Cargando…
Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma
Biotin, spermine, and folic acid were covalently linked to the F127 copolymer to obtain a new drug delivery system designed for HY-loaded PDT treatment against B(16)F(10) cells. Chemical structures and binders quantification were performed by spectroscopy and spectrophotometric techniques ((1)NMR, H...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824163/ https://www.ncbi.nlm.nih.gov/pubmed/36616529 http://dx.doi.org/10.3390/polym15010179 |
_version_ | 1784866342025822208 |
---|---|
author | de Morais, Flávia Amanda Pedroso De Oliveira, Ana Carolina Vieira Balbinot, Rodolfo Bento Lazarin-Bidóia, Danielle Ueda-Nakamura, Tânia de Oliveira Silva, Sueli da Silva Souza Campanholi, Katieli da Silva Junior, Ranulfo Combuca Gonçalves, Renato Sonchini Caetano, Wilker Nakamura, Celso Vataru |
author_facet | de Morais, Flávia Amanda Pedroso De Oliveira, Ana Carolina Vieira Balbinot, Rodolfo Bento Lazarin-Bidóia, Danielle Ueda-Nakamura, Tânia de Oliveira Silva, Sueli da Silva Souza Campanholi, Katieli da Silva Junior, Ranulfo Combuca Gonçalves, Renato Sonchini Caetano, Wilker Nakamura, Celso Vataru |
author_sort | de Morais, Flávia Amanda Pedroso |
collection | PubMed |
description | Biotin, spermine, and folic acid were covalently linked to the F127 copolymer to obtain a new drug delivery system designed for HY-loaded PDT treatment against B(16)F(10) cells. Chemical structures and binders quantification were performed by spectroscopy and spectrophotometric techniques ((1)NMR, HABA/Avidin reagent, fluorescamine assay). Critical micelle concentration, critical micelle temperature, size, polydispersity, and zeta potential indicate the hydrophobicity of the binders can influence the physicochemical parameters. Spermine-modified micelles showed fewer changes in their physical and chemical parameters than the F127 micelles without modification. Furthermore, zeta potential measurements suggest an increase in the physical stability of these carrier systems. The phototherapeutic potential was demonstrated using hypericin-loaded formulation against B(16)F(10) cells, which shows that the combination of the binders on F127 copolymer micelles enhances the photosensitizer uptake and potentializes the photodynamic activity. |
format | Online Article Text |
id | pubmed-9824163 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98241632023-01-08 Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma de Morais, Flávia Amanda Pedroso De Oliveira, Ana Carolina Vieira Balbinot, Rodolfo Bento Lazarin-Bidóia, Danielle Ueda-Nakamura, Tânia de Oliveira Silva, Sueli da Silva Souza Campanholi, Katieli da Silva Junior, Ranulfo Combuca Gonçalves, Renato Sonchini Caetano, Wilker Nakamura, Celso Vataru Polymers (Basel) Article Biotin, spermine, and folic acid were covalently linked to the F127 copolymer to obtain a new drug delivery system designed for HY-loaded PDT treatment against B(16)F(10) cells. Chemical structures and binders quantification were performed by spectroscopy and spectrophotometric techniques ((1)NMR, HABA/Avidin reagent, fluorescamine assay). Critical micelle concentration, critical micelle temperature, size, polydispersity, and zeta potential indicate the hydrophobicity of the binders can influence the physicochemical parameters. Spermine-modified micelles showed fewer changes in their physical and chemical parameters than the F127 micelles without modification. Furthermore, zeta potential measurements suggest an increase in the physical stability of these carrier systems. The phototherapeutic potential was demonstrated using hypericin-loaded formulation against B(16)F(10) cells, which shows that the combination of the binders on F127 copolymer micelles enhances the photosensitizer uptake and potentializes the photodynamic activity. MDPI 2022-12-30 /pmc/articles/PMC9824163/ /pubmed/36616529 http://dx.doi.org/10.3390/polym15010179 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article de Morais, Flávia Amanda Pedroso De Oliveira, Ana Carolina Vieira Balbinot, Rodolfo Bento Lazarin-Bidóia, Danielle Ueda-Nakamura, Tânia de Oliveira Silva, Sueli da Silva Souza Campanholi, Katieli da Silva Junior, Ranulfo Combuca Gonçalves, Renato Sonchini Caetano, Wilker Nakamura, Celso Vataru Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma |
title | Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma |
title_full | Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma |
title_fullStr | Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma |
title_full_unstemmed | Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma |
title_short | Multifunctional Nanoparticles as High-Efficient Targeted Hypericin System for Theranostic Melanoma |
title_sort | multifunctional nanoparticles as high-efficient targeted hypericin system for theranostic melanoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824163/ https://www.ncbi.nlm.nih.gov/pubmed/36616529 http://dx.doi.org/10.3390/polym15010179 |
work_keys_str_mv | AT demoraisflaviaamandapedroso multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT deoliveiraanacarolinavieira multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT balbinotrodolfobento multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT lazarinbidoiadanielle multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT uedanakamuratania multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT deoliveirasilvasueli multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT dasilvasouzacampanholikatieli multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT dasilvajuniorranulfocombuca multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT goncalvesrenatosonchini multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT caetanowilker multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma AT nakamuracelsovataru multifunctionalnanoparticlesashighefficienttargetedhypericinsystemfortheranosticmelanoma |