Cargando…
Antibacterial, Antioxidant Activities, GC-Mass Characterization, and Cyto/Genotoxicity Effect of Green Synthesis of Silver Nanoparticles Using Latex of Cynanchum acutum L
Green synthesis of nanoparticles is receiving more attention these days since it is simple to use and prepare, uses fewer harsh chemicals and chemical reactions, and is environmentally benign. A novel strategy aims to recycle poisonous plant chemicals and use them as natural stabilizing capping agen...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823559/ https://www.ncbi.nlm.nih.gov/pubmed/36616301 http://dx.doi.org/10.3390/plants12010172 |
_version_ | 1784866189557628928 |
---|---|
author | Soliman, Magda I. Mohammed, Nada S. EL-Sherbeny, Ghada Safhi, Fatmah Ahmed ALshamrani, Salha Mesfer Alyamani, Amal A. Alharthi, Badr Qahl, Safa H. Al Kashgry, Najla Amin T. Abd-Ellatif, Sawsan Ibrahim, Amira A. |
author_facet | Soliman, Magda I. Mohammed, Nada S. EL-Sherbeny, Ghada Safhi, Fatmah Ahmed ALshamrani, Salha Mesfer Alyamani, Amal A. Alharthi, Badr Qahl, Safa H. Al Kashgry, Najla Amin T. Abd-Ellatif, Sawsan Ibrahim, Amira A. |
author_sort | Soliman, Magda I. |
collection | PubMed |
description | Green synthesis of nanoparticles is receiving more attention these days since it is simple to use and prepare, uses fewer harsh chemicals and chemical reactions, and is environmentally benign. A novel strategy aims to recycle poisonous plant chemicals and use them as natural stabilizing capping agents for nanoparticles. In this investigation, silver nanoparticles loaded with latex from Cynanchum acutum L. (Cy-AgNPs) were examined using a transmission electron microscope, FT-IR spectroscopy, and UV-visible spectroscopy. Additionally, using Vicia faba as a model test plant, the genotoxicity and cytotoxicity effects of crude latex and various concentrations of Cy-AgNPs were studied. The majority of the particles were spherical in shape. The highest antioxidant activity using DPPH was illustrated for CAgNPs (25 mg/L) (70.26 ± 1.32%) and decreased with increased concentrations of Cy-AGNPs. Antibacterial activity for all treatments was determined showing that the highest antibacterial activity was for Cy-AgNPs (50 mg/L) with inhibition zone 24 ± 0.014 mm against Bacillus subtilis, 19 ± 0.12 mm against Escherichia coli, and 23 ± 0.015 against Staphylococcus aureus. For phytochemical analysis, the highest levels of secondary metabolites from phenolic content, flavonoids, tannins, and alkaloids, were found in Cy-AgNPs (25 mg/L). Vicia faba treated with Cy-AgNPs- (25 mg/L) displayed the highest mitotic index (MI%) value of 9.08% compared to other Cy-AgNP concentrations (50–100 mg/L) and C. acutum crude latex concentrations (3%). To detect cytotoxicity, a variety of chromosomal abnormalities were used, including micronuclei at interphase, disturbed at metaphase and anaphase, chromosomal stickiness, bridges, and laggards. The concentration of Cy-AgNPs (25 mg/L) had the lowest level of chromosomal aberrations, with a value of 23.41% versus 20.81% for the control. Proteins from seeds treated with V. faba produced sixteen bands on SDS-PAGE, comprising ten monomorphic bands and six polymorphic bands, for a total percentage of polymorphism of 37.5%. Eight ISSR primers were employed to generate a total of 79 bands, 56 of which were polymorphic and 23 of which were common. Primer ISSR 14 has the highest level of polymorphism (92.86%), according to the data. Using biochemical SDS-PAGE and ISSR molecular markers, Cy-AgNPs (25 mg/L) showed the highest percentage of genomic template stability (GTS%), with values of 80% and 51.28%, respectively. The findings of this work suggest employing CyAgNPs (25 mg/L) in pharmaceutical purposes due to its highest content of bioactive compounds and lowest concentration of chromosomal abnormalities. |
format | Online Article Text |
id | pubmed-9823559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98235592023-01-08 Antibacterial, Antioxidant Activities, GC-Mass Characterization, and Cyto/Genotoxicity Effect of Green Synthesis of Silver Nanoparticles Using Latex of Cynanchum acutum L Soliman, Magda I. Mohammed, Nada S. EL-Sherbeny, Ghada Safhi, Fatmah Ahmed ALshamrani, Salha Mesfer Alyamani, Amal A. Alharthi, Badr Qahl, Safa H. Al Kashgry, Najla Amin T. Abd-Ellatif, Sawsan Ibrahim, Amira A. Plants (Basel) Article Green synthesis of nanoparticles is receiving more attention these days since it is simple to use and prepare, uses fewer harsh chemicals and chemical reactions, and is environmentally benign. A novel strategy aims to recycle poisonous plant chemicals and use them as natural stabilizing capping agents for nanoparticles. In this investigation, silver nanoparticles loaded with latex from Cynanchum acutum L. (Cy-AgNPs) were examined using a transmission electron microscope, FT-IR spectroscopy, and