A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides

A strain of marine actinomycetes was isolated from an intertidal zone and identified as Streptomyces cinereoruber. Through the fermentation of this strain, a compound with fungicidal activity was extracted and purified. Using mass spectrometry (MS) and nuclear magnetic resonance (NMR) data, the meta...

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Autores principales: Liu, Bin, Li, Xiaomeng, Wang, Weiguo, Wang, Xin, Aihaiti, Pahaiding, Lin, Tingtang, Fu, Zishuo, Xu, Rui, Wu, Mengqi, Li, Zhong, Zhang, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822012/
https://www.ncbi.nlm.nih.gov/pubmed/36615212
http://dx.doi.org/10.3390/molecules28010017
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author Liu, Bin
Li, Xiaomeng
Wang, Weiguo
Wang, Xin
Aihaiti, Pahaiding
Lin, Tingtang
Fu, Zishuo
Xu, Rui
Wu, Mengqi
Li, Zhong
Zhang, Yang
author_facet Liu, Bin
Li, Xiaomeng
Wang, Weiguo
Wang, Xin
Aihaiti, Pahaiding
Lin, Tingtang
Fu, Zishuo
Xu, Rui
Wu, Mengqi
Li, Zhong
Zhang, Yang
author_sort Liu, Bin
collection PubMed
description A strain of marine actinomycetes was isolated from an intertidal zone and identified as Streptomyces cinereoruber. Through the fermentation of this strain, a compound with fungicidal activity was extracted and purified. Using mass spectrometry (MS) and nuclear magnetic resonance (NMR) data, the metabolite was determined to be an aurone. The toxicity of the aurone toward four kinds of tumor cells—SH-SY5Y, HepG2, A549, and HeLa cells—was verified by the MTT method, delivering IC(50) values of 41.81, 47.19, 63.95, and 51.92 μg/mL, respectively. Greenhouse bioassay showed that the aurone exhibited a high fungicidal activity against powder mildew (Botrytis cinerea), cucurbits powder mildew (Sphaerotheca fuliginea (Schlecht ex Ff.) Poll), and rice blast (Pyricularia oryzae).
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spelling pubmed-98220122023-01-07 A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides Liu, Bin Li, Xiaomeng Wang, Weiguo Wang, Xin Aihaiti, Pahaiding Lin, Tingtang Fu, Zishuo Xu, Rui Wu, Mengqi Li, Zhong Zhang, Yang Molecules Article A strain of marine actinomycetes was isolated from an intertidal zone and identified as Streptomyces cinereoruber. Through the fermentation of this strain, a compound with fungicidal activity was extracted and purified. Using mass spectrometry (MS) and nuclear magnetic resonance (NMR) data, the metabolite was determined to be an aurone. The toxicity of the aurone toward four kinds of tumor cells—SH-SY5Y, HepG2, A549, and HeLa cells—was verified by the MTT method, delivering IC(50) values of 41.81, 47.19, 63.95, and 51.92 μg/mL, respectively. Greenhouse bioassay showed that the aurone exhibited a high fungicidal activity against powder mildew (Botrytis cinerea), cucurbits powder mildew (Sphaerotheca fuliginea (Schlecht ex Ff.) Poll), and rice blast (Pyricularia oryzae). MDPI 2022-12-20 /pmc/articles/PMC9822012/ /pubmed/36615212 http://dx.doi.org/10.3390/molecules28010017 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Bin
Li, Xiaomeng
Wang, Weiguo
Wang, Xin
Aihaiti, Pahaiding
Lin, Tingtang
Fu, Zishuo
Xu, Rui
Wu, Mengqi
Li, Zhong
Zhang, Yang
A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides
title A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides
title_full A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides
title_fullStr A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides
title_full_unstemmed A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides
title_short A New Method of Preparing Aurone by Marine Actinomycetes and Its Potential Application in Agricultural Fungicides
title_sort new method of preparing aurone by marine actinomycetes and its potential application in agricultural fungicides
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822012/
https://www.ncbi.nlm.nih.gov/pubmed/36615212
http://dx.doi.org/10.3390/molecules28010017
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