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Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer

The production of heat by mitochondria is critical for maintaining body temperature, regulating metabolic rate, and preventing oxidative damage to mitochondria and cells. Until the present, mitochondrial heat production has been characterized only by methods based on fluorescent probes, which are se...

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Autores principales: Romshin, Alexey M., Osypov, Alexander A., Popova, Irina Yu., Zeeb, Vadim E., Sinogeykin, Andrey G., Vlasov, Igor I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823591/
https://www.ncbi.nlm.nih.gov/pubmed/36616008
http://dx.doi.org/10.3390/nano13010098
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author Romshin, Alexey M.
Osypov, Alexander A.
Popova, Irina Yu.
Zeeb, Vadim E.
Sinogeykin, Andrey G.
Vlasov, Igor I.
author_facet Romshin, Alexey M.
Osypov, Alexander A.
Popova, Irina Yu.
Zeeb, Vadim E.
Sinogeykin, Andrey G.
Vlasov, Igor I.
author_sort Romshin, Alexey M.
collection PubMed
description The production of heat by mitochondria is critical for maintaining body temperature, regulating metabolic rate, and preventing oxidative damage to mitochondria and cells. Until the present, mitochondrial heat production has been characterized only by methods based on fluorescent probes, which are sensitive to environmental variations (viscosity, pH, ionic strength, quenching, etc.). Here, for the first time, the heat release of isolated mitochondria was unambiguously measured by a diamond thermometer (DT), which is absolutely indifferent to external non-thermal parameters. We show that during total uncoupling of transmembrane potential by CCCP application, the temperature near the mitochondria rises by 4–22 °C above the ambient temperature with an absolute maximum of 45 °C. Such a broad variation in the temperature response is associated with the heterogeneity of the mitochondria themselves as well as their aggregations in the isolated suspension. Spontaneous temperature bursts with comparable amplitude were also detected prior to CCCP application, which may reflect involvement of some mitochondria to ATP synthesis or membrane potential leaking to avoid hyperproduction of reactive oxygen species. The results obtained with the diamond temperature sensor shed light on the “hot mitochondria” paradox.
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spelling pubmed-98235912023-01-08 Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer Romshin, Alexey M. Osypov, Alexander A. Popova, Irina Yu. Zeeb, Vadim E. Sinogeykin, Andrey G. Vlasov, Igor I. Nanomaterials (Basel) Article The production of heat by mitochondria is critical for maintaining body temperature, regulating metabolic rate, and preventing oxidative damage to mitochondria and cells. Until the present, mitochondrial heat production has been characterized only by methods based on fluorescent probes, which are sensitive to environmental variations (viscosity, pH, ionic strength, quenching, etc.). Here, for the first time, the heat release of isolated mitochondria was unambiguously measured by a diamond thermometer (DT), which is absolutely indifferent to external non-thermal parameters. We show that during total uncoupling of transmembrane potential by CCCP application, the temperature near the mitochondria rises by 4–22 °C above the ambient temperature with an absolute maximum of 45 °C. Such a broad variation in the temperature response is associated with the heterogeneity of the mitochondria themselves as well as their aggregations in the isolated suspension. Spontaneous temperature bursts with comparable amplitude were also detected prior to CCCP application, which may reflect involvement of some mitochondria to ATP synthesis or membrane potential leaking to avoid hyperproduction of reactive oxygen species. The results obtained with the diamond temperature sensor shed light on the “hot mitochondria” paradox. MDPI 2022-12-25 /pmc/articles/PMC9823591/ /pubmed/36616008 http://dx.doi.org/10.3390/nano13010098 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Romshin, Alexey M.
Osypov, Alexander A.
Popova, Irina Yu.
Zeeb, Vadim E.
Sinogeykin, Andrey G.
Vlasov, Igor I.
Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer
title Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer
title_full Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer
title_fullStr Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer
title_full_unstemmed Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer
title_short Heat Release by Isolated Mouse Brain Mitochondria Detected with Diamond Thermometer
title_sort heat release by isolated mouse brain mitochondria detected with diamond thermometer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823591/
https://www.ncbi.nlm.nih.gov/pubmed/36616008
http://dx.doi.org/10.3390/nano13010098
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