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Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway

Cervical carcinoma (CC) is the second most prevalent gynecologic cancer in females across the world. To obtain a better understanding of the mechanisms underlying the development of CC, high-resolution label-free mass spectrometry was performed on CC and adjacent normal tissues from eight patients....

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Autores principales: Hu, Wei, Hu, Yaorui, Pei, Yao, Li, Rongrong, Xu, Fuyi, Chi, Xiaodong, Mi, Jia, Bergquist, Jonas, Lu, Lu, Zhang, Luping, Yang, Chunhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821498/
https://www.ncbi.nlm.nih.gov/pubmed/36614304
http://dx.doi.org/10.3390/ijms24010861
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author Hu, Wei
Hu, Yaorui
Pei, Yao
Li, Rongrong
Xu, Fuyi
Chi, Xiaodong
Mi, Jia
Bergquist, Jonas
Lu, Lu
Zhang, Luping
Yang, Chunhua
author_facet Hu, Wei
Hu, Yaorui
Pei, Yao
Li, Rongrong
Xu, Fuyi
Chi, Xiaodong
Mi, Jia
Bergquist, Jonas
Lu, Lu
Zhang, Luping
Yang, Chunhua
author_sort Hu, Wei
collection PubMed
description Cervical carcinoma (CC) is the second most prevalent gynecologic cancer in females across the world. To obtain a better understanding of the mechanisms underlying the development of CC, high-resolution label-free mass spectrometry was performed on CC and adjacent normal tissues from eight patients. A total of 2631 proteins were identified, and 46 significant differently expressed proteins (DEPs) were found between CC and normal tissues (p < 0.01, fold change >10 or <0.1). Ingenuity pathway analysis revealed that the majority of the proteins were involved in the regulation of eIF4 and p70S6K signaling and mTOR signaling. Among 46 DEPs, Integrinβ6 (ITGB6), PPP1CB, TMPO, PTGES3 (P23) and DTX3L were significantly upregulated, while Desmin (DES) was significantly downregulated in CC tissues compared with the adjacent normal tissues. In in vivo and in vitro experiments, DTX3L knockdown suppressed CC cell proliferation, migration, invasion and xenograft tumorigenesis, and enhanced cell apoptosis. Combination of silencing DTX3L and cisplatin treatment induced higher apoptosis percentage compared to cisplatin treatment alone. Moreover, DTX3L silencing inhibited the PI3K/AKT/mTOR signal pathway. Thus, our results suggested DTX3L could regulate CC progression through the PI3K/AKT/mTOR signal pathway and is potentially a novel biomarker and therapeutic target for CC.
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spelling pubmed-98214982023-01-07 Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway Hu, Wei Hu, Yaorui Pei, Yao Li, Rongrong Xu, Fuyi Chi, Xiaodong Mi, Jia Bergquist, Jonas Lu, Lu Zhang, Luping Yang, Chunhua Int J Mol Sci Article Cervical carcinoma (CC) is the second most prevalent gynecologic cancer in females across the world. To obtain a better understanding of the mechanisms underlying the development of CC, high-resolution label-free mass spectrometry was performed on CC and adjacent normal tissues from eight patients. A total of 2631 proteins were identified, and 46 significant differently expressed proteins (DEPs) were found between CC and normal tissues (p < 0.01, fold change >10 or <0.1). Ingenuity pathway analysis revealed that the majority of the proteins were involved in the regulation of eIF4 and p70S6K signaling and mTOR signaling. Among 46 DEPs, Integrinβ6 (ITGB6), PPP1CB, TMPO, PTGES3 (P23) and DTX3L were significantly upregulated, while Desmin (DES) was significantly downregulated in CC tissues compared with the adjacent normal tissues. In in vivo and in vitro experiments, DTX3L knockdown suppressed CC cell proliferation, migration, invasion and xenograft tumorigenesis, and enhanced cell apoptosis. Combination of silencing DTX3L and cisplatin treatment induced higher apoptosis percentage compared to cisplatin treatment alone. Moreover, DTX3L silencing inhibited the PI3K/AKT/mTOR signal pathway. Thus, our results suggested DTX3L could regulate CC progression through the PI3K/AKT/mTOR signal pathway and is potentially a novel biomarker and therapeutic target for CC. MDPI 2023-01-03 /pmc/articles/PMC9821498/ /pubmed/36614304 http://dx.doi.org/10.3390/ijms24010861 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Wei
Hu, Yaorui
Pei, Yao
Li, Rongrong
Xu, Fuyi
Chi, Xiaodong
Mi, Jia
Bergquist, Jonas
Lu, Lu
Zhang, Luping
Yang, Chunhua
Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway
title Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway
title_full Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway
title_fullStr Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway
title_full_unstemmed Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway
title_short Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway
title_sort silencing dtx3l inhibits the progression of cervical carcinoma by regulating pi3k/akt/mtor signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821498/
https://www.ncbi.nlm.nih.gov/pubmed/36614304
http://dx.doi.org/10.3390/ijms24010861
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