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Searching for a Consensus Among Inflammatory Bowel Disease Studies: A Systematic Meta-Analysis
BACKGROUND: Numerous studies have examined the gut microbial ecology of patients with Crohn’s disease (CD) and ulcerative colitis, but inflammatory bowel disease–associated taxa and ecological effect sizes are not consistent between studies. METHODS: We systematically searched PubMed and Google Scho...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825291/ https://www.ncbi.nlm.nih.gov/pubmed/36112501 http://dx.doi.org/10.1093/ibd/izac194 |
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author | Abdel-Rahman, Lama Izzat Hasan Morgan, Xochitl C |
author_facet | Abdel-Rahman, Lama Izzat Hasan Morgan, Xochitl C |
author_sort | Abdel-Rahman, Lama Izzat Hasan |
collection | PubMed |
description | BACKGROUND: Numerous studies have examined the gut microbial ecology of patients with Crohn’s disease (CD) and ulcerative colitis, but inflammatory bowel disease–associated taxa and ecological effect sizes are not consistent between studies. METHODS: We systematically searched PubMed and Google Scholar and performed a meta-analysis of 13 studies to analyze how variables such as sample type (stool, biopsy, and lavage) affect results in inflammatory bowel disease gut microbiome studies, using uniform bioinformatic methods for all primary data. RESULTS: Reduced alpha diversity was a consistent feature of both CD and ulcerative colitis but was more pronounced in CD. Disease contributed significantly variation in beta diversity in most studies, but effect size varied, and the effect of sample type was greater than the effect of disease. Fusobacterium was the genus most consistently associated with CD, but disease-associated genera were mostly inconsistent between studies. Stool studies had lower heterogeneity than biopsy studies, especially for CD. CONCLUSIONS: Our results indicate that sample type variation is an important contributor to study variability that should be carefully considered during study design, and stool is likely superior to biopsy for CD studies due to its lower heterogeneity. |
format | Online Article Text |
id | pubmed-9825291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-98252912023-01-09 Searching for a Consensus Among Inflammatory Bowel Disease Studies: A Systematic Meta-Analysis Abdel-Rahman, Lama Izzat Hasan Morgan, Xochitl C Inflamm Bowel Dis Basic Science Research BACKGROUND: Numerous studies have examined the gut microbial ecology of patients with Crohn’s disease (CD) and ulcerative colitis, but inflammatory bowel disease–associated taxa and ecological effect sizes are not consistent between studies. METHODS: We systematically searched PubMed and Google Scholar and performed a meta-analysis of 13 studies to analyze how variables such as sample type (stool, biopsy, and lavage) affect results in inflammatory bowel disease gut microbiome studies, using uniform bioinformatic methods for all primary data. RESULTS: Reduced alpha diversity was a consistent feature of both CD and ulcerative colitis but was more pronounced in CD. Disease contributed significantly variation in beta diversity in most studies, but effect size varied, and the effect of sample type was greater than the effect of disease. Fusobacterium was the genus most consistently associated with CD, but disease-associated genera were mostly inconsistent between studies. Stool studies had lower heterogeneity than biopsy studies, especially for CD. CONCLUSIONS: Our results indicate that sample type variation is an important contributor to study variability that should be carefully considered during study design, and stool is likely superior to biopsy for CD studies due to its lower heterogeneity. Oxford University Press 2022-09-16 /pmc/articles/PMC9825291/ /pubmed/36112501 http://dx.doi.org/10.1093/ibd/izac194 Text en © 2022 Crohn’s & Colitis Foundation. Published by Oxford University Press on behalf of Crohn’s & Colitis Foundation. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Basic Science Research Abdel-Rahman, Lama Izzat Hasan Morgan, Xochitl C Searching for a Consensus Among Inflammatory Bowel Disease Studies: A Systematic Meta-Analysis |
title | Searching for a Consensus Among Inflammatory Bowel Disease Studies: A Systematic Meta-Analysis |
title_full | Searching for a Consensus Among Inflammatory Bowel Disease Studies: A Systematic Meta-Analysis |
title_fullStr | Searching for a Consensus Among Inflammatory Bowel Disease Studies: A Systematic Meta-Analysis |
title_full_unstemmed | Searching for a Consensus Among Inflammatory Bowel Disease Studies: A Systematic Meta-Analysis |
title_short | Searching for a Consensus Among Inflammatory Bowel Disease Studies: A Systematic Meta-Analysis |
title_sort | searching for a consensus among inflammatory bowel disease studies: a systematic meta-analysis |
topic | Basic Science Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825291/ https://www.ncbi.nlm.nih.gov/pubmed/36112501 http://dx.doi.org/10.1093/ibd/izac194 |
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