Cargando…

Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves

The effect of salt stress on the expression of genes, the methylation of their promoters, and the enzymatic activity of succinate dehydrogenase (SDH) and succinic semialdehyde dehydrogenase (SSADH) was investigated in maize (Zea mays L.). The incubation of maize seedlings in a 150 mM NaCl solution f...

Descripción completa

Detalles Bibliográficos
Autores principales: Fedorin, Dmitry N., Eprintsev, Alexander T., Florez Caro, Orlando J., Igamberdiev, Abir U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823291/
https://www.ncbi.nlm.nih.gov/pubmed/36616197
http://dx.doi.org/10.3390/plants12010068
_version_ 1784866123582275584
author Fedorin, Dmitry N.
Eprintsev, Alexander T.
Florez Caro, Orlando J.
Igamberdiev, Abir U.
author_facet Fedorin, Dmitry N.
Eprintsev, Alexander T.
Florez Caro, Orlando J.
Igamberdiev, Abir U.
author_sort Fedorin, Dmitry N.
collection PubMed
description The effect of salt stress on the expression of genes, the methylation of their promoters, and the enzymatic activity of succinate dehydrogenase (SDH) and succinic semialdehyde dehydrogenase (SSADH) was investigated in maize (Zea mays L.). The incubation of maize seedlings in a 150 mM NaCl solution for 24 h led to a several-fold increase in the activity of SSADH that peaked at 6 h of NaCl treatment, which was preceded by an increase in the Ssadh1 gene expression and a decrease in its promoter methylation observed at 3 h of salt stress. The increase in SDH activity and succinate oxidation by mitochondria was slower, developing by 24 h of NaCl treatment, which corresponded to the increase in expression of the genes Sdh1-2 and Sdh2-3 encoding SDH catalytic subunits and of the gene Sdh3-1 encoding the anchoring SDH subunit. The increase in the Sdh2-3 expression was accompanied by the decrease in promoter methylation. It is concluded that salt stress results in the rapid increase in succinate production via SSADH operating in the GABA shunt, which leads to the activation of SDH, the process partially regulated via epigenetic mechanisms. The role of succinate metabolism under the conditions of salt stress is discussed.
format Online
Article
Text
id pubmed-9823291
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98232912023-01-08 Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves Fedorin, Dmitry N. Eprintsev, Alexander T. Florez Caro, Orlando J. Igamberdiev, Abir U. Plants (Basel) Article The effect of salt stress on the expression of genes, the methylation of their promoters, and the enzymatic activity of succinate dehydrogenase (SDH) and succinic semialdehyde dehydrogenase (SSADH) was investigated in maize (Zea mays L.). The incubation of maize seedlings in a 150 mM NaCl solution for 24 h led to a several-fold increase in the activity of SSADH that peaked at 6 h of NaCl treatment, which was preceded by an increase in the Ssadh1 gene expression and a decrease in its promoter methylation observed at 3 h of salt stress. The increase in SDH activity and succinate oxidation by mitochondria was slower, developing by 24 h of NaCl treatment, which corresponded to the increase in expression of the genes Sdh1-2 and Sdh2-3 encoding SDH catalytic subunits and of the gene Sdh3-1 encoding the anchoring SDH subunit. The increase in the Sdh2-3 expression was accompanied by the decrease in promoter methylation. It is concluded that salt stress results in the rapid increase in succinate production via SSADH operating in the GABA shunt, which leads to the activation of SDH, the process partially regulated via epigenetic mechanisms. The role of succinate metabolism under the conditions of salt stress is discussed. MDPI 2022-12-23 /pmc/articles/PMC9823291/ /pubmed/36616197 http://dx.doi.org/10.3390/plants12010068 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fedorin, Dmitry N.
Eprintsev, Alexander T.
Florez Caro, Orlando J.
Igamberdiev, Abir U.
Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves
title Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves
title_full Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves
title_fullStr Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves
title_full_unstemmed Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves
title_short Effect of Salt Stress on the Activity, Expression, and Promoter Methylation of Succinate Dehydrogenase and Succinic Semialdehyde Dehydrogenase in Maize (Zea mays L.) Leaves
title_sort effect of salt stress on the activity, expression, and promoter methylation of succinate dehydrogenase and succinic semialdehyde dehydrogenase in maize (zea mays l.) leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823291/
https://www.ncbi.nlm.nih.gov/pubmed/36616197
http://dx.doi.org/10.3390/plants12010068
work_keys_str_mv AT fedorindmitryn effectofsaltstressontheactivityexpressionandpromotermethylationofsuccinatedehydrogenaseandsuccinicsemialdehydedehydrogenaseinmaizezeamayslleaves
AT eprintsevalexandert effectofsaltstressontheactivityexpressionandpromotermethylationofsuccinatedehydrogenaseandsuccinicsemialdehydedehydrogenaseinmaizezeamayslleaves
AT florezcaroorlandoj effectofsaltstressontheactivityexpressionandpromotermethylationofsuccinatedehydrogenaseandsuccinicsemialdehydedehydrogenaseinmaizezeamayslleaves
AT igamberdievabiru effectofsaltstressontheactivityexpressionandpromotermethylationofsuccinatedehydrogenaseandsuccinicsemialdehydedehydrogenaseinmaizezeamayslleaves