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Assessing the Contribution of the Neutral Blocks in DNA/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide)

The interaction of DNA with different block copolymers, namely poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(acrylamide), i.e., (PTEA)-b-(PAm), and poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(ethylene oxide), i.e., (PTEA)-b-(PEO), was studied. The nature of...

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Autores principales: Giona, Renata Mello, Vitorazi, Letícia, Loh, Watson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824764/
https://www.ncbi.nlm.nih.gov/pubmed/36615592
http://dx.doi.org/10.3390/molecules28010398
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author Giona, Renata Mello
Vitorazi, Letícia
Loh, Watson
author_facet Giona, Renata Mello
Vitorazi, Letícia
Loh, Watson
author_sort Giona, Renata Mello
collection PubMed
description The interaction of DNA with different block copolymers, namely poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(acrylamide), i.e., (PTEA)-b-(PAm), and poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(ethylene oxide), i.e., (PTEA)-b-(PEO), was studied. The nature of the cationic block was maintained fixed (PTEA), whereas the neutral blocks contained varying amounts of acrylamide or (ethylene oxide) units. According to results from isothermal titration microcalorimetry measurements, the copolymers interaction with DNA is endothermic with an enthalpy around 4.0 kJ mol(−1) of charges for (PTEA)-b-(PAm) and 5.5 kJ mol(−1) of charges for (PTEA)-b-(PEO). The hydrodynamic diameters of (PTEA)-b-(PEO)/DNA and (PTEA)-b-(PAm)/DNA polyplexes prepared by titration were around 200 nm at charge ratio (Z(+/−)) < 1. At Z(+/−) close and above 1, the (PTEA)(50)-b-(PAm)(50)/DNA and (PTEA)(50)-b-(PAm)(200)/DNA polyplexes precipitated. Interestingly, (PTEA)(50)-b-(PAm)(1000)/DNA polyplexes remained with a size of around 300 nm even after charge neutralization, probably due to the size of the neutral block. Conversely, for (PTEA)(96)-b-(PEO)(100)/DNA polyplexes, the size distribution was broad, indicating a more heterogeneous system. Polyplexes were also prepared by direct mixture at Z(+/−) of 2.0, and they displayed diameters around 120–150 nm, remaining stable for more than 10 days. Direct and reverse titration experiments showed that the order of addition affects both the size and charge of the resulting polyplexes.
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spelling pubmed-98247642023-01-08 Assessing the Contribution of the Neutral Blocks in DNA/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide) Giona, Renata Mello Vitorazi, Letícia Loh, Watson Molecules Article The interaction of DNA with different block copolymers, namely poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(acrylamide), i.e., (PTEA)-b-(PAm), and poly (trimethylammonium chloride methacryloyoxy)ethyl)-block-poly(ethylene oxide), i.e., (PTEA)-b-(PEO), was studied. The nature of the cationic block was maintained fixed (PTEA), whereas the neutral blocks contained varying amounts of acrylamide or (ethylene oxide) units. According to results from isothermal titration microcalorimetry measurements, the copolymers interaction with DNA is endothermic with an enthalpy around 4.0 kJ mol(−1) of charges for (PTEA)-b-(PAm) and 5.5 kJ mol(−1) of charges for (PTEA)-b-(PEO). The hydrodynamic diameters of (PTEA)-b-(PEO)/DNA and (PTEA)-b-(PAm)/DNA polyplexes prepared by titration were around 200 nm at charge ratio (Z(+/−)) < 1. At Z(+/−) close and above 1, the (PTEA)(50)-b-(PAm)(50)/DNA and (PTEA)(50)-b-(PAm)(200)/DNA polyplexes precipitated. Interestingly, (PTEA)(50)-b-(PAm)(1000)/DNA polyplexes remained with a size of around 300 nm even after charge neutralization, probably due to the size of the neutral block. Conversely, for (PTEA)(96)-b-(PEO)(100)/DNA polyplexes, the size distribution was broad, indicating a more heterogeneous system. Polyplexes were also prepared by direct mixture at Z(+/−) of 2.0, and they displayed diameters around 120–150 nm, remaining stable for more than 10 days. Direct and reverse titration experiments showed that the order of addition affects both the size and charge of the resulting polyplexes. MDPI 2023-01-03 /pmc/articles/PMC9824764/ /pubmed/36615592 http://dx.doi.org/10.3390/molecules28010398 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giona, Renata Mello
Vitorazi, Letícia
Loh, Watson
Assessing the Contribution of the Neutral Blocks in DNA/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide)
title Assessing the Contribution of the Neutral Blocks in DNA/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide)
title_full Assessing the Contribution of the Neutral Blocks in DNA/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide)
title_fullStr Assessing the Contribution of the Neutral Blocks in DNA/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide)
title_full_unstemmed Assessing the Contribution of the Neutral Blocks in DNA/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide)
title_short Assessing the Contribution of the Neutral Blocks in DNA/Block-Copolymer Polyplexes: Poly(acrylamide) vs. Poly(ethylene Oxide)
title_sort assessing the contribution of the neutral blocks in dna/block-copolymer polyplexes: poly(acrylamide) vs. poly(ethylene oxide)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9824764/
https://www.ncbi.nlm.nih.gov/pubmed/36615592
http://dx.doi.org/10.3390/molecules28010398
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