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Extracts from European Propolises as Potent Tyrosinase Inhibitors

Tyrosinase is a key enzyme in the melanogenesis pathway. Melanin, the product of this process, is the main pigment of the human skin and a major protection factor against harmful ultraviolet radiation (UVR). Increased melanin synthesis due to tyrosinase hyperactivity can cause hyperpigmentation diso...

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Autores principales: Widelski, Jarosław, Gaweł-Bęben, Katarzyna, Czech, Karolina, Paluch, Emil, Bortkiewicz, Olga, Kozachok, Solomiia, Mroczek, Tomasz, Okińczyc, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822166/
https://www.ncbi.nlm.nih.gov/pubmed/36615251
http://dx.doi.org/10.3390/molecules28010055
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author Widelski, Jarosław
Gaweł-Bęben, Katarzyna
Czech, Karolina
Paluch, Emil
Bortkiewicz, Olga
Kozachok, Solomiia
Mroczek, Tomasz
Okińczyc, Piotr
author_facet Widelski, Jarosław
Gaweł-Bęben, Katarzyna
Czech, Karolina
Paluch, Emil
Bortkiewicz, Olga
Kozachok, Solomiia
Mroczek, Tomasz
Okińczyc, Piotr
author_sort Widelski, Jarosław
collection PubMed
description Tyrosinase is a key enzyme in the melanogenesis pathway. Melanin, the product of this process, is the main pigment of the human skin and a major protection factor against harmful ultraviolet radiation (UVR). Increased melanin synthesis due to tyrosinase hyperactivity can cause hyperpigmentation disorders, which in consequence causes freckles, age spots, melasma, or postinflammatory hyperpigmentation. Tyrosinase overproduction and hyperactivity are triggered by the ageing processes and skin inflammation as a result of oxidative stress. Therefore, the control of tyrosinase activity is the main goal of the prevention and treatment of pigmentation disorders. Natural products, especially propolis, according to their phytochemical profile abundant in polyphenols, is a very rich resource of new potential tyrosinase inhibitors. Therefore, this study focused on the assessment of the tyrosinase inhibitory potential of six extracts obtained from the European propolis samples of various origins. The results showed the potent inhibitory activity of all tested propolis extracts towards commercially available mushroom tyrosinase. The four most active propolis extracts showed inhibitory activity in the range of 86.66–93.25%. Apart from the evaluation of the tyrosinase inhibition, the performed research included UHPLC–DAD–MS/MS (ultra high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry) phytochemical profiling as well as antioxidant activity assessment using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the 2,2”-azino-bis(3-ethylbenzothiazoline-6-sulfuric acid (ABTS) radical scavenging tests. Moreover, statistical analysis was used to correlate the tyrosinase inhibitory and antioxidant activities of propolis extracts with their phytochemical composition. To summarise, the results of our research showed that tested propolis extracts could be used for skin cosmeceutical and medical applications.
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spelling pubmed-98221662023-01-07 Extracts from European Propolises as Potent Tyrosinase Inhibitors Widelski, Jarosław Gaweł-Bęben, Katarzyna Czech, Karolina Paluch, Emil Bortkiewicz, Olga Kozachok, Solomiia Mroczek, Tomasz Okińczyc, Piotr Molecules Article Tyrosinase is a key enzyme in the melanogenesis pathway. Melanin, the product of this process, is the main pigment of the human skin and a major protection factor against harmful ultraviolet radiation (UVR). Increased melanin synthesis due to tyrosinase hyperactivity can cause hyperpigmentation disorders, which in consequence causes freckles, age spots, melasma, or postinflammatory hyperpigmentation. Tyrosinase overproduction and hyperactivity are triggered by the ageing processes and skin inflammation as a result of oxidative stress. Therefore, the control of tyrosinase activity is the main goal of the prevention and treatment of pigmentation disorders. Natural products, especially propolis, according to their phytochemical profile abundant in polyphenols, is a very rich resource of new potential tyrosinase inhibitors. Therefore, this study focused on the assessment of the tyrosinase inhibitory potential of six extracts obtained from the European propolis samples of various origins. The results showed the potent inhibitory activity of all tested propolis extracts towards commercially available mushroom tyrosinase. The four most active propolis extracts showed inhibitory activity in the range of 86.66–93.25%. Apart from the evaluation of the tyrosinase inhibition, the performed research included UHPLC–DAD–MS/MS (ultra high performance liquid chromatography coupled with diode array detection and tandem mass spectrometry) phytochemical profiling as well as antioxidant activity assessment using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and the 2,2”-azino-bis(3-ethylbenzothiazoline-6-sulfuric acid (ABTS) radical scavenging tests. Moreover, statistical analysis was used to correlate the tyrosinase inhibitory and antioxidant activities of propolis extracts with their phytochemical composition. To summarise, the results of our research showed that tested propolis extracts could be used for skin cosmeceutical and medical applications. MDPI 2022-12-21 /pmc/articles/PMC9822166/ /pubmed/36615251 http://dx.doi.org/10.3390/molecules28010055 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Widelski, Jarosław
Gaweł-Bęben, Katarzyna
Czech, Karolina
Paluch, Emil
Bortkiewicz, Olga
Kozachok, Solomiia
Mroczek, Tomasz
Okińczyc, Piotr
Extracts from European Propolises as Potent Tyrosinase Inhibitors
title Extracts from European Propolises as Potent Tyrosinase Inhibitors
title_full Extracts from European Propolises as Potent Tyrosinase Inhibitors
title_fullStr Extracts from European Propolises as Potent Tyrosinase Inhibitors
title_full_unstemmed Extracts from European Propolises as Potent Tyrosinase Inhibitors
title_short Extracts from European Propolises as Potent Tyrosinase Inhibitors
title_sort extracts from european propolises as potent tyrosinase inhibitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822166/
https://www.ncbi.nlm.nih.gov/pubmed/36615251
http://dx.doi.org/10.3390/molecules28010055
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