Cargando…

Molecular Analysis of Genetic Diversity and Structure of the Lablab (Lablab purpureus (L.) Sweet) Gene Pool Reveals Two Independent Routes of Domestication

In this study, genetic diversity and structure of 474 cultivated and 19 wild lablab (Lablab purpureus) accessions. were determined using 15 nuclear and 6 chloroplast SSR markers. The overall gene diversity was relatively low (0.3441). Gene diversity in the wild accessions (0.6059) was about two-fold...

Descripción completa

Detalles Bibliográficos
Autores principales: Kongjaimun, Alisa, Takahashi, Yu, Yoshioka, Yosuke, Tomooka, Norihiko, Mongkol, Rachsawan, Somta, Prakit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824144/
https://www.ncbi.nlm.nih.gov/pubmed/36616186
http://dx.doi.org/10.3390/plants12010057
_version_ 1784866337038794752
author Kongjaimun, Alisa
Takahashi, Yu
Yoshioka, Yosuke
Tomooka, Norihiko
Mongkol, Rachsawan
Somta, Prakit
author_facet Kongjaimun, Alisa
Takahashi, Yu
Yoshioka, Yosuke
Tomooka, Norihiko
Mongkol, Rachsawan
Somta, Prakit
author_sort Kongjaimun, Alisa
collection PubMed
description In this study, genetic diversity and structure of 474 cultivated and 19 wild lablab (Lablab purpureus) accessions. were determined using 15 nuclear and 6 chloroplast SSR markers. The overall gene diversity was relatively low (0.3441). Gene diversity in the wild accessions (0.6059) was about two-folds greater than that in the cultivated accessions. In the wild accessions, gene diversity was greatest in the southern Africa, followed by East Africa. In the cultivated accessions, gene diversity was highest in the eastern Africa. The results suggested that South Africa is the center of origin and East Africa is the center of domestication of lablab. Different cluster analyses showed that 2-seeded-pod cultivated accessions (ssp. uncinatus) were clustered with wild accessions and that 4–(6)-seeded-pod cultivated accessions (ssp. purpureus and bengalensis) were intermingled. UPGMA tree suggested that ssp. purpureus and bengalensis were domesticated from 4-seeded-pod wild accessions of southern Africa. Haplotype network analysis based on nuclear SSRs revealed two domestication routes; the ssp. uncinatus is domesticated from 2-seeded-pod wild lablab (wild spp. uncinatus) from East Africa (Ethiopia), while the ssp. purpureus and bengalensis are domesticated from 4-seeded-pod wild lablab from Central Africa (Rwanda). These results are useful for understanding domestication and revising classification of lablab.
format Online
Article
Text
id pubmed-9824144
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98241442023-01-08 Molecular Analysis of Genetic Diversity and Structure of the Lablab (Lablab purpureus (L.) Sweet) Gene Pool Reveals Two Independent Routes of Domestication Kongjaimun, Alisa Takahashi, Yu Yoshioka, Yosuke Tomooka, Norihiko Mongkol, Rachsawan Somta, Prakit Plants (Basel) Article In this study, genetic diversity and structure of 474 cultivated and 19 wild lablab (Lablab purpureus) accessions. were determined using 15 nuclear and 6 chloroplast SSR markers. The overall gene diversity was relatively low (0.3441). Gene diversity in the wild accessions (0.6059) was about two-folds greater than that in the cultivated accessions. In the wild accessions, gene diversity was greatest in the southern Africa, followed by East Africa. In the cultivated accessions, gene diversity was highest in the eastern Africa. The results suggested that South Africa is the center of origin and East Africa is the center of domestication of lablab. Different cluster analyses showed that 2-seeded-pod cultivated accessions (ssp. uncinatus) were clustered with wild accessions and that 4–(6)-seeded-pod cultivated accessions (ssp. purpureus and bengalensis) were intermingled. UPGMA tree suggested that ssp. purpureus and bengalensis were domesticated from 4-seeded-pod wild accessions of southern Africa. Haplotype network analysis based on nuclear SSRs revealed two domestication routes; the ssp. uncinatus is domesticated from 2-seeded-pod wild lablab (wild spp. uncinatus) from East Africa (Ethiopia), while the ssp. purpureus and bengalensis are domesticated from 4-seeded-pod wild lablab from Central Africa (Rwanda). These results are useful for understanding domestication and revising classification of lablab. MDPI 2022-12-22 /pmc/articles/PMC9824144/ /pubmed/36616186 http://dx.doi.org/10.3390/plants12010057 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kongjaimun, Alisa
Takahashi, Yu
Yoshioka, Yosuke
Tomooka, Norihiko
Mongkol, Rachsawan
Somta, Prakit
Molecular Analysis of Genetic Diversity and Structure of the Lablab (Lablab purpureus (L.) Sweet) Gene Pool Reveals Two Independent Routes of Domestication
title Molecular Analysis of Genetic Diversity and Structure of the Lablab (Lablab purpureus (L.) Sweet) Gene Pool Reveals Two Independent Routes of Domestication
title_full Molecular Analysis of Genetic Diversity and Structure of the Lablab (Lablab purpureus (L.) Sweet) Gene Pool Reveals Two Independent Routes of Domestication
title_fullStr Molecular Analysis of Genetic Diversity and Structure of the Lablab (Lablab purpureus (L.) Sweet) Gene Pool Reveals Two Independent Routes of Domestication
title_full_unstemmed Molecular Analysis of Genetic Diversity and Structure of the Lablab (Lablab purpureus (L.) Sweet) Gene Pool Reveals Two Independent Routes of Domestication
title_short Molecular Analysis of Genetic Diversity and Structure of the Lablab (Lablab purpureus (L.) Sweet) Gene Pool Reveals Two Independent Routes of Domestication
title_sort molecular analysis of genetic diversity and structure of the lablab (lablab purpureus (l.) sweet) gene pool reveals two independent routes of domestication
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824144/
https://www.ncbi.nlm.nih.gov/pubmed/36616186
http://dx.doi.org/10.3390/plants12010057
work_keys_str_mv AT kongjaimunalisa molecularanalysisofgeneticdiversityandstructureofthelablablablabpurpureuslsweetgenepoolrevealstwoindependentroutesofdomestication
AT takahashiyu molecularanalysisofgeneticdiversityandstructureofthelablablablabpurpureuslsweetgenepoolrevealstwoindependentroutesofdomestication
AT yoshiokayosuke molecularanalysisofgeneticdiversityandstructureofthelablablablabpurpureuslsweetgenepoolrevealstwoindependentroutesofdomestication
AT tomookanorihiko molecularanalysisofgeneticdiversityandstructureofthelablablablabpurpureuslsweetgenepoolrevealstwoindependentroutesofdomestication
AT mongkolrachsawan molecularanalysisofgeneticdiversityandstructureofthelablablablabpurpureuslsweetgenepoolrevealstwoindependentroutesofdomestication
AT somtaprakit molecularanalysisofgeneticdiversityandstructureofthelablablablabpurpureuslsweetgenepoolrevealstwoindependentroutesofdomestication