Cargando…
Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca(2+) Influx
Vanillin is a phenolic aldehyde, which is found in plant species of the Vanilla genus. Although recent studies have suggested that vanillin has various beneficial properties, the effect of vanillin on blood vessels has not been studied well. In the present study, we investigated whether vanillin has...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822298/ https://www.ncbi.nlm.nih.gov/pubmed/36615485 http://dx.doi.org/10.3390/molecules28010288 |
_version_ | 1784865911750000640 |
---|---|
author | Choi, Sooyeon Haam, Chae Eun Oh, Eun-Yi Byeon, Seonhee Choi, Soo-Kyoung Lee, Young-Ho |
author_facet | Choi, Sooyeon Haam, Chae Eun Oh, Eun-Yi Byeon, Seonhee Choi, Soo-Kyoung Lee, Young-Ho |
author_sort | Choi, Sooyeon |
collection | PubMed |
description | Vanillin is a phenolic aldehyde, which is found in plant species of the Vanilla genus. Although recent studies have suggested that vanillin has various beneficial properties, the effect of vanillin on blood vessels has not been studied well. In the present study, we investigated whether vanillin has vascular effects in rat mesenteric resistance arteries. To examine the vascular effect of vanillin, we measured the isometric tension of arteries using a multi-wire myograph system. After the arteries were pre-contracted with high K(+) (70 mM) or phenylephrine (5 µM), vanillin was administered. Vanillin induced concentration-dependent vasodilation. Endothelial denudation or treatment of eNOS inhibitor (L-NNA, 300 μM) did not affect the vasodilation induced by vanillin. Treatment of K(+) channel inhibitor (TEA, 10 mM) or sGC inhibitor (ODQ, 10 μM) or COX-2 inhibitor (indomethacin, 10 μM) did not affect the vanillin-induced vasodilation either. The treatment of vanillin decreased the contractile responses induced by Ca(2+) addition. Furthermore, vanillin significantly reduced vascular contraction induced by BAY K 8644 (30 nM). Vanillin induced concentration-dependent vascular relaxation in rat mesenteric resistance arteries, which was endothelium-independent. Inhibition of extracellular Ca(2+) influx was involved in vanillin-induced vasodilation. Treatment of vanillin reduced phopsho-MLC(20) in vascular smooth muscle cells. These results suggest the possibility of vanillin as a potent vasodilatory molecule. |
format | Online Article Text |
id | pubmed-9822298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98222982023-01-07 Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca(2+) Influx Choi, Sooyeon Haam, Chae Eun Oh, Eun-Yi Byeon, Seonhee Choi, Soo-Kyoung Lee, Young-Ho Molecules Article Vanillin is a phenolic aldehyde, which is found in plant species of the Vanilla genus. Although recent studies have suggested that vanillin has various beneficial properties, the effect of vanillin on blood vessels has not been studied well. In the present study, we investigated whether vanillin has vascular effects in rat mesenteric resistance arteries. To examine the vascular effect of vanillin, we measured the isometric tension of arteries using a multi-wire myograph system. After the arteries were pre-contracted with high K(+) (70 mM) or phenylephrine (5 µM), vanillin was administered. Vanillin induced concentration-dependent vasodilation. Endothelial denudation or treatment of eNOS inhibitor (L-NNA, 300 μM) did not affect the vasodilation induced by vanillin. Treatment of K(+) channel inhibitor (TEA, 10 mM) or sGC inhibitor (ODQ, 10 μM) or COX-2 inhibitor (indomethacin, 10 μM) did not affect the vanillin-induced vasodilation either. The treatment of vanillin decreased the contractile responses induced by Ca(2+) addition. Furthermore, vanillin significantly reduced vascular contraction induced by BAY K 8644 (30 nM). Vanillin induced concentration-dependent vascular relaxation in rat mesenteric resistance arteries, which was endothelium-independent. Inhibition of extracellular Ca(2+) influx was involved in vanillin-induced vasodilation. Treatment of vanillin reduced phopsho-MLC(20) in vascular smooth muscle cells. These results suggest the possibility of vanillin as a potent vasodilatory molecule. MDPI 2022-12-29 /pmc/articles/PMC9822298/ /pubmed/36615485 http://dx.doi.org/10.3390/molecules28010288 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Choi, Sooyeon Haam, Chae Eun Oh, Eun-Yi Byeon, Seonhee Choi, Soo-Kyoung Lee, Young-Ho Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca(2+) Influx |
title | Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca(2+) Influx |
title_full | Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca(2+) Influx |
title_fullStr | Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca(2+) Influx |
title_full_unstemmed | Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca(2+) Influx |
title_short | Vanillin Induces Relaxation in Rat Mesenteric Resistance Arteries by Inhibiting Extracellular Ca(2+) Influx |
title_sort | vanillin induces relaxation in rat mesenteric resistance arteries by inhibiting extracellular ca(2+) influx |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822298/ https://www.ncbi.nlm.nih.gov/pubmed/36615485 http://dx.doi.org/10.3390/molecules28010288 |
work_keys_str_mv | AT choisooyeon vanillininducesrelaxationinratmesentericresistancearteriesbyinhibitingextracellularca2influx AT haamchaeeun vanillininducesrelaxationinratmesentericresistancearteriesbyinhibitingextracellularca2influx AT oheunyi vanillininducesrelaxationinratmesentericresistancearteriesbyinhibitingextracellularca2influx AT byeonseonhee vanillininducesrelaxationinratmesentericresistancearteriesbyinhibitingextracellularca2influx AT choisookyoung vanillininducesrelaxationinratmesentericresistancearteriesbyinhibitingextracellularca2influx AT leeyoungho vanillininducesrelaxationinratmesentericresistancearteriesbyinhibitingextracellularca2influx |