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Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle
Given its limited accessibility, the CA2 area has been less investigated compared to other subregions of the hippocampus. While the development of transgenic mice expressing Cre recombinase in the CA2 has revealed unique features of this area, the use of mouse lines has several limitations, such as...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822962/ https://www.ncbi.nlm.nih.gov/pubmed/36609635 http://dx.doi.org/10.1038/s41598-022-27372-8 |
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author | Okamoto, Kazuki Kamikubo, Yuji Yamauchi, Kenta Okamoto, Shinichiro Takahashi, Megumu Ishida, Yoko Koike, Masato Ikegaya, Yuji Sakurai, Takashi Hioki, Hiroyuki |
author_facet | Okamoto, Kazuki Kamikubo, Yuji Yamauchi, Kenta Okamoto, Shinichiro Takahashi, Megumu Ishida, Yoko Koike, Masato Ikegaya, Yuji Sakurai, Takashi Hioki, Hiroyuki |
author_sort | Okamoto, Kazuki |
collection | PubMed |
description | Given its limited accessibility, the CA2 area has been less investigated compared to other subregions of the hippocampus. While the development of transgenic mice expressing Cre recombinase in the CA2 has revealed unique features of this area, the use of mouse lines has several limitations, such as lack of specificity. Therefore, a specific gene delivery system is required. Here, we confirmed that the AAV-PHP.eB capsid preferably infected CA2 pyramidal cells following retro-orbital injection and demonstrated that the specificity was substantially higher after injection into the lateral ventricle. In addition, a tropism for the CA2 area was observed in organotypic slice cultures. Combined injection into the lateral ventricle and stereotaxic injection into the CA2 area specifically introduced the transgene into CA2 pyramidal cells, enabling us to perform targeted patch-clamp recordings and optogenetic manipulation. These results suggest that AAV-PHP.eB is a versatile tool for specific gene transduction in CA2 pyramidal cells. |
format | Online Article Text |
id | pubmed-9822962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-98229622023-01-08 Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle Okamoto, Kazuki Kamikubo, Yuji Yamauchi, Kenta Okamoto, Shinichiro Takahashi, Megumu Ishida, Yoko Koike, Masato Ikegaya, Yuji Sakurai, Takashi Hioki, Hiroyuki Sci Rep Article Given its limited accessibility, the CA2 area has been less investigated compared to other subregions of the hippocampus. While the development of transgenic mice expressing Cre recombinase in the CA2 has revealed unique features of this area, the use of mouse lines has several limitations, such as lack of specificity. Therefore, a specific gene delivery system is required. Here, we confirmed that the AAV-PHP.eB capsid preferably infected CA2 pyramidal cells following retro-orbital injection and demonstrated that the specificity was substantially higher after injection into the lateral ventricle. In addition, a tropism for the CA2 area was observed in organotypic slice cultures. Combined injection into the lateral ventricle and stereotaxic injection into the CA2 area specifically introduced the transgene into CA2 pyramidal cells, enabling us to perform targeted patch-clamp recordings and optogenetic manipulation. These results suggest that AAV-PHP.eB is a versatile tool for specific gene transduction in CA2 pyramidal cells. Nature Publishing Group UK 2023-01-06 /pmc/articles/PMC9822962/ /pubmed/36609635 http://dx.doi.org/10.1038/s41598-022-27372-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Okamoto, Kazuki Kamikubo, Yuji Yamauchi, Kenta Okamoto, Shinichiro Takahashi, Megumu Ishida, Yoko Koike, Masato Ikegaya, Yuji Sakurai, Takashi Hioki, Hiroyuki Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
title | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
title_full | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
title_fullStr | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
title_full_unstemmed | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
title_short | Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle |
title_sort | specific aav2/php.eb-mediated gene transduction of ca2 pyramidal cells via injection into the lateral ventricle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9822962/ https://www.ncbi.nlm.nih.gov/pubmed/36609635 http://dx.doi.org/10.1038/s41598-022-27372-8 |
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