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Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins

The plant hormone gibberellin (GA) impacts plant growth and development differently depending on the developmental context. In the maize (Zea mays) tassel, application of GA alters floral development, resulting in the persistence of pistils. GA signaling is achieved by the GA‐dependent turnover of D...

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Autores principales: Best, Norman B., Dilkes, Brian P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826531/
https://www.ncbi.nlm.nih.gov/pubmed/36050832
http://dx.doi.org/10.1111/tpj.15961
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author Best, Norman B.
Dilkes, Brian P.
author_facet Best, Norman B.
Dilkes, Brian P.
author_sort Best, Norman B.
collection PubMed
description The plant hormone gibberellin (GA) impacts plant growth and development differently depending on the developmental context. In the maize (Zea mays) tassel, application of GA alters floral development, resulting in the persistence of pistils. GA signaling is achieved by the GA‐dependent turnover of DELLA domain transcription factors, encoded by dwarf8 (d8) and dwarf9 (d9) in maize. The D8‐Mpl and D9‐1 alleles disrupt GA signaling, resulting in short plants and normal tassel floret development in the presence of excess GA. However, D9‐1 mutants are unable to block GA‐induced pistil development. Gene expression in developing tassels of D8‐Mpl and D9‐1 mutants and their wild‐type siblings was determined upon excess GA(3) and mock treatments. Using GA‐sensitive transcripts as reporters of GA signaling, we identified a weak loss of repression under mock conditions in both mutants, with the effect in D9‐1 being greater. D9‐1 was also less able to repress GA signaling in the presence of excess GA(3). We treated a diverse set of maize inbred lines with excess GA(3) and measured the phenotypic consequences on multiple aspects of development (e.g., height and pistil persistence in tassel florets). Genotype affected all GA‐regulated phenotypes but there was no correlation between any of the GA‐affected phenotypes, indicating that the complexity of the relationship between GA and development extends beyond the two‐gene epistasis previously demonstrated for GA and brassinosteroid biosynthetic mutants.
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spelling pubmed-98265312023-01-09 Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins Best, Norman B. Dilkes, Brian P. Plant J Original Articles The plant hormone gibberellin (GA) impacts plant growth and development differently depending on the developmental context. In the maize (Zea mays) tassel, application of GA alters floral development, resulting in the persistence of pistils. GA signaling is achieved by the GA‐dependent turnover of DELLA domain transcription factors, encoded by dwarf8 (d8) and dwarf9 (d9) in maize. The D8‐Mpl and D9‐1 alleles disrupt GA signaling, resulting in short plants and normal tassel floret development in the presence of excess GA. However, D9‐1 mutants are unable to block GA‐induced pistil development. Gene expression in developing tassels of D8‐Mpl and D9‐1 mutants and their wild‐type siblings was determined upon excess GA(3) and mock treatments. Using GA‐sensitive transcripts as reporters of GA signaling, we identified a weak loss of repression under mock conditions in both mutants, with the effect in D9‐1 being greater. D9‐1 was also less able to repress GA signaling in the presence of excess GA(3). We treated a diverse set of maize inbred lines with excess GA(3) and measured the phenotypic consequences on multiple aspects of development (e.g., height and pistil persistence in tassel florets). Genotype affected all GA‐regulated phenotypes but there was no correlation between any of the GA‐affected phenotypes, indicating that the complexity of the relationship between GA and development extends beyond the two‐gene epistasis previously demonstrated for GA and brassinosteroid biosynthetic mutants. John Wiley and Sons Inc. 2022-09-14 2022-10 /pmc/articles/PMC9826531/ /pubmed/36050832 http://dx.doi.org/10.1111/tpj.15961 Text en © 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Best, Norman B.
Dilkes, Brian P.
Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins
title Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins
title_full Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins
title_fullStr Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins
title_full_unstemmed Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins
title_short Transcriptional responses to gibberellin in the maize tassel and control by DELLA domain proteins
title_sort transcriptional responses to gibberellin in the maize tassel and control by della domain proteins
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826531/
https://www.ncbi.nlm.nih.gov/pubmed/36050832
http://dx.doi.org/10.1111/tpj.15961
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