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Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing
The amount of single-cell RNA-sequencing (scRNA-seq) data produced in the field of human male reproduction has steadily increased. Transcriptional profiles of thousands of testicular cells have been generated covering the human neonatal, prepubertal, pubertal and adult period as well as different ty...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825264/ https://www.ncbi.nlm.nih.gov/pubmed/36409992 http://dx.doi.org/10.1093/humrep/deac245 |
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author | Di Persio, Sara Neuhaus, Nina |
author_facet | Di Persio, Sara Neuhaus, Nina |
author_sort | Di Persio, Sara |
collection | PubMed |
description | The amount of single-cell RNA-sequencing (scRNA-seq) data produced in the field of human male reproduction has steadily increased. Transcriptional profiles of thousands of testicular cells have been generated covering the human neonatal, prepubertal, pubertal and adult period as well as different types of male infertility; the latter include non-obstructive azoospermia, cryptozoospermia, Klinefelter syndrome and azoospermia factor deletions. In this review, we provide an overview of transcriptional changes in different testicular subpopulations during postnatal development and in cases of male infertility. Moreover, we review novel concepts regarding the existence of spermatogonial and somatic cell subtypes as well as their crosstalk and provide corresponding marker genes to facilitate their identification. We discuss the potential clinical implications of scRNA-seq findings, the need for spatial information and the necessity to corroborate findings by exploring other levels of regulation, including at the epigenetic or protein level. |
format | Online Article Text |
id | pubmed-9825264 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-98252642023-01-09 Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing Di Persio, Sara Neuhaus, Nina Hum Reprod Mini-Review The amount of single-cell RNA-sequencing (scRNA-seq) data produced in the field of human male reproduction has steadily increased. Transcriptional profiles of thousands of testicular cells have been generated covering the human neonatal, prepubertal, pubertal and adult period as well as different types of male infertility; the latter include non-obstructive azoospermia, cryptozoospermia, Klinefelter syndrome and azoospermia factor deletions. In this review, we provide an overview of transcriptional changes in different testicular subpopulations during postnatal development and in cases of male infertility. Moreover, we review novel concepts regarding the existence of spermatogonial and somatic cell subtypes as well as their crosstalk and provide corresponding marker genes to facilitate their identification. We discuss the potential clinical implications of scRNA-seq findings, the need for spatial information and the necessity to corroborate findings by exploring other levels of regulation, including at the epigenetic or protein level. Oxford University Press 2022-11-21 /pmc/articles/PMC9825264/ /pubmed/36409992 http://dx.doi.org/10.1093/humrep/deac245 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Mini-Review Di Persio, Sara Neuhaus, Nina Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing |
title | Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing |
title_full | Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing |
title_fullStr | Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing |
title_full_unstemmed | Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing |
title_short | Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing |
title_sort | human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell rna-sequencing |
topic | Mini-Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825264/ https://www.ncbi.nlm.nih.gov/pubmed/36409992 http://dx.doi.org/10.1093/humrep/deac245 |
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