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The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation

The model plant Arabidopsis thaliana encodes as many as ten Argonaute proteins (AGO1–10) with different functions. Each AGO selectively loads a set of small RNAs by recognizing their length and 5′ nucleotide identity to properly regulate target genes. Previous studies showed that AGO4 and AGO6, key...

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Autores principales: Liu, Wei, Shoji, Keisuke, Naganuma, Masahiro, Tomari, Yukihide, Iwakawa, Hiro-oki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825178/
https://www.ncbi.nlm.nih.gov/pubmed/36477368
http://dx.doi.org/10.1093/nar/gkac1135
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author Liu, Wei
Shoji, Keisuke
Naganuma, Masahiro
Tomari, Yukihide
Iwakawa, Hiro-oki
author_facet Liu, Wei
Shoji, Keisuke
Naganuma, Masahiro
Tomari, Yukihide
Iwakawa, Hiro-oki
author_sort Liu, Wei
collection PubMed
description The model plant Arabidopsis thaliana encodes as many as ten Argonaute proteins (AGO1–10) with different functions. Each AGO selectively loads a set of small RNAs by recognizing their length and 5′ nucleotide identity to properly regulate target genes. Previous studies showed that AGO4 and AGO6, key factors in DNA methylation, incorporate 24-nt small-interfering RNAs with 5′ adenine (24A siRNAs). However, it has been unclear how these AGOs specifically load 24A siRNAs. Here, we biochemically investigated the siRNA preference of AGO4, AGO6 and their chimeric mutants. We found that AGO4 and AGO6 use distinct mechanisms to preferentially load 24A siRNAs. Moreover, we showed that the 5′ A specificity of AGO4 and AGO6 is not determined by the previously known nucleotide specificity loop in the MID domain but rather by the coordination of the MID and PIWI domains. These findings advance our mechanistic understanding of how small RNAs are accurately sorted into different AGO proteins in plants.
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spelling pubmed-98251782023-01-09 The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation Liu, Wei Shoji, Keisuke Naganuma, Masahiro Tomari, Yukihide Iwakawa, Hiro-oki Nucleic Acids Res RNA and RNA-protein complexes The model plant Arabidopsis thaliana encodes as many as ten Argonaute proteins (AGO1–10) with different functions. Each AGO selectively loads a set of small RNAs by recognizing their length and 5′ nucleotide identity to properly regulate target genes. Previous studies showed that AGO4 and AGO6, key factors in DNA methylation, incorporate 24-nt small-interfering RNAs with 5′ adenine (24A siRNAs). However, it has been unclear how these AGOs specifically load 24A siRNAs. Here, we biochemically investigated the siRNA preference of AGO4, AGO6 and their chimeric mutants. We found that AGO4 and AGO6 use distinct mechanisms to preferentially load 24A siRNAs. Moreover, we showed that the 5′ A specificity of AGO4 and AGO6 is not determined by the previously known nucleotide specificity loop in the MID domain but rather by the coordination of the MID and PIWI domains. These findings advance our mechanistic understanding of how small RNAs are accurately sorted into different AGO proteins in plants. Oxford University Press 2022-12-07 /pmc/articles/PMC9825178/ /pubmed/36477368 http://dx.doi.org/10.1093/nar/gkac1135 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA and RNA-protein complexes
Liu, Wei
Shoji, Keisuke
Naganuma, Masahiro
Tomari, Yukihide
Iwakawa, Hiro-oki
The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation
title The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation
title_full The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation
title_fullStr The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation
title_full_unstemmed The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation
title_short The mechanisms of siRNA selection by plant Argonaute proteins triggering DNA methylation
title_sort mechanisms of sirna selection by plant argonaute proteins triggering dna methylation
topic RNA and RNA-protein complexes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825178/
https://www.ncbi.nlm.nih.gov/pubmed/36477368
http://dx.doi.org/10.1093/nar/gkac1135
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