Cargando…

The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation

Proliferation, metabolism, and migration of hematopoietic stem/progenitor cells (HSPCs) are coordinated by receptors expressed on outer cell membranes that are integrated into microdomains, known as membrane lipid rafts (MLRs). These structures float freely in the cell membrane bilayer and are enric...

Descripción completa

Detalles Bibliográficos
Autores principales: Abdelbaset-Ismail, Ahmed, Ciechanowicz, Andrzej K., Bujko, Kamila, Ratajczak, Janina, Kucia, Magdalena, Ratajczak, Mariusz Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823029/
https://www.ncbi.nlm.nih.gov/pubmed/36441489
http://dx.doi.org/10.1007/s12015-022-10481-2
_version_ 1784866066348900352
author Abdelbaset-Ismail, Ahmed
Ciechanowicz, Andrzej K.
Bujko, Kamila
Ratajczak, Janina
Kucia, Magdalena
Ratajczak, Mariusz Z.
author_facet Abdelbaset-Ismail, Ahmed
Ciechanowicz, Andrzej K.
Bujko, Kamila
Ratajczak, Janina
Kucia, Magdalena
Ratajczak, Mariusz Z.
author_sort Abdelbaset-Ismail, Ahmed
collection PubMed
description Proliferation, metabolism, and migration of hematopoietic stem/progenitor cells (HSPCs) are coordinated by receptors expressed on outer cell membranes that are integrated into microdomains, known as membrane lipid rafts (MLRs). These structures float freely in the cell membrane bilayer and are enriched in cholesterol and sphingolipids for their functional integrity. Receptors, if expressed in MLRs, have prolonged occupancy on the cell surface and enhanced signaling power. Based on this, we have become interested in the regulation of synthesis of MLRs components in HSPCs. To address this, we tested the effect of selected factors that promote proliferation or migration and their potential involvement in the synthesis of MLRs components in HSPCs. Based on our previous research showing that HSPCs from Nox2-KO and Nlrp3-KO mice display a profound defect in MLRs formation, we focused on the role of Nox2-ROS-Nlrp3 inflammasome in regulating lipogenesis in HSPCs. We found that while at steady state conditions, Nox2-derived ROS is required for a proper expression of enzymes regulating lipogenesis, during inflammation, this effect is augmented by Nlrp3 inflammasome. Thus, our data sheds new light on the regulation of lipogenesis in HSPCs and the involvement of the Nox2-ROS-Nlrp3 inflammasome axis that differently regulates lipogenesis at steady state conditions and in response to inflammation, modulating MLRs-mediated responsiveness of these cells to external stimuli. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12015-022-10481-2.
format Online
Article
Text
id pubmed-9823029
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-98230292023-01-08 The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation Abdelbaset-Ismail, Ahmed Ciechanowicz, Andrzej K. Bujko, Kamila Ratajczak, Janina Kucia, Magdalena Ratajczak, Mariusz Z. Stem Cell Rev Rep Article Proliferation, metabolism, and migration of hematopoietic stem/progenitor cells (HSPCs) are coordinated by receptors expressed on outer cell membranes that are integrated into microdomains, known as membrane lipid rafts (MLRs). These structures float freely in the cell membrane bilayer and are enriched in cholesterol and sphingolipids for their functional integrity. Receptors, if expressed in MLRs, have prolonged occupancy on the cell surface and enhanced signaling power. Based on this, we have become interested in the regulation of synthesis of MLRs components in HSPCs. To address this, we tested the effect of selected factors that promote proliferation or migration and their potential involvement in the synthesis of MLRs components in HSPCs. Based on our previous research showing that HSPCs from Nox2-KO and Nlrp3-KO mice display a profound defect in MLRs formation, we focused on the role of Nox2-ROS-Nlrp3 inflammasome in regulating lipogenesis in HSPCs. We found that while at steady state conditions, Nox2-derived ROS is required for a proper expression of enzymes regulating lipogenesis, during inflammation, this effect is augmented by Nlrp3 inflammasome. Thus, our data sheds new light on the regulation of lipogenesis in HSPCs and the involvement of the Nox2-ROS-Nlrp3 inflammasome axis that differently regulates lipogenesis at steady state conditions and in response to inflammation, modulating MLRs-mediated responsiveness of these cells to external stimuli. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12015-022-10481-2. Springer US 2022-11-28 2023 /pmc/articles/PMC9823029/ /pubmed/36441489 http://dx.doi.org/10.1007/s12015-022-10481-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Abdelbaset-Ismail, Ahmed
Ciechanowicz, Andrzej K.
Bujko, Kamila
Ratajczak, Janina
Kucia, Magdalena
Ratajczak, Mariusz Z.
The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation
title The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation
title_full The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation
title_fullStr The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation
title_full_unstemmed The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation
title_short The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation
title_sort nox2-ros-nlrp3 inflammasome signaling stimulates in the hematopoietic stem/progenitor cells lipogenesis to facilitate membrane lipid raft formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823029/
https://www.ncbi.nlm.nih.gov/pubmed/36441489
http://dx.doi.org/10.1007/s12015-022-10481-2
work_keys_str_mv AT abdelbasetismailahmed thenox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT ciechanowiczandrzejk thenox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT bujkokamila thenox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT ratajczakjanina thenox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT kuciamagdalena thenox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT ratajczakmariuszz thenox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT abdelbasetismailahmed nox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT ciechanowiczandrzejk nox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT bujkokamila nox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT ratajczakjanina nox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT kuciamagdalena nox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation
AT ratajczakmariuszz nox2rosnlrp3inflammasomesignalingstimulatesinthehematopoieticstemprogenitorcellslipogenesistofacilitatemembranelipidraftformation