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Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity

Background: Immunoglobulin E (IgE)-mediated cow’s milk allergy (CMA) can be life-threatening and affects up to 3% of children. Hypoallergenic infant formulas based on hydrolyzed cow’s milk protein are increasingly considered for therapy and prevention of cow’s milk allergy. The aim of this study was...

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Autores principales: Freidl, Raphaela, Garib, Victoria, Linhart, Birgit, Haberl, Elisabeth M., Mader, Isabelle, Szépfalusi, Zsolt, Schmidthaler, Klara, Douladiris, Nikos, Pampura, Alexander, Varlamov, Evgeniy, Lepeshkova, Tatiana, Beltyukov, Evgeny, Naumova, Veronika, Taka, Styliani, Nosova, Dina, Guliashko, Olga, Kundi, Michael, Kiyamova, Alina, Katsamaki, Stefani, Valenta, Rudolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824366/
https://www.ncbi.nlm.nih.gov/pubmed/36615769
http://dx.doi.org/10.3390/nu15010111
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author Freidl, Raphaela
Garib, Victoria
Linhart, Birgit
Haberl, Elisabeth M.
Mader, Isabelle
Szépfalusi, Zsolt
Schmidthaler, Klara
Douladiris, Nikos
Pampura, Alexander
Varlamov, Evgeniy
Lepeshkova, Tatiana
Beltyukov, Evgeny
Naumova, Veronika
Taka, Styliani
Nosova, Dina
Guliashko, Olga
Kundi, Michael
Kiyamova, Alina
Katsamaki, Stefani
Valenta, Rudolf
author_facet Freidl, Raphaela
Garib, Victoria
Linhart, Birgit
Haberl, Elisabeth M.
Mader, Isabelle
Szépfalusi, Zsolt
Schmidthaler, Klara
Douladiris, Nikos
Pampura, Alexander
Varlamov, Evgeniy
Lepeshkova, Tatiana
Beltyukov, Evgeny
Naumova, Veronika
Taka, Styliani
Nosova, Dina
Guliashko, Olga
Kundi, Michael
Kiyamova, Alina
Katsamaki, Stefani
Valenta, Rudolf
author_sort Freidl, Raphaela
collection PubMed
description Background: Immunoglobulin E (IgE)-mediated cow’s milk allergy (CMA) can be life-threatening and affects up to 3% of children. Hypoallergenic infant formulas based on hydrolyzed cow’s milk protein are increasingly considered for therapy and prevention of cow’s milk allergy. The aim of this study was to investigate the allergenic activity and ability to induce T cell and cytokine responses of an infant formula based on extensively hydrolyzed cow’s milk protein (whey) (eHF, extensively hydrolyzed formula) supplemented with Galactooligosaccharides (GOS) and Limosilactobacillus fermentum CECT5716 (LF) to determine its suitability for treatment and prevention of CMA. Methods: eHF and standard protein formula based on intact cow’s milk proteins (iPF) with or without Galactooligosaccharide (GOS) and Limosilactobacillus fermentum CECT5716 (LF) were investigated with allergen-specific antibodies and tested for IgE reactivity and allergenic activity in basophil degranulation assays with sera from cow’s milk (CM)-allergic infants/children. Their ability to stimulate T cell proliferation and cytokine secretion in cultured peripheral blood mononuclear cells (PBMC) from CM-allergic infants and children was studied with a FACS-based carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution assay and xMAP Luminex fluorescent bead-based technology, respectively. Results: An eHF supplemented with GOS and LF exhibiting almost no IgE reactivity and allergenic activity was identified. This eHF induced significantly lower inflammatory cytokine secretion as compared to an intact protein-based infant formula but retained T cell reactivity. Conclusions: Due to strongly reduced allergenic activity and induction of inflammatory cytokine secretion but retained T cell reactivity, the identified eHF may be used for treatment and prevention of CMA by induction of specific T cell tolerance.
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spelling pubmed-98243662023-01-08 Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity Freidl, Raphaela Garib, Victoria Linhart, Birgit Haberl, Elisabeth M. Mader, Isabelle Szépfalusi, Zsolt Schmidthaler, Klara Douladiris, Nikos Pampura, Alexander Varlamov, Evgeniy Lepeshkova, Tatiana Beltyukov, Evgeny Naumova, Veronika Taka, Styliani Nosova, Dina Guliashko, Olga Kundi, Michael Kiyamova, Alina Katsamaki, Stefani Valenta, Rudolf Nutrients Article Background: Immunoglobulin E (IgE)-mediated cow’s milk allergy (CMA) can be life-threatening and affects up to 3% of children. Hypoallergenic infant formulas based on hydrolyzed cow’s milk protein are increasingly considered for therapy and prevention of cow’s milk allergy. The aim of this study was to investigate the allergenic activity and ability to induce T cell and cytokine responses of an infant formula based on extensively hydrolyzed cow’s milk protein (whey) (eHF, extensively hydrolyzed formula) supplemented with Galactooligosaccharides (GOS) and Limosilactobacillus fermentum CECT5716 (LF) to determine its suitability for treatment and prevention of CMA. Methods: eHF and standard protein formula based on intact cow’s milk proteins (iPF) with or without Galactooligosaccharide (GOS) and Limosilactobacillus fermentum CECT5716 (LF) were investigated with allergen-specific antibodies and tested for IgE reactivity and allergenic activity in basophil degranulation assays with sera from cow’s milk (CM)-allergic infants/children. Their ability to stimulate T cell proliferation and cytokine secretion in cultured peripheral blood mononuclear cells (PBMC) from CM-allergic infants and children was studied with a FACS-based carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution assay and xMAP Luminex fluorescent bead-based technology, respectively. Results: An eHF supplemented with GOS and LF exhibiting almost no IgE reactivity and allergenic activity was identified. This eHF induced significantly lower inflammatory cytokine secretion as compared to an intact protein-based infant formula but retained T cell reactivity. Conclusions: Due to strongly reduced allergenic activity and induction of inflammatory cytokine secretion but retained T cell reactivity, the identified eHF may be used for treatment and prevention of CMA by induction of specific T cell tolerance. MDPI 2022-12-26 /pmc/articles/PMC9824366/ /pubmed/36615769 http://dx.doi.org/10.3390/nu15010111 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Freidl, Raphaela
Garib, Victoria
Linhart, Birgit
Haberl, Elisabeth M.
Mader, Isabelle
Szépfalusi, Zsolt
Schmidthaler, Klara
Douladiris, Nikos
Pampura, Alexander
Varlamov, Evgeniy
Lepeshkova, Tatiana
Beltyukov, Evgeny
Naumova, Veronika
Taka, Styliani
Nosova, Dina
Guliashko, Olga
Kundi, Michael
Kiyamova, Alina
Katsamaki, Stefani
Valenta, Rudolf
Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity
title Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity
title_full Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity
title_fullStr Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity
title_full_unstemmed Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity
title_short Extensively Hydrolyzed Hypoallergenic Infant Formula with Retained T Cell Reactivity
title_sort extensively hydrolyzed hypoallergenic infant formula with retained t cell reactivity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824366/
https://www.ncbi.nlm.nih.gov/pubmed/36615769
http://dx.doi.org/10.3390/nu15010111
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