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Green Synthesized Zinc Oxide Nanoparticles Based on Cestrum diurnum L. of Potential Antiviral Activity against Human Corona 229-E Virus

SARS-CoV-2 has caused more than 596 million infections and 6 million fatalities globally. Looking for urgent medication for prevention, treatment, and rehabilitation is obligatory. Plant extracts and green synthesized nanoparticles have numerous biological activities, including antiviral activity. H...

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Autores principales: Alrabayah, Ibrahim N., Elhawary, Seham S., Kandil, Zeinab A., El-Kadder, Essam M. Abd, Moemen, Yasmine S., Saleh, Abdulrahman M., El Raey, Mohamed A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822259/
https://www.ncbi.nlm.nih.gov/pubmed/36615461
http://dx.doi.org/10.3390/molecules28010266
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author Alrabayah, Ibrahim N.
Elhawary, Seham S.
Kandil, Zeinab A.
El-Kadder, Essam M. Abd
Moemen, Yasmine S.
Saleh, Abdulrahman M.
El Raey, Mohamed A.
author_facet Alrabayah, Ibrahim N.
Elhawary, Seham S.
Kandil, Zeinab A.
El-Kadder, Essam M. Abd
Moemen, Yasmine S.
Saleh, Abdulrahman M.
El Raey, Mohamed A.
author_sort Alrabayah, Ibrahim N.
collection PubMed
description SARS-CoV-2 has caused more than 596 million infections and 6 million fatalities globally. Looking for urgent medication for prevention, treatment, and rehabilitation is obligatory. Plant extracts and green synthesized nanoparticles have numerous biological activities, including antiviral activity. HPLC analysis of C. dirnum L. leaf extract showed that catechin, ferulic acid, chlorogenic acid, and syringic acid were the most major compounds, with concentrations of 1425.16, 1004.68, 207.46, and 158.95 µg/g, respectively. Zinc nanoparticles were biosynthesized using zinc acetate and C. dirnum extract. TEM analysis revealed that the particle size of ZnO-NPs varied between 3.406 and 4.857 nm. An XRD study showed the existence of hexagonal crystals of ZnO-NPs with an average size of 12.11 nm. Both ZnO-NPs (IC(50) = 7.01 and CC(50) = 145.77) and C. dirnum L. extract (IC(50) = 61.15 and CC(50) = 145.87 µg/mL) showed antiviral activity against HCOV-229E, but their combination (IC(50) = 2.41 and CC(50) = 179.23) showed higher activity than both. Molecular docking was used to investigate the affinity of some metabolites against the HCOV-229E main protease. Chlorogenic acid, solanidine, and catchin showed high affinity (−7.13, −6.95, and −6.52), compared to the ligand MDP (−5.66 Kcal/mol). Cestrum dinurum extract and ZnO-NPs combination should be subjected to further studies to be used as an antiviral drug.
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spelling pubmed-98222592023-01-07 Green Synthesized Zinc Oxide Nanoparticles Based on Cestrum diurnum L. of Potential Antiviral Activity against Human Corona 229-E Virus Alrabayah, Ibrahim N. Elhawary, Seham S. Kandil, Zeinab A. El-Kadder, Essam M. Abd Moemen, Yasmine S. Saleh, Abdulrahman M. El Raey, Mohamed A. Molecules Article SARS-CoV-2 has caused more than 596 million infections and 6 million fatalities globally. Looking for urgent medication for prevention, treatment, and rehabilitation is obligatory. Plant extracts and green synthesized nanoparticles have numerous biological activities, including antiviral activity. HPLC analysis of C. dirnum L. leaf extract showed that catechin, ferulic acid, chlorogenic acid, and syringic acid were the most major compounds, with concentrations of 1425.16, 1004.68, 207.46, and 158.95 µg/g, respectively. Zinc nanoparticles were biosynthesized using zinc acetate and C. dirnum extract. TEM analysis revealed that the particle size of ZnO-NPs varied between 3.406 and 4.857 nm. An XRD study showed the existence of hexagonal crystals of ZnO-NPs with an average size of 12.11 nm. Both ZnO-NPs (IC(50) = 7.01 and CC(50) = 145.77) and C. dirnum L. extract (IC(50) = 61.15 and CC(50) = 145.87 µg/mL) showed antiviral activity against HCOV-229E, but their combination (IC(50) = 2.41 and CC(50) = 179.23) showed higher activity than both. Molecular docking was used to investigate the affinity of some metabolites against the HCOV-229E main protease. Chlorogenic acid, solanidine, and catchin showed high affinity (−7.13, −6.95, and −6.52), compared to the ligand MDP (−5.66 Kcal/mol). Cestrum dinurum extract and ZnO-NPs combination should be subjected to further studies to be used as an antiviral drug. MDPI 2022-12-28 /pmc/articles/PMC9822259/ /pubmed/36615461 http://dx.doi.org/10.3390/molecules28010266 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alrabayah, Ibrahim N.
Elhawary, Seham S.
Kandil, Zeinab A.
El-Kadder, Essam M. Abd
Moemen, Yasmine S.
Saleh, Abdulrahman M.
El Raey, Mohamed A.
Green Synthesized Zinc Oxide Nanoparticles Based on Cestrum diurnum L. of Potential Antiviral Activity against Human Corona 229-E Virus
title Green Synthesized Zinc Oxide Nanoparticles Based on Cestrum diurnum L. of Potential Antiviral Activity against Human Corona 229-E Virus
title_full Green Synthesized Zinc Oxide Nanoparticles Based on Cestrum diurnum L. of Potential Antiviral Activity against Human Corona 229-E Virus
title_fullStr Green Synthesized Zinc Oxide Nanoparticles Based on Cestrum diurnum L. of Potential Antiviral Activity against Human Corona 229-E Virus
title_full_unstemmed Green Synthesized Zinc Oxide Nanoparticles Based on Cestrum diurnum L. of Potential Antiviral Activity against Human Corona 229-E Virus
title_short Green Synthesized Zinc Oxide Nanoparticles Based on Cestrum diurnum L. of Potential Antiviral Activity against Human Corona 229-E Virus
title_sort green synthesized zinc oxide nanoparticles based on cestrum diurnum l. of potential antiviral activity against human corona 229-e virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822259/
https://www.ncbi.nlm.nih.gov/pubmed/36615461
http://dx.doi.org/10.3390/molecules28010266
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