Protocol to detect antigen-specific nasal-resident T cells in humans

Specialized T cells are located in the nasal cavity and act as the first line of defense against respiratory viral infection. Here, we present a protocol for the detection and characterization of antigen-specific nasal-resident T cells. We detail steps for localized nasal swabbing to collect the nas...

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Detalles Bibliográficos
Autores principales: Lim, Joey Ming Er, Tan, Anthony T., Bertoletti, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826858/
https://www.ncbi.nlm.nih.gov/pubmed/36602901
http://dx.doi.org/10.1016/j.xpro.2022.101995
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author Lim, Joey Ming Er
Tan, Anthony T.
Bertoletti, Antonio
author_facet Lim, Joey Ming Er
Tan, Anthony T.
Bertoletti, Antonio
author_sort Lim, Joey Ming Er
collection PubMed
description Specialized T cells are located in the nasal cavity and act as the first line of defense against respiratory viral infection. Here, we present a protocol for the detection and characterization of antigen-specific nasal-resident T cells. We detail steps for localized nasal swabbing to collect the nasal samples. We then describe IFN-γ ELISpot and an activation-induced marker assay to detect and characterize antigen-specific nasal-resident T cells. For complete details on the use and execution of this protocol, please refer to Lim et al. (2022).(1)
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spelling pubmed-98268582023-01-10 Protocol to detect antigen-specific nasal-resident T cells in humans Lim, Joey Ming Er Tan, Anthony T. Bertoletti, Antonio STAR Protoc Protocol Specialized T cells are located in the nasal cavity and act as the first line of defense against respiratory viral infection. Here, we present a protocol for the detection and characterization of antigen-specific nasal-resident T cells. We detail steps for localized nasal swabbing to collect the nasal samples. We then describe IFN-γ ELISpot and an activation-induced marker assay to detect and characterize antigen-specific nasal-resident T cells. For complete details on the use and execution of this protocol, please refer to Lim et al. (2022).(1) Elsevier 2023-01-04 /pmc/articles/PMC9826858/ /pubmed/36602901 http://dx.doi.org/10.1016/j.xpro.2022.101995 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lim, Joey Ming Er
Tan, Anthony T.
Bertoletti, Antonio
Protocol to detect antigen-specific nasal-resident T cells in humans
title Protocol to detect antigen-specific nasal-resident T cells in humans
title_full Protocol to detect antigen-specific nasal-resident T cells in humans
title_fullStr Protocol to detect antigen-specific nasal-resident T cells in humans
title_full_unstemmed Protocol to detect antigen-specific nasal-resident T cells in humans
title_short Protocol to detect antigen-specific nasal-resident T cells in humans
title_sort protocol to detect antigen-specific nasal-resident t cells in humans
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826858/
https://www.ncbi.nlm.nih.gov/pubmed/36602901
http://dx.doi.org/10.1016/j.xpro.2022.101995
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