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Locking the GFP Fluorophore to Enhance Its Emission Intensity

The Green Fluorescent Protein (GFP) and its analogues have been widely used as fluorescent biomarkers in cell biology. Yet, the chromophore responsible for the fluorescence of the GFP is not emissive when isolated in solution, outside the protein environment. The most accepted explanation is that th...

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Detalles Bibliográficos
Autores principales: Ferreira, Joana R. M., Esteves, Cátia I. C., Marques, Maria Manuel B., Guieu, Samuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822164/
https://www.ncbi.nlm.nih.gov/pubmed/36615428
http://dx.doi.org/10.3390/molecules28010234