Differentiating PC12 cells to evaluate neurite densities through live-cell imaging

Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate PC...

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Detalles Bibliográficos
Autores principales: Karliner, Jordyn, Merry, Diane E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826846/
https://www.ncbi.nlm.nih.gov/pubmed/36602900
http://dx.doi.org/10.1016/j.xpro.2022.101993
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author Karliner, Jordyn
Merry, Diane E.
author_facet Karliner, Jordyn
Merry, Diane E.
author_sort Karliner, Jordyn
collection PubMed
description Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate PC12 cells into equivalent neurite densities through live-cell imaging for morphological, immunocytochemical, and biochemical analyses. We detail steps on optimized substrate coating, plating techniques, culture media, validation steps, and quantification techniques.
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spelling pubmed-98268462023-01-10 Differentiating PC12 cells to evaluate neurite densities through live-cell imaging Karliner, Jordyn Merry, Diane E. STAR Protoc Protocol Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate PC12 cells into equivalent neurite densities through live-cell imaging for morphological, immunocytochemical, and biochemical analyses. We detail steps on optimized substrate coating, plating techniques, culture media, validation steps, and quantification techniques. Elsevier 2023-01-04 /pmc/articles/PMC9826846/ /pubmed/36602900 http://dx.doi.org/10.1016/j.xpro.2022.101993 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Karliner, Jordyn
Merry, Diane E.
Differentiating PC12 cells to evaluate neurite densities through live-cell imaging
title Differentiating PC12 cells to evaluate neurite densities through live-cell imaging
title_full Differentiating PC12 cells to evaluate neurite densities through live-cell imaging
title_fullStr Differentiating PC12 cells to evaluate neurite densities through live-cell imaging
title_full_unstemmed Differentiating PC12 cells to evaluate neurite densities through live-cell imaging
title_short Differentiating PC12 cells to evaluate neurite densities through live-cell imaging
title_sort differentiating pc12 cells to evaluate neurite densities through live-cell imaging
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826846/
https://www.ncbi.nlm.nih.gov/pubmed/36602900
http://dx.doi.org/10.1016/j.xpro.2022.101993
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