trypsin sensitivities (2D and 3D), and RNA sequencing analysis (2D). Compared with the A549 MOCK, the A549 Rec cells showed (1) more sensitivity toward anti-tumor agents (2D) and a 0.25% solution of trypsin (3D); (2) a metabolic shift from glycolysis to oxidative phosphorylation; and (3) the formation of larger and stiffer 3D spheroids. RNA sequencing analysis and bioinformatic analyses of the differentially expressed genes (DEGs) using Gene Ontology (GO) enrichment analysis suggested that aberrantly expressed Rec is most likely associated with several canonical pathways including G-protein-coupled receptor (GPCR)-mediated signaling and signaling by the cAMP response element binding protein (CREB). The findings reported here indicate that the aberrantly expressed Rec-induced modulation of the cell viability and drug sensitivity may be GPCR mediated. MDPI 2023-01-01 /pmc/articles/PMC9820968/ /pubmed/36614215 http://dx.doi.org/10.3390/ijms24010771 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ichioka, Hanae
Hirohashi, Yoshihiko
Sato, Tatsuya
Furuhashi, Masato
Watanabe, Megumi
Ida, Yosuke
Hikage, Fumihito
Torigoe, Toshihiko
Ohguro, Hiroshi
G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells
title G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells
title_full G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells
title_fullStr G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells
title_full_unstemmed G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells
title_short G-Protein-Coupled Receptors Mediate Modulations of Cell Viability and Drug Sensitivity by Aberrantly Expressed Recoverin 3 within A549 Cells
title_sort g-protein-coupled receptors mediate modulations of cell viability and drug sensitivity by aberrantly expressed recoverin 3 within a549 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9820968/
https://www.ncbi.nlm.nih.gov/pubmed/36614215
http://dx.doi.org/10.3390/ijms24010771
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