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Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato

As a cold-sensitive species, tomato is frequently challenged by cold stress during vegetative and reproductive growth. Understanding how tomato responds to cold stress is of critical importance for sustainable tomato production. In this work, we demonstrate that jasmonate (JA) plays a crucial role i...

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Detalles Bibliográficos
Autores principales: Ding, Fei, Wang, Xizhi, Li, Ziye, Wang, Meiling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823970/
https://www.ncbi.nlm.nih.gov/pubmed/36616188
http://dx.doi.org/10.3390/plants12010060
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author Ding, Fei
Wang, Xizhi
Li, Ziye
Wang, Meiling
author_facet Ding, Fei
Wang, Xizhi
Li, Ziye
Wang, Meiling
author_sort Ding, Fei
collection PubMed
description As a cold-sensitive species, tomato is frequently challenged by cold stress during vegetative and reproductive growth. Understanding how tomato responds to cold stress is of critical importance for sustainable tomato production. In this work, we demonstrate that jasmonate (JA) plays a crucial role in tomato response to cold stress by promoting abscisic acid (ABA) biosynthesis. It was observed that both JA and ABA levels were substantially increased under cold conditions, whereas the suppression of JA biosynthesis abated ABA accumulation. The ABA biosynthesis gene 9-CIS-EPOXYCAROTENOID DIOXYGENASE2 (NCED2) was subsequently found to be associated with JA-mediated ABA biosynthesis in tomato plants in response to cold stress. NCED2 was rapidly induced by exogenous MeJA and cold treatment. Silencing NCED2 led to a decrease in ABA accumulation that was concurrent with increased cold sensitivity. Moreover, blocking ABA biosynthesis using a chemical inhibitor impaired JA-induced cold tolerance in tomato. Furthermore, MYC2, a core component of the JA signaling pathway, promoted the transcription of NCED2, ABA accumulation and cold tolerance in tomato. Collectively, our results support that JA signaling promotes ABA biosynthesis to confer cold tolerance in tomato.
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spelling pubmed-98239702023-01-08 Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato Ding, Fei Wang, Xizhi Li, Ziye Wang, Meiling Plants (Basel) Article As a cold-sensitive species, tomato is frequently challenged by cold stress during vegetative and reproductive growth. Understanding how tomato responds to cold stress is of critical importance for sustainable tomato production. In this work, we demonstrate that jasmonate (JA) plays a crucial role in tomato response to cold stress by promoting abscisic acid (ABA) biosynthesis. It was observed that both JA and ABA levels were substantially increased under cold conditions, whereas the suppression of JA biosynthesis abated ABA accumulation. The ABA biosynthesis gene 9-CIS-EPOXYCAROTENOID DIOXYGENASE2 (NCED2) was subsequently found to be associated with JA-mediated ABA biosynthesis in tomato plants in response to cold stress. NCED2 was rapidly induced by exogenous MeJA and cold treatment. Silencing NCED2 led to a decrease in ABA accumulation that was concurrent with increased cold sensitivity. Moreover, blocking ABA biosynthesis using a chemical inhibitor impaired JA-induced cold tolerance in tomato. Furthermore, MYC2, a core component of the JA signaling pathway, promoted the transcription of NCED2, ABA accumulation and cold tolerance in tomato. Collectively, our results support that JA signaling promotes ABA biosynthesis to confer cold tolerance in tomato. MDPI 2022-12-22 /pmc/articles/PMC9823970/ /pubmed/36616188 http://dx.doi.org/10.3390/plants12010060 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ding, Fei
Wang, Xizhi
Li, Ziye
Wang, Meiling
Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato
title Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato
title_full Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato
title_fullStr Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato
title_full_unstemmed Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato
title_short Jasmonate Positively Regulates Cold Tolerance by Promoting ABA Biosynthesis in Tomato
title_sort jasmonate positively regulates cold tolerance by promoting aba biosynthesis in tomato
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823970/
https://www.ncbi.nlm.nih.gov/pubmed/36616188
http://dx.doi.org/10.3390/plants12010060
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