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Semi-Quantitative Method of Assessing the Thrombogenicity of Biomaterials Intended for Long-Term Blood Contact

Biomaterials used in cardiosurgical implants and artificial valves that have long-term contact with blood pose a great challenge for researchers due to the induction of thrombogenicity. So far, the assessment of the thrombogenicity of biomaterials has been performed with the use of highly subjective...

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Autores principales: Gawlikowski, Maciej, Major, Roman, Mika, Barbara, Komorowski, Dariusz, Janiczak, Karolina, Tkacz, Ewaryst, Tamulewicz, Anna, Piaseczna, Natalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821258/
https://www.ncbi.nlm.nih.gov/pubmed/36614378
http://dx.doi.org/10.3390/ma16010038
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author Gawlikowski, Maciej
Major, Roman
Mika, Barbara
Komorowski, Dariusz
Janiczak, Karolina
Tkacz, Ewaryst
Tamulewicz, Anna
Piaseczna, Natalia
author_facet Gawlikowski, Maciej
Major, Roman
Mika, Barbara
Komorowski, Dariusz
Janiczak, Karolina
Tkacz, Ewaryst
Tamulewicz, Anna
Piaseczna, Natalia
author_sort Gawlikowski, Maciej
collection PubMed
description Biomaterials used in cardiosurgical implants and artificial valves that have long-term contact with blood pose a great challenge for researchers due to the induction of thrombogenicity. So far, the assessment of the thrombogenicity of biomaterials has been performed with the use of highly subjective descriptive methods, which has made it impossible to compare the results of various experiments. The aim of this paper was to present a new semi-quantitative method of thrombogenicity assessment based on scanning electron microscope (SEM) images of an adhered biological material deposited on the surfaces of prepared samples. The following biomaterials were used to develop the proposed method: Bionate 55D polyurethane, polyether-ether ketone, Ti6Al7Nb alloy, sintered yttria-stabilized zirconium oxide (ZrO(2) + Y(2)O(3)), collagen-coated glass, and bacterial cellulose. The samples were prepared by incubating the biomaterials with platelet-rich plasma. In order to quantify the thrombogenic properties of the biomaterials, a TR parameter based on the fractal dimension was applied. The obtained results confirmed that the use of the fractal dimension enables the quantitative assessment of thrombogenicity and the proper qualification of samples in line with an expert’s judgment. The polyurethanes showed the best thrombogenic properties of the tested samples: Bionate 55D (TR = 0.051) and PET-DLA 65% (average TR = 0.711). The ceramics showed the worst thrombogenic properties (TR = 1.846). All the tested materials were much less thrombogenic than the positive control (TR = 5.639).
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spelling pubmed-98212582023-01-07 Semi-Quantitative Method of Assessing the Thrombogenicity of Biomaterials Intended for Long-Term Blood Contact Gawlikowski, Maciej Major, Roman Mika, Barbara Komorowski, Dariusz Janiczak, Karolina Tkacz, Ewaryst Tamulewicz, Anna Piaseczna, Natalia Materials (Basel) Article Biomaterials used in cardiosurgical implants and artificial valves that have long-term contact with blood pose a great challenge for researchers due to the induction of thrombogenicity. So far, the assessment of the thrombogenicity of biomaterials has been performed with the use of highly subjective descriptive methods, which has made it impossible to compare the results of various experiments. The aim of this paper was to present a new semi-quantitative method of thrombogenicity assessment based on scanning electron microscope (SEM) images of an adhered biological material deposited on the surfaces of prepared samples. The following biomaterials were used to develop the proposed method: Bionate 55D polyurethane, polyether-ether ketone, Ti6Al7Nb alloy, sintered yttria-stabilized zirconium oxide (ZrO(2) + Y(2)O(3)), collagen-coated glass, and bacterial cellulose. The samples were prepared by incubating the biomaterials with platelet-rich plasma. In order to quantify the thrombogenic properties of the biomaterials, a TR parameter based on the fractal dimension was applied. The obtained results confirmed that the use of the fractal dimension enables the quantitative assessment of thrombogenicity and the proper qualification of samples in line with an expert’s judgment. The polyurethanes showed the best thrombogenic properties of the tested samples: Bionate 55D (TR = 0.051) and PET-DLA 65% (average TR = 0.711). The ceramics showed the worst thrombogenic properties (TR = 1.846). All the tested materials were much less thrombogenic than the positive control (TR = 5.639). MDPI 2022-12-21 /pmc/articles/PMC9821258/ /pubmed/36614378 http://dx.doi.org/10.3390/ma16010038 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gawlikowski, Maciej
Major, Roman
Mika, Barbara
Komorowski, Dariusz
Janiczak, Karolina
Tkacz, Ewaryst
Tamulewicz, Anna
Piaseczna, Natalia
Semi-Quantitative Method of Assessing the Thrombogenicity of Biomaterials Intended for Long-Term Blood Contact
title Semi-Quantitative Method of Assessing the Thrombogenicity of Biomaterials Intended for Long-Term Blood Contact
title_full Semi-Quantitative Method of Assessing the Thrombogenicity of Biomaterials Intended for Long-Term Blood Contact
title_fullStr Semi-Quantitative Method of Assessing the Thrombogenicity of Biomaterials Intended for Long-Term Blood Contact
title_full_unstemmed Semi-Quantitative Method of Assessing the Thrombogenicity of Biomaterials Intended for Long-Term Blood Contact
title_short Semi-Quantitative Method of Assessing the Thrombogenicity of Biomaterials Intended for Long-Term Blood Contact
title_sort semi-quantitative method of assessing the thrombogenicity of biomaterials intended for long-term blood contact
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821258/
https://www.ncbi.nlm.nih.gov/pubmed/36614378
http://dx.doi.org/10.3390/ma16010038
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