microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines

Various microRNAs (miRNAs) present in autologous blood products of canines have not been studied recently. We aimed to elucidate the existence of miRNAs in platelet-rich fibrin (PRF) and the stability of canine autologous blood products under various storage conditions. Total RNAs were isolated from...

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Autores principales: Jasineviciute, Indre, Hasan, Md Nazmul, Grigas, Juozas, Pautienius, Arnoldas, Stankevicius, Arunas, Zymantiene, Judita, Miura, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821218/
https://www.ncbi.nlm.nih.gov/pubmed/36614213
http://dx.doi.org/10.3390/ijms24010770
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author Jasineviciute, Indre
Hasan, Md Nazmul
Grigas, Juozas
Pautienius, Arnoldas
Stankevicius, Arunas
Zymantiene, Judita
Miura, Naoki
author_facet Jasineviciute, Indre
Hasan, Md Nazmul
Grigas, Juozas
Pautienius, Arnoldas
Stankevicius, Arunas
Zymantiene, Judita
Miura, Naoki
author_sort Jasineviciute, Indre
collection PubMed
description Various microRNAs (miRNAs) present in autologous blood products of canines have not been studied recently. We aimed to elucidate the existence of miRNAs in platelet-rich fibrin (PRF) and the stability of canine autologous blood products under various storage conditions. Total RNAs were isolated from PRF and other autologous blood products following newly adapted protocols used in commercial kits for plasma and tissue samples. Quantitative real-time polymerase chain reaction analysis (qPCR) was used to detect miRNAs in autologous blood products. The miR-16, miR-21, miR-155, and miR-146a were abundant in PRF and other autologous blood products of canines. Furthermore, we found they could maintain stability under protracted freezing temperatures of −30 °C for at least one month. Our findings revealed that PRF might be a stable resource for various canine miRNAs.
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spelling pubmed-98212182023-01-07 microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines Jasineviciute, Indre Hasan, Md Nazmul Grigas, Juozas Pautienius, Arnoldas Stankevicius, Arunas Zymantiene, Judita Miura, Naoki Int J Mol Sci Article Various microRNAs (miRNAs) present in autologous blood products of canines have not been studied recently. We aimed to elucidate the existence of miRNAs in platelet-rich fibrin (PRF) and the stability of canine autologous blood products under various storage conditions. Total RNAs were isolated from PRF and other autologous blood products following newly adapted protocols used in commercial kits for plasma and tissue samples. Quantitative real-time polymerase chain reaction analysis (qPCR) was used to detect miRNAs in autologous blood products. The miR-16, miR-21, miR-155, and miR-146a were abundant in PRF and other autologous blood products of canines. Furthermore, we found they could maintain stability under protracted freezing temperatures of −30 °C for at least one month. Our findings revealed that PRF might be a stable resource for various canine miRNAs. MDPI 2023-01-01 /pmc/articles/PMC9821218/ /pubmed/36614213 http://dx.doi.org/10.3390/ijms24010770 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jasineviciute, Indre
Hasan, Md Nazmul
Grigas, Juozas
Pautienius, Arnoldas
Stankevicius, Arunas
Zymantiene, Judita
Miura, Naoki
microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines
title microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines
title_full microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines
title_fullStr microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines
title_full_unstemmed microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines
title_short microRNAs Are Abundant and Stable in Platelet-Rich Fibrin and Other Autologous Blood Products of Canines
title_sort micrornas are abundant and stable in platelet-rich fibrin and other autologous blood products of canines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821218/
https://www.ncbi.nlm.nih.gov/pubmed/36614213
http://dx.doi.org/10.3390/ijms24010770
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