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Diffraction-limited hyperspectral mid-infrared single-pixel microscopy

In this contribution, we demonstrate a wide-field hyperspectral mid-infrared (MIR) microscope based on multidimensional single-pixel imaging (SPI). The microscope employs a high brightness MIR supercontinuum source for broadband (1.55 [Formula: see text] –4.5 [Formula: see text] ) sample illuminatio...

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Autores principales: Ebner, Alexander, Gattinger, Paul, Zorin, Ivan, Krainer, Lukas, Rankl, Christian, Brandstetter, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822906/
https://www.ncbi.nlm.nih.gov/pubmed/36609672
http://dx.doi.org/10.1038/s41598-022-26718-6
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author Ebner, Alexander
Gattinger, Paul
Zorin, Ivan
Krainer, Lukas
Rankl, Christian
Brandstetter, Markus
author_facet Ebner, Alexander
Gattinger, Paul
Zorin, Ivan
Krainer, Lukas
Rankl, Christian
Brandstetter, Markus
author_sort Ebner, Alexander
collection PubMed
description In this contribution, we demonstrate a wide-field hyperspectral mid-infrared (MIR) microscope based on multidimensional single-pixel imaging (SPI). The microscope employs a high brightness MIR supercontinuum source for broadband (1.55 [Formula: see text] –4.5 [Formula: see text] ) sample illumination. Hyperspectral imaging capability is achieved by a single micro-opto-electro-mechanical digital micromirror device (DMD), which provides both spatial and spectral differentiation. For that purpose the operational spectral bandwidth of the DMD was significantly extended into the MIR spectral region. In the presented design, the DMD fulfills two essential tasks. On the one hand, as standard for the SPI approach, the DMD sequentially masks captured scenes enabling diffraction-limited imaging in the tens of millisecond time-regime. On the other hand, the diffraction at the micromirrors leads to dispersion of the projected field and thus allows for wavelength selection without the application of additional dispersive optical elements, such as gratings or prisms. In the experimental part, first of all, the imaging and spectral capabilities of the hyperspectral microscope are characterized. The spatial and spectral resolution is assessed by means of test targets and linear variable filters, respectively. At a wavelength of 4.15 [Formula: see text] a spatial resolution of 4.92 [Formula: see text] is achieved with a native spectral resolution better than 118.1 nm. Further, a post-processing method for drastic enhancement of the spectral resolution is proposed and discussed. The performance of the MIR hyperspectral microsopce is demonstrated for label-free chemical imaging and examination of polymer compounds and red blood cells. The acquisition and reconstruction of Hadamard sampled 64 [Formula: see text]  64 images is achieved in 450 ms and 162 ms, respectively. Thus, combined with an unprecedented intrinsic flexibiliy gained by a tunable field of view and adjustable spatial resolution, the demonstrated design drastically improves the sample throughput in MIR chemical and biomedical imaging.
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spelling pubmed-98229062023-01-08 Diffraction-limited hyperspectral mid-infrared single-pixel microscopy Ebner, Alexander Gattinger, Paul Zorin, Ivan Krainer, Lukas Rankl, Christian Brandstetter, Markus Sci Rep Article In this contribution, we demonstrate a wide-field hyperspectral mid-infrared (MIR) microscope based on multidimensional single-pixel imaging (SPI). The microscope employs a high brightness MIR supercontinuum source for broadband (1.55 [Formula: see text] –4.5 [Formula: see text] ) sample illumination. Hyperspectral imaging capability is achieved by a single micro-opto-electro-mechanical digital micromirror device (DMD), which provides both spatial and spectral differentiation. For that purpose the operational spectral bandwidth of the DMD was significantly extended into the MIR spectral region. In the presented design, the DMD fulfills two essential tasks. On the one hand, as standard for the SPI approach, the DMD sequentially masks captured scenes enabling diffraction-limited imaging in the tens of millisecond time-regime. On the other hand, the diffraction at the micromirrors leads to dispersion of the projected field and thus allows for wavelength selection without the application of additional dispersive optical elements, such as gratings or prisms. In the experimental part, first of all, the imaging and spectral capabilities of the hyperspectral microscope are characterized. The spatial and spectral resolution is assessed by means of test targets and linear variable filters, respectively. At a wavelength of 4.15 [Formula: see text] a spatial resolution of 4.92 [Formula: see text] is achieved with a native spectral resolution better than 118.1 nm. Further, a post-processing method for drastic enhancement of the spectral resolution is proposed and discussed. The performance of the MIR hyperspectral microsopce is demonstrated for label-free chemical imaging and examination of polymer compounds and red blood cells. The acquisition and reconstruction of Hadamard sampled 64 [Formula: see text]  64 images is achieved in 450 ms and 162 ms, respectively. Thus, combined with an unprecedented intrinsic flexibiliy gained by a tunable field of view and adjustable spatial resolution, the demonstrated design drastically improves the sample throughput in MIR chemical and biomedical imaging. Nature Publishing Group UK 2023-01-06 /pmc/articles/PMC9822906/ /pubmed/36609672 http://dx.doi.org/10.1038/s41598-022-26718-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ebner, Alexander
Gattinger, Paul
Zorin, Ivan
Krainer, Lukas
Rankl, Christian
Brandstetter, Markus
Diffraction-limited hyperspectral mid-infrared single-pixel microscopy
title Diffraction-limited hyperspectral mid-infrared single-pixel microscopy
title_full Diffraction-limited hyperspectral mid-infrared single-pixel microscopy
title_fullStr Diffraction-limited hyperspectral mid-infrared single-pixel microscopy
title_full_unstemmed Diffraction-limited hyperspectral mid-infrared single-pixel microscopy
title_short Diffraction-limited hyperspectral mid-infrared single-pixel microscopy
title_sort diffraction-limited hyperspectral mid-infrared single-pixel microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822906/
https://www.ncbi.nlm.nih.gov/pubmed/36609672
http://dx.doi.org/10.1038/s41598-022-26718-6
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