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Improved genome editing by an engineered CRISPR-Cas12a

CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single catalytic site to both cleave target double-stranded DNA (dsDNA) (cis-activity) and indiscriminately degrade single-stranded DNA (ssDNA) (...

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Detalles Bibliográficos
Autores principales:	Ma, Enbo, Chen, Kai, Shi, Honglue, Stahl, Elizabeth C, Adler, Ben, Trinidad, Marena, Liu, Junjie, Zhou, Kaihong, Ye, Jinjuan, Doudna, Jennifer A
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Chemical Biology and Nucleic Acid Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825149/ https://www.ncbi.nlm.nih.gov/pubmed/36537251 http://dx.doi.org/10.1093/nar/gkac1192

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