Cargando…
Krüppel-like Factor 6 Suppresses the Progression of Pancreatic Cancer by Upregulating Activating Transcription Factor 3
Background: As a member of the Krüppel-like factor (KLFs) family, Krüppel-like factor 6 (KLF6) plays a critical role in regulating key cellular functions. Presently, scholars have proved the important role of KLF6 in the tumorigenesis of certain cancers through a large number of experiments. However...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821328/ https://www.ncbi.nlm.nih.gov/pubmed/36615000 http://dx.doi.org/10.3390/jcm12010200 |
_version_ | 1784865670356271104 |
---|---|
author | Xiong, Qunli Zhang, Zhiwei Yang, Yang Xu, Yongfeng Zhou, Ying Zhang, Su Liu, Jinlu Zheng, Ying Zhu, Qing |
author_facet | Xiong, Qunli Zhang, Zhiwei Yang, Yang Xu, Yongfeng Zhou, Ying Zhang, Su Liu, Jinlu Zheng, Ying Zhu, Qing |
author_sort | Xiong, Qunli |
collection | PubMed |
description | Background: As a member of the Krüppel-like factor (KLFs) family, Krüppel-like factor 6 (KLF6) plays a critical role in regulating key cellular functions. Presently, scholars have proved the important role of KLF6 in the tumorigenesis of certain cancers through a large number of experiments. However, gaps still remain in our knowledge of the role of KLF6 in pancreatic cancer (PAAD). Therefore, this paper mainly investigates the role of KLF6 in the progression of pancreatic cancer. Methods: The expression pattern of KLF6 in pancreatic cancer was explored in pancreatic cancer tissues and cell lines. Then, we investigated the prognostic value of KLF6 in pancreatic cancer by immunohistochemical assays. Next, Cell Counting Kit-8 (CCK8) and clone information assays were employed to explore the proliferation of PAAD affected by KLF6. The metastasis and epithelial-mesenchymal transition (EMT) abilities affected by KLF6 were identified through transwell invasion as well as migration assays and western blots. Finally, the TRRUST tool was used to analyze the potential targeted genes of KLF6. The results were verified by Quantificational Real-time Polymerase Chain Reaction (qRT-PCR), western blot and rescue assays. Results: KLF6 expresses lowly in pancreatic cancer compared to corresponding normal tissues and relates to poor survival times. Overexpression of KLF6 inhibits the proliferation, metastasis, and EMT progression in pancreatic cancer cells. Further studies suggest that KLF6 could upregulate ATF3 in PAAD. Conclusions: Our results suggest that KLF6 can be a useful factor in predicting the prognosis of PAAD patients and that it inhibits the progression of pancreatic cancer by upregulating activating transcription factor 3 (ATF3). |
format | Online Article Text |
id | pubmed-9821328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98213282023-01-07 Krüppel-like Factor 6 Suppresses the Progression of Pancreatic Cancer by Upregulating Activating Transcription Factor 3 Xiong, Qunli Zhang, Zhiwei Yang, Yang Xu, Yongfeng Zhou, Ying Zhang, Su Liu, Jinlu Zheng, Ying Zhu, Qing J Clin Med Article Background: As a member of the Krüppel-like factor (KLFs) family, Krüppel-like factor 6 (KLF6) plays a critical role in regulating key cellular functions. Presently, scholars have proved the important role of KLF6 in the tumorigenesis of certain cancers through a large number of experiments. However, gaps still remain in our knowledge of the role of KLF6 in pancreatic cancer (PAAD). Therefore, this paper mainly investigates the role of KLF6 in the progression of pancreatic cancer. Methods: The expression pattern of KLF6 in pancreatic cancer was explored in pancreatic cancer tissues and cell lines. Then, we investigated the prognostic value of KLF6 in pancreatic cancer by immunohistochemical assays. Next, Cell Counting Kit-8 (CCK8) and clone information assays were employed to explore the proliferation of PAAD affected by KLF6. The metastasis and epithelial-mesenchymal transition (EMT) abilities affected by KLF6 were identified through transwell invasion as well as migration assays and western blots. Finally, the TRRUST tool was used to analyze the potential targeted genes of KLF6. The results were verified by Quantificational Real-time Polymerase Chain Reaction (qRT-PCR), western blot and rescue assays. Results: KLF6 expresses lowly in pancreatic cancer compared to corresponding normal tissues and relates to poor survival times. Overexpression of KLF6 inhibits the proliferation, metastasis, and EMT progression in pancreatic cancer cells. Further studies suggest that KLF6 could upregulate ATF3 in PAAD. Conclusions: Our results suggest that KLF6 can be a useful factor in predicting the prognosis of PAAD patients and that it inhibits the progression of pancreatic cancer by upregulating activating transcription factor 3 (ATF3). MDPI 2022-12-27 /pmc/articles/PMC9821328/ /pubmed/36615000 http://dx.doi.org/10.3390/jcm12010200 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xiong, Qunli Zhang, Zhiwei Yang, Yang Xu, Yongfeng Zhou, Ying Zhang, Su Liu, Jinlu Zheng, Ying Zhu, Qing Krüppel-like Factor 6 Suppresses the Progression of Pancreatic Cancer by Upregulating Activating Transcription Factor 3 |
title | Krüppel-like Factor 6 Suppresses the Progression of Pancreatic Cancer by Upregulating Activating Transcription Factor 3 |
title_full | Krüppel-like Factor 6 Suppresses the Progression of Pancreatic Cancer by Upregulating Activating Transcription Factor 3 |
title_fullStr | Krüppel-like Factor 6 Suppresses the Progression of Pancreatic Cancer by Upregulating Activating Transcription Factor 3 |
title_full_unstemmed | Krüppel-like Factor 6 Suppresses the Progression of Pancreatic Cancer by Upregulating Activating Transcription Factor 3 |
title_short | Krüppel-like Factor 6 Suppresses the Progression of Pancreatic Cancer by Upregulating Activating Transcription Factor 3 |
title_sort | krüppel-like factor 6 suppresses the progression of pancreatic cancer by upregulating activating transcription factor 3 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821328/ https://www.ncbi.nlm.nih.gov/pubmed/36615000 http://dx.doi.org/10.3390/jcm12010200 |
work_keys_str_mv | AT xiongqunli kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 AT zhangzhiwei kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 AT yangyang kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 AT xuyongfeng kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 AT zhouying kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 AT zhangsu kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 AT liujinlu kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 AT zhengying kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 AT zhuqing kruppellikefactor6suppressestheprogressionofpancreaticcancerbyupregulatingactivatingtranscriptionfactor3 |