UV-visible spectroscopy. Additionally, using Vicia faba as a model test plant, the genotoxicity and cytotoxicity effects of crude latex and various concentrations of Cy-AgNPs were studied. The majority of the particles were spherical in shape. The highest antioxidant activity using DPPH was illustrated for CAgNPs (25 mg/L) (70.26 ± 1.32%) and decreased with increased concentrations of Cy-AGNPs. Antibacterial activity for all treatments was determined showing that the highest antibacterial activity was for Cy-AgNPs (50 mg/L) with inhibition zone 24 ± 0.014 mm against Bacillus subtilis, 19 ± 0.12 mm against Escherichia coli, and 23 ± 0.015 against Staphylococcus aureus. For phytochemical analysis, the highest levels of secondary metabolites from phenolic content, flavonoids, tannins, and alkaloids, were found in Cy-AgNPs (25 mg/L). Vicia faba treated with Cy-AgNPs- (25 mg/L) displayed the highest mitotic index (MI%) value of 9.08% compared to other Cy-AgNP concentrations (50–100 mg/L) and C. acutum crude latex concentrations (3%). To detect cytotoxicity, a variety of chromosomal abnormalities were used, including micronuclei at interphase, disturbed at metaphase and anaphase, chromosomal stickiness, bridges, and laggards. The concentration of Cy-AgNPs (25 mg/L) had the lowest level of chromosomal aberrations, with a value of 23.41% versus 20.81% for the control. Proteins from seeds treated with V. faba produced sixteen bands on SDS-PAGE, comprising ten monomorphic bands and six polymorphic bands, for a total percentage of polymorphism of 37.5%. Eight ISSR primers were employed to generate a total of 79 bands, 56 of which were polymorphic and 23 of which were common. Primer ISSR 14 has the highest level of polymorphism (92.86%), according to the data. Using biochemical SDS-PAGE and ISSR molecular markers, Cy-AgNPs (25 mg/L) showed the highest percentage of genomic template stability (GTS%), with values of 80% and 51.28%, respectively. The findings of this work suggest employing CyAgNPs (25 mg/L) in pharmaceutical purposes due to its highest content of bioactive compounds and lowest concentration of chromosomal abnormalities. MDPI 2022-12-30 /pmc/articles/PMC9823559/ /pubmed/36616301 http://dx.doi.org/10.3390/plants12010172 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Soliman, Magda I. Mohammed, Nada S. EL-Sherbeny, Ghada Safhi, Fatmah Ahmed ALshamrani, Salha Mesfer Alyamani, Amal A. Alharthi, Badr Qahl, Safa H. Al Kashgry, Najla Amin T. Abd-Ellatif, Sawsan Ibrahim, Amira A. Antibacterial, Antioxidant Activities, GC-Mass Characterization, and Cyto/Genotoxicity Effect of Green Synthesis of Silver Nanoparticles Using Latex of Cynanchum acutum L |
title | Antibacterial, Antioxidant Activities, GC-Mass Characterization, and Cyto/Genotoxicity Effect of Green Synthesis of Silver Nanoparticles Using Latex of Cynanchum acutum L |
title_full | Antibacterial, Antioxidant Activities, GC-Mass Characterization, and Cyto/Genotoxicity Effect of Green Synthesis of Silver Nanoparticles Using Latex of Cynanchum acutum L |
title_fullStr | Antibacterial, Antioxidant Activities, GC-Mass Characterization, and Cyto/Genotoxicity Effect of Green Synthesis of Silver Nanoparticles Using Latex of Cynanchum acutum L |
title_full_unstemmed | Antibacterial, Antioxidant Activities, GC-Mass Characterization, and Cyto/Genotoxicity Effect of Green Synthesis of Silver Nanoparticles Using Latex of Cynanchum acutum L |
title_short | Antibacterial, Antioxidant Activities, GC-Mass Characterization, and Cyto/Genotoxicity Effect of Green Synthesis of Silver Nanoparticles Using Latex of Cynanchum acutum L |
title_sort | antibacterial, antioxidant activities, gc-mass characterization, and cyto/genotoxicity effect of green synthesis of silver nanoparticles using latex of cynanchum acutum l |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823559/ https://www.ncbi.nlm.nih.gov/pubmed/36616301 http://dx.doi.org/10.3390/plants12010172 |
work_keys_str_mv | AT solimanmagdai antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT mohammednadas antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT elsherbenyghada antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT safhifatmahahmed antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT alshamranisalhamesfer antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT alyamaniamala antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT alharthibadr antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT qahlsafah antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT alkashgrynajlaamint antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT abdellatifsawsan antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml AT ibrahimamiraa antibacterialantioxidantactivitiesgcmasscharacterizationandcytogenotoxicityeffectofgreensynthesisofsilvernanoparticlesusinglatexofcynanchumacutuml |