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Enhanced Anti-Inflammatory Effect of the Combination of Lactiplantibacillus plantarum LS/07 with Methotrexate Compared to Their Monotherapies Studied in Experimental Arthritis

The gut microbiome (GM) of rheumatic arthritis (RA) patients is often altered in composition and function. Moreover, methotrexate (MTX), one of the most frequently used disease-modifying antirheumatic drugs, is known to negatively affect GM composition. The modulation of immune system activity is on...

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Autores principales: Pružinská, Katarína, Slovák, Lukáš, Dráfi, František, Poništ, Silvester, Juránek, Ivo, Chrastina, Martin, Švík, Karol, Strojný, Ladislav, Ambro, Ľuboš, Bauerová, Katarína
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822002/
https://www.ncbi.nlm.nih.gov/pubmed/36615489
http://dx.doi.org/10.3390/molecules28010297
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author Pružinská, Katarína
Slovák, Lukáš
Dráfi, František
Poništ, Silvester
Juránek, Ivo
Chrastina, Martin
Švík, Karol
Strojný, Ladislav
Ambro, Ľuboš
Bauerová, Katarína
author_facet Pružinská, Katarína
Slovák, Lukáš
Dráfi, František
Poništ, Silvester
Juránek, Ivo
Chrastina, Martin
Švík, Karol
Strojný, Ladislav
Ambro, Ľuboš
Bauerová, Katarína
author_sort Pružinská, Katarína
collection PubMed
description The gut microbiome (GM) of rheumatic arthritis (RA) patients is often altered in composition and function. Moreover, methotrexate (MTX), one of the most frequently used disease-modifying antirheumatic drugs, is known to negatively affect GM composition. The modulation of immune system activity is one of the therapeutic benefits of probiotics. The aim of the current investigation was to determine the impact of MTX therapy combined with one of the Lactobacillus strains, Lactoplantibacillus plantarum LS/07 (LB), on adjuvant arthritis (AA) in rats. Methods focused on biometric and inflammatory parameters in AA, particularly on plasmatic levels of IL-17A, MMP-9, and MCP-1, and the activities of gamma-glutamyl transferase in the spleen and joints were applied. Enhancing the effect of MTX, LB positively influenced all biometric and inflammatory parameters. The findings of the present study may be of help in proposing novel therapeutic strategies for RA patients.
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spelling pubmed-98220022023-01-07 Enhanced Anti-Inflammatory Effect of the Combination of Lactiplantibacillus plantarum LS/07 with Methotrexate Compared to Their Monotherapies Studied in Experimental Arthritis Pružinská, Katarína Slovák, Lukáš Dráfi, František Poništ, Silvester Juránek, Ivo Chrastina, Martin Švík, Karol Strojný, Ladislav Ambro, Ľuboš Bauerová, Katarína Molecules Article The gut microbiome (GM) of rheumatic arthritis (RA) patients is often altered in composition and function. Moreover, methotrexate (MTX), one of the most frequently used disease-modifying antirheumatic drugs, is known to negatively affect GM composition. The modulation of immune system activity is one of the therapeutic benefits of probiotics. The aim of the current investigation was to determine the impact of MTX therapy combined with one of the Lactobacillus strains, Lactoplantibacillus plantarum LS/07 (LB), on adjuvant arthritis (AA) in rats. Methods focused on biometric and inflammatory parameters in AA, particularly on plasmatic levels of IL-17A, MMP-9, and MCP-1, and the activities of gamma-glutamyl transferase in the spleen and joints were applied. Enhancing the effect of MTX, LB positively influenced all biometric and inflammatory parameters. The findings of the present study may be of help in proposing novel therapeutic strategies for RA patients. MDPI 2022-12-30 /pmc/articles/PMC9822002/ /pubmed/36615489 http://dx.doi.org/10.3390/molecules28010297 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pružinská, Katarína
Slovák, Lukáš
Dráfi, František
Poništ, Silvester
Juránek, Ivo
Chrastina, Martin
Švík, Karol
Strojný, Ladislav
Ambro, Ľuboš
Bauerová, Katarína
Enhanced Anti-Inflammatory Effect of the Combination of Lactiplantibacillus plantarum LS/07 with Methotrexate Compared to Their Monotherapies Studied in Experimental Arthritis
title Enhanced Anti-Inflammatory Effect of the Combination of Lactiplantibacillus plantarum LS/07 with Methotrexate Compared to Their Monotherapies Studied in Experimental Arthritis
title_full Enhanced Anti-Inflammatory Effect of the Combination of Lactiplantibacillus plantarum LS/07 with Methotrexate Compared to Their Monotherapies Studied in Experimental Arthritis
title_fullStr Enhanced Anti-Inflammatory Effect of the Combination of Lactiplantibacillus plantarum LS/07 with Methotrexate Compared to Their Monotherapies Studied in Experimental Arthritis
title_full_unstemmed Enhanced Anti-Inflammatory Effect of the Combination of Lactiplantibacillus plantarum LS/07 with Methotrexate Compared to Their Monotherapies Studied in Experimental Arthritis
title_short Enhanced Anti-Inflammatory Effect of the Combination of Lactiplantibacillus plantarum LS/07 with Methotrexate Compared to Their Monotherapies Studied in Experimental Arthritis
title_sort enhanced anti-inflammatory effect of the combination of lactiplantibacillus plantarum ls/07 with methotrexate compared to their monotherapies studied in experimental arthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822002/
https://www.ncbi.nlm.nih.gov/pubmed/36615489
http://dx.doi.org/10.3390/molecules28010297
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