Cargando…
Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries
More than half-century ago, local rice varieties were collected from Indochinese countries (Cambodia, Thailand, Laos, and Vietnam). Of these, 162 local varieties were examined for 7 grain-size traits: seed length/width/thickness, brown rice length/width/thickness, and 100-seed weight. Since these tr...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824235/ https://www.ncbi.nlm.nih.gov/pubmed/36616262 http://dx.doi.org/10.3390/plants12010133 |
_version_ | 1784866359939694592 |
---|---|
author | Lim, Sathya Onoda, Anna Orn, Chhourn Iwamoto, Hiromu Ishikawa, Ryo Saito, Hiroki Sato, Yutaka Ishii, Takashige |
author_facet | Lim, Sathya Onoda, Anna Orn, Chhourn Iwamoto, Hiromu Ishikawa, Ryo Saito, Hiroki Sato, Yutaka Ishii, Takashige |
author_sort | Lim, Sathya |
collection | PubMed |
description | More than half-century ago, local rice varieties were collected from Indochinese countries (Cambodia, Thailand, Laos, and Vietnam). Of these, 162 local varieties were examined for 7 grain-size traits: seed length/width/thickness, brown rice length/width/thickness, and 100-seed weight. Since these traits varied considerably, a survey of functional mutations was performed in the genes related to these traits. In total, 19 markers (12 InDel and 7 dCAPS markers) were used to investigate the mutations at 14 grain-size loci of GW2, GS2, qLGY3, GS3, GL3.1, TGW3, GS5, GW5, GS6, TGW6, GW6a, GLW7, GL7, and GW8. Significant allele effects were observed with six markers detecting base substitution mutations at GW2 and GS3 and insertion/deletion mutations at GS5, GW5, and GW6a, suggesting that these mutations might have affected the grain trait and caused variation among local varieties in the Indochinese countries. In addition to grain size, the hull color, grain color, and glutinosity were also examined using a survey of loss-of-function mutations at major responsible loci. Most phenotypes were reflected based on functional mutations at these loci. Since the local varieties have wide genetic variation, they are important genetic resources for future rice breeding. |
format | Online Article Text |
id | pubmed-9824235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-98242352023-01-08 Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries Lim, Sathya Onoda, Anna Orn, Chhourn Iwamoto, Hiromu Ishikawa, Ryo Saito, Hiroki Sato, Yutaka Ishii, Takashige Plants (Basel) Article More than half-century ago, local rice varieties were collected from Indochinese countries (Cambodia, Thailand, Laos, and Vietnam). Of these, 162 local varieties were examined for 7 grain-size traits: seed length/width/thickness, brown rice length/width/thickness, and 100-seed weight. Since these traits varied considerably, a survey of functional mutations was performed in the genes related to these traits. In total, 19 markers (12 InDel and 7 dCAPS markers) were used to investigate the mutations at 14 grain-size loci of GW2, GS2, qLGY3, GS3, GL3.1, TGW3, GS5, GW5, GS6, TGW6, GW6a, GLW7, GL7, and GW8. Significant allele effects were observed with six markers detecting base substitution mutations at GW2 and GS3 and insertion/deletion mutations at GS5, GW5, and GW6a, suggesting that these mutations might have affected the grain trait and caused variation among local varieties in the Indochinese countries. In addition to grain size, the hull color, grain color, and glutinosity were also examined using a survey of loss-of-function mutations at major responsible loci. Most phenotypes were reflected based on functional mutations at these loci. Since the local varieties have wide genetic variation, they are important genetic resources for future rice breeding. MDPI 2022-12-27 /pmc/articles/PMC9824235/ /pubmed/36616262 http://dx.doi.org/10.3390/plants12010133 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lim, Sathya Onoda, Anna Orn, Chhourn Iwamoto, Hiromu Ishikawa, Ryo Saito, Hiroki Sato, Yutaka Ishii, Takashige Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries |
title | Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries |
title_full | Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries |
title_fullStr | Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries |
title_full_unstemmed | Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries |
title_short | Variations in Grain Traits among Local Rice Varieties Collected More Than Half-Century Ago in Indochinese Countries |
title_sort | variations in grain traits among local rice varieties collected more than half-century ago in indochinese countries |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824235/ https://www.ncbi.nlm.nih.gov/pubmed/36616262 http://dx.doi.org/10.3390/plants12010133 |
work_keys_str_mv | AT limsathya variationsingraintraitsamonglocalricevarietiescollectedmorethanhalfcenturyagoinindochinesecountries AT onodaanna variationsingraintraitsamonglocalricevarietiescollectedmorethanhalfcenturyagoinindochinesecountries AT ornchhourn variationsingraintraitsamonglocalricevarietiescollectedmorethanhalfcenturyagoinindochinesecountries AT iwamotohiromu variationsingraintraitsamonglocalricevarietiescollectedmorethanhalfcenturyagoinindochinesecountries AT ishikawaryo variationsingraintraitsamonglocalricevarietiescollectedmorethanhalfcenturyagoinindochinesecountries AT saitohiroki variationsingraintraitsamonglocalricevarietiescollectedmorethanhalfcenturyagoinindochinesecountries AT satoyutaka variationsingraintraitsamonglocalricevarietiescollectedmorethanhalfcenturyagoinindochinesecountries AT ishiitakashige variationsingraintraitsamonglocalricevarietiescollectedmorethanhalfcenturyagoinindochinesecountries |