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Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue

Despite the substantial impact of skin scarring on patients and the healthcare system, there is a lack of strategies to prevent scar formation, let alone methods to remodel mature scars. Here, we took a unique approach inspired by how healthy hairbearing skin undergoes physiological remodelling duri...

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Autores principales: Plotczyk, Magdalena, Jiménez, Francisco, Limbu, Summik, Boyle, Colin J., Ovia, Jesse, Almquist, Benjamin D., Higgins, Claire A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822907/
https://www.ncbi.nlm.nih.gov/pubmed/36609660
http://dx.doi.org/10.1038/s41536-022-00270-3
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author Plotczyk, Magdalena
Jiménez, Francisco
Limbu, Summik
Boyle, Colin J.
Ovia, Jesse
Almquist, Benjamin D.
Higgins, Claire A.
author_facet Plotczyk, Magdalena
Jiménez, Francisco
Limbu, Summik
Boyle, Colin J.
Ovia, Jesse
Almquist, Benjamin D.
Higgins, Claire A.
author_sort Plotczyk, Magdalena
collection PubMed
description Despite the substantial impact of skin scarring on patients and the healthcare system, there is a lack of strategies to prevent scar formation, let alone methods to remodel mature scars. Here, we took a unique approach inspired by how healthy hairbearing skin undergoes physiological remodelling during the regular cycling of hair follicles. In this pilot clinical study, we tested if hair follicles transplanted into human scars can facilitate tissue regeneration and actively remodel fibrotic tissue, similar to how they remodel the healthy skin. We collected full-thickness skin biopsies and compared the morphology and transcriptional signature of fibrotic tissue before and after transplantation. We found that hair follicle tranplantation induced an increase in the epidermal thickness, interdigitation of the epidermal-dermal junction, dermal cell density, and blood vessel density. Remodelling of collagen type I fibres reduced the total collagen fraction, the proportion of thick fibres, and their alignment. Consistent with these morphological changes, we found a shift in the cytokine milieu of scars with a long-lasting inhibition of pro-fibrotic factors TGFβ1, IL13, and IL-6. Our results show that anagen hair follicles can attenuate the fibrotic phenotype, providing new insights for developing regenerative approaches to remodel mature scars.
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spelling pubmed-98229072023-01-08 Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue Plotczyk, Magdalena Jiménez, Francisco Limbu, Summik Boyle, Colin J. Ovia, Jesse Almquist, Benjamin D. Higgins, Claire A. NPJ Regen Med Article Despite the substantial impact of skin scarring on patients and the healthcare system, there is a lack of strategies to prevent scar formation, let alone methods to remodel mature scars. Here, we took a unique approach inspired by how healthy hairbearing skin undergoes physiological remodelling during the regular cycling of hair follicles. In this pilot clinical study, we tested if hair follicles transplanted into human scars can facilitate tissue regeneration and actively remodel fibrotic tissue, similar to how they remodel the healthy skin. We collected full-thickness skin biopsies and compared the morphology and transcriptional signature of fibrotic tissue before and after transplantation. We found that hair follicle tranplantation induced an increase in the epidermal thickness, interdigitation of the epidermal-dermal junction, dermal cell density, and blood vessel density. Remodelling of collagen type I fibres reduced the total collagen fraction, the proportion of thick fibres, and their alignment. Consistent with these morphological changes, we found a shift in the cytokine milieu of scars with a long-lasting inhibition of pro-fibrotic factors TGFβ1, IL13, and IL-6. Our results show that anagen hair follicles can attenuate the fibrotic phenotype, providing new insights for developing regenerative approaches to remodel mature scars. Nature Publishing Group UK 2023-01-06 /pmc/articles/PMC9822907/ /pubmed/36609660 http://dx.doi.org/10.1038/s41536-022-00270-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Plotczyk, Magdalena
Jiménez, Francisco
Limbu, Summik
Boyle, Colin J.
Ovia, Jesse
Almquist, Benjamin D.
Higgins, Claire A.
Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue
title Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue
title_full Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue
title_fullStr Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue
title_full_unstemmed Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue
title_short Anagen hair follicles transplanted into mature human scars remodel fibrotic tissue
title_sort anagen hair follicles transplanted into mature human scars remodel fibrotic tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822907/
https://www.ncbi.nlm.nih.gov/pubmed/36609660
http://dx.doi.org/10.1038/s41536-022-00270-3
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