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Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model
Ocular herpes simplex type 1 (HSV-1) infections can trigger conjunctivitis, keratitis, uveitis, and occasionally retinitis, and is a major cause of blindness worldwide. The infections are lifelong and can often recrudesce during periods of stress or immune suppression. Currently HSV-1 infections of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821494/ https://www.ncbi.nlm.nih.gov/pubmed/36607992 http://dx.doi.org/10.1371/journal.pone.0279462 |
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author | Lappin, Michael Wotman, Kathryn Chow, Lyndah Williams, Maggie Hawley, Jennifer Dow, Steven |
author_facet | Lappin, Michael Wotman, Kathryn Chow, Lyndah Williams, Maggie Hawley, Jennifer Dow, Steven |
author_sort | Lappin, Michael |
collection | PubMed |
description | Ocular herpes simplex type 1 (HSV-1) infections can trigger conjunctivitis, keratitis, uveitis, and occasionally retinitis, and is a major cause of blindness worldwide. The infections are lifelong and can often recrudesce during periods of stress or immune suppression. Currently HSV-1 infections of the eye are managed primarily with anti-viral eye drops, which require frequent administration, can cause irritation, and may take weeks for full resolution of symptoms. We therefore evaluated the effectiveness of an ocular immune activating nanoparticle eye drop as a novel approach to treating HSV-1 infection, using a cat feline herpesvirus -1 (FHV-1) ocular infection model. In vitro studies demonstrated significant induction of both type I and II interferon responses by the liposome-dual TLR 3/9 agonist nanoparticles, along with suppression of FHV-1 replication. In cats with naturally occurring eye infections either proven or suspected to involve FHV-1, ocular nanoparticle treated animals experienced resolution of signs within several days of treatment, including resolution of keratitis and corneal ulcers. In a cat model of recrudescent FHV-1 infection, cats treated twice daily with immune nanoparticle eye drops experienced significant lessening of ocular signs of infection and significantly fewer episodes of viral shedding compared to control cats. Treatment was well-tolerated by all cats, without signs of drug-induced ocular irritation. We concluded therefore that non-specific ocular immunotherapy offers significant promise as a novel approach to treatment of HSV-1 and FHV-1 ocular infections. |
format | Online Article Text |
id | pubmed-9821494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-98214942023-01-07 Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model Lappin, Michael Wotman, Kathryn Chow, Lyndah Williams, Maggie Hawley, Jennifer Dow, Steven PLoS One Research Article Ocular herpes simplex type 1 (HSV-1) infections can trigger conjunctivitis, keratitis, uveitis, and occasionally retinitis, and is a major cause of blindness worldwide. The infections are lifelong and can often recrudesce during periods of stress or immune suppression. Currently HSV-1 infections of the eye are managed primarily with anti-viral eye drops, which require frequent administration, can cause irritation, and may take weeks for full resolution of symptoms. We therefore evaluated the effectiveness of an ocular immune activating nanoparticle eye drop as a novel approach to treating HSV-1 infection, using a cat feline herpesvirus -1 (FHV-1) ocular infection model. In vitro studies demonstrated significant induction of both type I and II interferon responses by the liposome-dual TLR 3/9 agonist nanoparticles, along with suppression of FHV-1 replication. In cats with naturally occurring eye infections either proven or suspected to involve FHV-1, ocular nanoparticle treated animals experienced resolution of signs within several days of treatment, including resolution of keratitis and corneal ulcers. In a cat model of recrudescent FHV-1 infection, cats treated twice daily with immune nanoparticle eye drops experienced significant lessening of ocular signs of infection and significantly fewer episodes of viral shedding compared to control cats. Treatment was well-tolerated by all cats, without signs of drug-induced ocular irritation. We concluded therefore that non-specific ocular immunotherapy offers significant promise as a novel approach to treatment of HSV-1 and FHV-1 ocular infections. Public Library of Science 2023-01-06 /pmc/articles/PMC9821494/ /pubmed/36607992 http://dx.doi.org/10.1371/journal.pone.0279462 Text en © 2023 Lappin et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Lappin, Michael Wotman, Kathryn Chow, Lyndah Williams, Maggie Hawley, Jennifer Dow, Steven Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model |
title | Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model |
title_full | Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model |
title_fullStr | Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model |
title_full_unstemmed | Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model |
title_short | Nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model |
title_sort | nanoparticle ocular immunotherapy for herpesvirus surface eye infections evaluated in cat infection model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821494/ https://www.ncbi.nlm.nih.gov/pubmed/36607992 http://dx.doi.org/10.1371/journal.pone.0279462 |
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