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Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity

Green syntheses of metallic nanoparticles using plant extracts as effective sources of reductants and stabilizers have attracted decent popularity due to their non-toxicity, environmental friendliness and rapid nature. The current study demonstrates the ecofriendly, facile and inexpensive synthesis...

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Autores principales: Ajlouni, Abdul-Wali, Hamdan, Eman H., Alshalawi, Rasha Awwadh Eid, Shaik, Mohammed Rafi, Khan, Mujeeb, Kuniyil, Mufsir, Alwarthan, Abdulrahman, Ansari, Mohammad Azam, Khan, Merajuddin, Alkhathlan, Hamad Z., Shaik, Jilani P., Adil, Syed Farooq
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822267/
https://www.ncbi.nlm.nih.gov/pubmed/36615440
http://dx.doi.org/10.3390/molecules28010246
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author Ajlouni, Abdul-Wali
Hamdan, Eman H.
Alshalawi, Rasha Awwadh Eid
Shaik, Mohammed Rafi
Khan, Mujeeb
Kuniyil, Mufsir
Alwarthan, Abdulrahman
Ansari, Mohammad Azam
Khan, Merajuddin
Alkhathlan, Hamad Z.
Shaik, Jilani P.
Adil, Syed Farooq
author_facet Ajlouni, Abdul-Wali
Hamdan, Eman H.
Alshalawi, Rasha Awwadh Eid
Shaik, Mohammed Rafi
Khan, Mujeeb
Kuniyil, Mufsir
Alwarthan, Abdulrahman
Ansari, Mohammad Azam
Khan, Merajuddin
Alkhathlan, Hamad Z.
Shaik, Jilani P.
Adil, Syed Farooq
author_sort Ajlouni, Abdul-Wali
collection PubMed
description Green syntheses of metallic nanoparticles using plant extracts as effective sources of reductants and stabilizers have attracted decent popularity due to their non-toxicity, environmental friendliness and rapid nature. The current study demonstrates the ecofriendly, facile and inexpensive synthesis of silver nanoparticles (AP-AgNPs) using the extract of aerial parts of the Anthemis pseudocotula Boiss. plant (AP). Herein, the aerial parts extract of AP performed a twin role of a reducing as well as a stabilizing agent. The green synthesized AP-AgNPs were characterized by several techniques such as XRD, UV-Vis, FT-IR, TEM, SEM and EDX. Furthermore, the antimicrobial and antibiofilm activity of as-prepared AP-AgNPs were examined by a standard two-fold microbroth dilution method and tissue culture plate methods, respectively, against several Gram-negative and Gram-positive bacterial strains and fungal species such as Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), multidrug-resistant Pseudomonas aeruginosa (MDR-PA) and Acinetobacter baumannii (MDR-AB), methicillin-resistant S. aureus (MRSA) and Candida albicans (C. albicans) strains. The antimicrobial activity results clearly indicated that the Gram-negative bacteria MDR-PA was most affected by AgNPs as compared to other Gram-negative and Gram-positive bacteria and fungi C. albicans. Whereas, in the case of antibiofilm activity, it has been found that AgNPs at 0.039 mg/mL, inhibit biofilms formation of Gram-negative bacteria i.e., MDR-PA, E. coli, and MDR-AB by 78.98 ± 1.12, 65.77 ± 1.05 and 66.94 ± 1.35%, respectively. On the other hand, at the same dose (i.e., 0.039 mg/mL), AP-AgNPs inhibits biofilm formation of Gram-positive bacteria i.e., MRSA, S. aureus and fungi C. albicans by 67.81 ± 0.99, 54.61 ± 1.11 and 56.22 ± 1.06%, respectively. The present work indicates the efficiency of green synthesized AP-AgNPs as good antimicrobial and antibiofilm agents against selected bacterial and fungal species.
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spelling pubmed-98222672023-01-07 Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity Ajlouni, Abdul-Wali Hamdan, Eman H. Alshalawi, Rasha Awwadh Eid Shaik, Mohammed Rafi Khan, Mujeeb Kuniyil, Mufsir Alwarthan, Abdulrahman Ansari, Mohammad Azam Khan, Merajuddin Alkhathlan, Hamad Z. Shaik, Jilani P. Adil, Syed Farooq Molecules Article Green syntheses of metallic nanoparticles using plant extracts as effective sources of reductants and stabilizers have attracted decent popularity due to their non-toxicity, environmental friendliness and rapid nature. The current study demonstrates the ecofriendly, facile and inexpensive synthesis of silver nanoparticles (AP-AgNPs) using the extract of aerial parts of the Anthemis pseudocotula Boiss. plant (AP). Herein, the aerial parts extract of AP performed a twin role of a reducing as well as a stabilizing agent. The green synthesized AP-AgNPs were characterized by several techniques such as XRD, UV-Vis, FT-IR, TEM, SEM and EDX. Furthermore, the antimicrobial and antibiofilm activity of as-prepared AP-AgNPs were examined by a standard two-fold microbroth dilution method and tissue culture plate methods, respectively, against several Gram-negative and Gram-positive bacterial strains and fungal species such as Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), multidrug-resistant Pseudomonas aeruginosa (MDR-PA) and Acinetobacter baumannii (MDR-AB), methicillin-resistant S. aureus (MRSA) and Candida albicans (C. albicans) strains. The antimicrobial activity results clearly indicated that the Gram-negative bacteria MDR-PA was most affected by AgNPs as compared to other Gram-negative and Gram-positive bacteria and fungi C. albicans. Whereas, in the case of antibiofilm activity, it has been found that AgNPs at 0.039 mg/mL, inhibit biofilms formation of Gram-negative bacteria i.e., MDR-PA, E. coli, and MDR-AB by 78.98 ± 1.12, 65.77 ± 1.05 and 66.94 ± 1.35%, respectively. On the other hand, at the same dose (i.e., 0.039 mg/mL), AP-AgNPs inhibits biofilm formation of Gram-positive bacteria i.e., MRSA, S. aureus and fungi C. albicans by 67.81 ± 0.99, 54.61 ± 1.11 and 56.22 ± 1.06%, respectively. The present work indicates the efficiency of green synthesized AP-AgNPs as good antimicrobial and antibiofilm agents against selected bacterial and fungal species. MDPI 2022-12-28 /pmc/articles/PMC9822267/ /pubmed/36615440 http://dx.doi.org/10.3390/molecules28010246 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ajlouni, Abdul-Wali
Hamdan, Eman H.
Alshalawi, Rasha Awwadh Eid
Shaik, Mohammed Rafi
Khan, Mujeeb
Kuniyil, Mufsir
Alwarthan, Abdulrahman
Ansari, Mohammad Azam
Khan, Merajuddin
Alkhathlan, Hamad Z.
Shaik, Jilani P.
Adil, Syed Farooq
Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity
title Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity
title_full Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity
title_fullStr Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity
title_full_unstemmed Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity
title_short Green Synthesis of Silver Nanoparticles Using Aerial Part Extract of the Anthemis pseudocotula Boiss. Plant and Their Biological Activity
title_sort green synthesis of silver nanoparticles using aerial part extract of the anthemis pseudocotula boiss. plant and their biological activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822267/
https://www.ncbi.nlm.nih.gov/pubmed/36615440
http://dx.doi.org/10.3390/molecules28010246
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