Cargando…

Genome-Wide Identification of PIN and PILS Gene Families in Areca catechu and the Potential Role of AcPIN6 in Lateral Brace Root Formation

PIN-FORMED (PIN) and PIN-LIKES (PILS) are two families of auxin transporters that control the directional cell-to-cell transport and intracellular accumulation of auxin, thereby influencing plant growth and development. Most knowledge of PINs and PILSs was obtained from the dicot model plant Arabido...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yicheng, Zhou, Guangzhen, Luo, Haifen, Li, Xinyu, Zhang, Kelan, Wan, Yinglang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824567/
https://www.ncbi.nlm.nih.gov/pubmed/36616161
http://dx.doi.org/10.3390/plants12010033
_version_ 1784866441891151872
author Wang, Yicheng
Zhou, Guangzhen
Luo, Haifen
Li, Xinyu
Zhang, Kelan
Wan, Yinglang
author_facet Wang, Yicheng
Zhou, Guangzhen
Luo, Haifen
Li, Xinyu
Zhang, Kelan
Wan, Yinglang
author_sort Wang, Yicheng
collection PubMed
description PIN-FORMED (PIN) and PIN-LIKES (PILS) are two families of auxin transporters that control the directional cell-to-cell transport and intracellular accumulation of auxin, thereby influencing plant growth and development. Most knowledge of PINs and PILSs was obtained from the dicot model plant Arabidopsis thaliana. Here, we focus on the distribution and expression of the PIN and PILS gene families in areca palm (Areca catechu), a monocot tree. The whole genomic dataset of areca palm was used to identify twelve AcPINs and eight AcPILSs, and a phylogenetic tree was constructed of PINS and PILS together with several other palm species, including the date palm (Phoenix dactylifera), oil palm (Elaeis guineensis), and coconut (Cocos nucifera). We further analyzed the expression patterns of AcPIN and AcPILS in areca palm, and found that AcPIN6 displayed an extremely high transcriptional abundance in the brace roots and was extremely stimulated in the lateral root primordium. This result implies that AcPIN6 plays an important role in the growth and formation of brace roots, especially in lateral root initiation. We also overexpressed AcPIN6 and AcPIN6–eGFP in Arabidopsis, and the results revealed that the PIN6 localized on the plasma membrane and affected auxin-related phenomena. Taken together, we analyzed the evolutionary relationships of PINs and PILSs in palm species, and the roles of PIN6 in areca palm root formation. The results will improve the understanding of root system construction in large palm trees.
format Online
Article
Text
id pubmed-9824567
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-98245672023-01-08 Genome-Wide Identification of PIN and PILS Gene Families in Areca catechu and the Potential Role of AcPIN6 in Lateral Brace Root Formation Wang, Yicheng Zhou, Guangzhen Luo, Haifen Li, Xinyu Zhang, Kelan Wan, Yinglang Plants (Basel) Article PIN-FORMED (PIN) and PIN-LIKES (PILS) are two families of auxin transporters that control the directional cell-to-cell transport and intracellular accumulation of auxin, thereby influencing plant growth and development. Most knowledge of PINs and PILSs was obtained from the dicot model plant Arabidopsis thaliana. Here, we focus on the distribution and expression of the PIN and PILS gene families in areca palm (Areca catechu), a monocot tree. The whole genomic dataset of areca palm was used to identify twelve AcPINs and eight AcPILSs, and a phylogenetic tree was constructed of PINS and PILS together with several other palm species, including the date palm (Phoenix dactylifera), oil palm (Elaeis guineensis), and coconut (Cocos nucifera). We further analyzed the expression patterns of AcPIN and AcPILS in areca palm, and found that AcPIN6 displayed an extremely high transcriptional abundance in the brace roots and was extremely stimulated in the lateral root primordium. This result implies that AcPIN6 plays an important role in the growth and formation of brace roots, especially in lateral root initiation. We also overexpressed AcPIN6 and AcPIN6–eGFP in Arabidopsis, and the results revealed that the PIN6 localized on the plasma membrane and affected auxin-related phenomena. Taken together, we analyzed the evolutionary relationships of PINs and PILSs in palm species, and the roles of PIN6 in areca palm root formation. The results will improve the understanding of root system construction in large palm trees. MDPI 2022-12-21 /pmc/articles/PMC9824567/ /pubmed/36616161 http://dx.doi.org/10.3390/plants12010033 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yicheng
Zhou, Guangzhen
Luo, Haifen
Li, Xinyu
Zhang, Kelan
Wan, Yinglang
Genome-Wide Identification of PIN and PILS Gene Families in Areca catechu and the Potential Role of AcPIN6 in Lateral Brace Root Formation
title Genome-Wide Identification of PIN and PILS Gene Families in Areca catechu and the Potential Role of AcPIN6 in Lateral Brace Root Formation
title_full Genome-Wide Identification of PIN and PILS Gene Families in Areca catechu and the Potential Role of AcPIN6 in Lateral Brace Root Formation
title_fullStr Genome-Wide Identification of PIN and PILS Gene Families in Areca catechu and the Potential Role of AcPIN6 in Lateral Brace Root Formation
title_full_unstemmed Genome-Wide Identification of PIN and PILS Gene Families in Areca catechu and the Potential Role of AcPIN6 in Lateral Brace Root Formation
title_short Genome-Wide Identification of PIN and PILS Gene Families in Areca catechu and the Potential Role of AcPIN6 in Lateral Brace Root Formation
title_sort genome-wide identification of pin and pils gene families in areca catechu and the potential role of acpin6 in lateral brace root formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824567/
https://www.ncbi.nlm.nih.gov/pubmed/36616161
http://dx.doi.org/10.3390/plants12010033
work_keys_str_mv AT wangyicheng genomewideidentificationofpinandpilsgenefamiliesinarecacatechuandthepotentialroleofacpin6inlateralbracerootformation
AT zhouguangzhen genomewideidentificationofpinandpilsgenefamiliesinarecacatechuandthepotentialroleofacpin6inlateralbracerootformation
AT luohaifen genomewideidentificationofpinandpilsgenefamiliesinarecacatechuandthepotentialroleofacpin6inlateralbracerootformation
AT lixinyu genomewideidentificationofpinandpilsgenefamiliesinarecacatechuandthepotentialroleofacpin6inlateralbracerootformation
AT zhangkelan genomewideidentificationofpinandpilsgenefamiliesinarecacatechuandthepotentialroleofacpin6inlateralbracerootformation
AT wanyinglang genomewideidentificationofpinandpilsgenefamiliesinarecacatechuandthepotentialroleofacpin6inlateralbracerootformation